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Ligand Activity Charts

vorinostat [Ligand Id: 6852] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL98 (MK-0683, MK0683, NSC-701852, NSC-748799, NSC-759852, Suberoylanilide hydroxamic acid, Vorinostat, Zolinza)
Amyloid-beta A4 protein in Human [ChEMBL: CHEMBL2487] [UniProtKB: P05067] There should be some charts here, you may need to enable JavaScript!
Apoptosis regulator BAX in Human [ChEMBL: CHEMBL5318] [UniProtKB: Q07812] There should be some charts here, you may need to enable JavaScript!
bromodomain containing 4/Bromodomain-containing protein 4 in Human [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885] There should be some charts here, you may need to enable JavaScript!
DNA methyltransferase 1/DNA (cytosine-5)-methyltransferase 1 in Human [ChEMBL: CHEMBL1993] [GtoPdb: 2605] [UniProtKB: P26358] There should be some charts here, you may need to enable JavaScript!
epidermal growth factor receptor/Epidermal growth factor receptor erbB1 in Human [ChEMBL: CHEMBL203] [GtoPdb: 1797] [UniProtKB: P00533] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 1/HDAC1/REST corepressor 3 in Human [ChEMBL: CHEMBL4296112] [GtoPdb: 2658] [UniProtKB: Q13547, Q9P2K3] There should be some charts here, you may need to enable JavaScript!
heat shock protein 90 alpha family class A member 1/Heat shock protein HSP 90-alpha in Human [ChEMBL: CHEMBL3880] [GtoPdb: 2905] [UniProtKB: P07900] There should be some charts here, you may need to enable JavaScript!
K_v11.1/HERG in Human [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase in Plasmodium falciparum [ChEMBL: CHEMBL5541] [UniProtKB: Q9XYC7] Plasmodium falciparum histone deacetylase 1/Histone deacetylase in Plasmodium falciparum 3D7 [ChEMBL: CHEMBL1293190] [GtoPdb: 3072] [UniProtKB: Q7K6A1] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 1/Histone deacetylase 1 in Human [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547] histone deacetylase 1/Histone deacetylase 1 in Mouse [ChEMBL: CHEMBL4001] [GtoPdb: 2658] [UniProtKB: O09106] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 10/Histone deacetylase 10 in Human [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 11/Histone deacetylase 11 in Human [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 2/Histone deacetylase 2 in Human [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3 in Human [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3/NCoR1 in Human [ChEMBL: CHEMBL3038484] [GtoPdb: 2617] [UniProtKB: O15379, O75376] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3/Nuclear receptor corepressor 2 (HDAC3/NCoR2) in Human [ChEMBL: CHEMBL2111363] [GtoPdb: 2617] [UniProtKB: O15379, Q9Y618] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 4/Histone deacetylase 4 in Human [ChEMBL: CHEMBL3524] [GtoPdb: 2659] [UniProtKB: P56524] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 5/Histone deacetylase 5 in Human [ChEMBL: CHEMBL2563] [GtoPdb: 2660] [UniProtKB: Q9UQL6] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 7/Histone deacetylase 7 in Human [ChEMBL: CHEMBL2716] [GtoPdb: 2661] [UniProtKB: Q8WUI4] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase 8 in Schistosoma mansoni [ChEMBL: CHEMBL3797017] [UniProtKB: A5H660] histone deacetylase 8/Histone deacetylase 8 in Human [ChEMBL: CHEMBL3192] [GtoPdb: 2619] [UniProtKB: Q9BY41] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 9/Histone deacetylase 9 in Human [ChEMBL: CHEMBL4145] [GtoPdb: 2620] [UniProtKB: Q9UKV0] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase HD1B in Zea mays [ChEMBL: CHEMBL3541] [UniProtKB: Q9ZTP8] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase HD2 in Zea mays [ChEMBL: CHEMBL4919] [UniProtKB: Q94F81] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase-like amidohydrolase in Alcaligenes sp. (strain DSM 11172) (Bordetella sp. (strain FB188)) [ChEMBL: CHEMBL6017] [UniProtKB: Q70I53] There should be some charts here, you may need to enable JavaScript!
euchromatic histone lysine methyltransferase 2/Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 in Human [ChEMBL: CHEMBL6032] [GtoPdb: 2652] [UniProtKB: Q96KQ7] There should be some charts here, you may need to enable JavaScript!
hydroxymethylglutaryl-CoA reductase/HMG-CoA reductase in Human [ChEMBL: CHEMBL402] [GtoPdb: 639] [UniProtKB: P04035] There should be some charts here, you may need to enable JavaScript!
Human immunodeficiency virus type 1 Tat protein in Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)(HIV-1) [ChEMBL: CHEMBL4011] [UniProtKB: P04608] There should be some charts here, you may need to enable JavaScript!
Leukotriene A₄ hydrolase/Leukotriene A4 hydrolase in Human [ChEMBL: CHEMBL4618] [GtoPdb: 1395] [UniProtKB: P09960] Leukotriene A₄ hydrolase/Leukotriene A4 hydrolase in Mouse [ChEMBL: CHEMBL3738] [GtoPdb: 1395] [UniProtKB: P24527] There should be some charts here, you may need to enable JavaScript!
lysine demethylase 4E/Lysine-specific demethylase 4D-like in Human [ChEMBL: CHEMBL1293226] [GtoPdb: 2679] [UniProtKB: B2RXH2] There should be some charts here, you may need to enable JavaScript!
lysine demethylase 1A/Lysine-specific histone demethylase 1 in Human [ChEMBL: CHEMBL6136] [GtoPdb: 2669] [UniProtKB: O60341] There should be some charts here, you may need to enable JavaScript!
Monoamine oxidase A in Human [ChEMBL: CHEMBL1951] [GtoPdb: 2489] [UniProtKB: P21397] There should be some charts here, you may need to enable JavaScript!
Monoamine oxidase B in Human [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338] There should be some charts here, you may need to enable JavaScript!
sirtuin 5/NAD-dependent protein deacylase sirtuin-5, mitochondrial in Human [ChEMBL: CHEMBL2163183] [GtoPdb: 2711] [UniProtKB: Q9NXA8] There should be some charts here, you may need to enable JavaScript!
MDM2 proto-oncogene/p53-binding protein Mdm-2 in Human [ChEMBL: CHEMBL5023] [GtoPdb: 3136] [UniProtKB: Q00987] There should be some charts here, you may need to enable JavaScript!
phosphoinositide-3-kinase regulatory subunit 1/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha/p85-alpha in Human [ChEMBL: CHEMBL2111367] [GtoPdb: 2503, 2153] [UniProtKB: P27986, P42336] There should be some charts here, you may need to enable JavaScript!
Plasmodium berghei [ChEMBL: CHEMBL612653] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium vivax [ChEMBL: CHEMBL613013] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 5/Proteasome Macropain subunit MB1 in Human [ChEMBL: CHEMBL4662] [GtoPdb: 2406] [UniProtKB: P28074] There should be some charts here, you may need to enable JavaScript!
ABL proto-oncogene 1, non-receptor tyrosine kinase/Tyrosine-protein kinase ABL in Human [ChEMBL: CHEMBL1862] [GtoPdb: 1923] [UniProtKB: P00519] There should be some charts here, you may need to enable JavaScript!
Janus kinase 2/Tyrosine-protein kinase JAK2 in Human [ChEMBL: CHEMBL2971] [GtoPdb: 2048] [UniProtKB: O60674] There should be some charts here, you may need to enable JavaScript!
fms related receptor tyrosine kinase 3/Tyrosine-protein kinase receptor FLT3 in Human [ChEMBL: CHEMBL1974] [GtoPdb: 1807] [UniProtKB: P36888] There should be some charts here, you may need to enable JavaScript!
kinase insert domain receptor/Vascular endothelial growth factor receptor 2 in Human [ChEMBL: CHEMBL279] [GtoPdb: 1813] [UniProtKB: P35968] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Amyloid-beta A4 protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2487] [UniProtKB: P05067]
ChEMBL	Inhibition of amyloid beta (1 to 42) (unknown origin) self aggregation after 24 hrs by thioflavin-T fluorescence method	B	4.7	pIC50	>20000	nM	IC50	Eur J Med Chem (2020) 192: 112193-112193 [PMID:32151835]
Apoptosis regulator BAX in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5318] [UniProtKB: Q07812]
ChEMBL	Binding affinity to recombinant human full length His-tagged BAX expressed in Escherichia coli BL21 (DE3) cells preincubated for 30 min followed by FITC-BIM addition and measured after 20 mins by fluorescence polarization assay	B	5	pEC50	>10000	nM	EC50	J Med Chem (2020) 63: 12083-12099 [PMID:33021789]
bromodomain containing 4/Bromodomain-containing protein 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885]
ChEMBL	Inhibition of recombinant BRD4 bromodomain 1 (unknown origin) using histone H4 peptide as substrate incubated for 1 hr under dark condition by HTRF assay	B	4.3	pIC50	>50000	nM	IC50	Medchemcomm (2014) 5: 342-351
ChEMBL	Inhibition of His-tagged BRD4 bromodomain 1 (unknown origin) using [Lys (5,8,12,16) Ac] H4(1-21) as substrate by fluorescence based assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
ChEMBL	Inhibition of His-tagged BRD4 bromodomain 2 (unknown origin) using [Lys (5,8,12,16) Ac] H4(1-21) as substrate by fluorescence based assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
ChEMBL	Inhibition of BRD4 in human HL60 cells assessed as reduction in C-myc production after 24 hrs by ELISA	B	6.13	pIC50	740	nM	IC50	Bioorg Med Chem (2017) 25: 3677-3684 [PMID:28549889]
ChEMBL	Inhibition of BRD4 (unknown origin) by ELISA	B	7.37	pIC50	43.13	nM	IC50	Bioorg Med Chem Lett (2021) 31: 127671-127671 [PMID:33229136]
ChEMBL	Inhibition of BRD4 (unknown origin)	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4051-4055 [PMID:28765013]
DNA methyltransferase 1/DNA (cytosine-5)-methyltransferase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1993] [GtoPdb: 2605] [UniProtKB: P26358]
ChEMBL	Inhibition of human DNMT1 enzyme using polydeoxy-inosine polydeoxy-cytosine DNA as substrate incubated for 15 mins in presence of SAM by TR-FRET assay	B	5	pIC50	>10000	nM	IC50	J Med Chem (2021) 64: 3392-3426 [PMID:33661013]
epidermal growth factor receptor/Epidermal growth factor receptor erbB1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL203] [GtoPdb: 1797] [UniProtKB: P00533]
ChEMBL	Inhibition of EGFR (unknown origin)	B	6.05	pIC50	900	nM	IC50	J Med Chem (2020) 63: 8977-9002 [PMID:32320239]
ChEMBL	Inhibition of EGFR (unknown origin)	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
histone deacetylase 1/HDAC1/REST corepressor 3 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4296112] [GtoPdb: 2658] [UniProtKB: Q13547, Q9P2K3]
ChEMBL	Inhibition of HDAC1/CoREST3 in HEK293 whole cell extract using fluorescent acetylated histone peptide as substrate after 60 mins by fluorescence based assay	B	8.52	pKi	3.04	nM	Ki	Bioorg Med Chem Lett (2018) 28: 1001-1004 [PMID:29496367]
GtoPdb	-	-	8.89	pKi	1.3	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
heat shock protein 90 alpha family class A member 1/Heat shock protein HSP 90-alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3880] [GtoPdb: 2905] [UniProtKB: P07900]
ChEMBL	Inhibition of FITC-geldanamycin binding to HSP90alpha (unknown origin) after 3 hrs by fluorescence polarization assay	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2018) 150: 667-677 [PMID:29567459]
ChEMBL	Inhibition of HSP90a (unknown origin)	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2020) 190: 112086-112086 [PMID:32058238]
K_v11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL	Inhibition of human ERG by automated patch clamp assay	B	4.52	pIC50	>30000	nM	IC50	Bioorg Med Chem Lett (2011) 21: 4909-4912 [PMID:21742496]
ChEMBL	Inhibition of human ERG by automated Q-patch assay	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2011) 54: 4752-4772 [PMID:21650221]
ChEMBL	Inhibition of recombinant human ERG expressed in HEK293 cells at -80 mV holding potential measured for 9 mins by Qpatch clamp method	B	4.52	pIC50	>30000	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4885-4888 [PMID:28947154]
ChEMBL	Inhibition of human ERG	B	6.49	pIC50	322	nM	IC50	Eur J Med Chem (2020) 206: 112677-112677 [PMID:32823005]
Histone deacetylase in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5541] [UniProtKB: Q9XYC7]
ChEMBL	Inhibition of Plasmodium falciparum HDAC1	B	6.89	pIC50	130	nM	IC50	J Med Chem (2010) 53: 6100-6111 [PMID:20669972]
ChEMBL	Inhibition of Plasmodium falciparum HDAC1 expressed in Drosophila melanogaster S2 cells	B	7.23	pIC50	59	nM	IC50	J Med Chem (2009) 52: 2185-2187 [PMID:19317450]
Plasmodium falciparum histone deacetylase 1/Histone deacetylase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293190] [GtoPdb: 3072] [UniProtKB: Q7K6A1]
ChEMBL	Inhibition of Plasmodium falciparum 3D7 histone deacetylase infected in human erythrocytes assessed as alteration of histone H3 acetylation after 3.5 hrs by SDS-PAGE	B	7	pIC50	100	nM	IC50	Antimicrob Agents Chemother (2008) 52: 3467-3477 [PMID:18644969]
histone deacetylase 1/Histone deacetylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547]
ChEMBL	Binding affinity to human HDAC1 assessed as dissociation constant	B	6.74	pKd	182	nM	Kd	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of recombinant human HDAC1 using Boc-L-Lys(acetyl)-MCA as substrate by fluorogenic enzymatic assay	B	7.4	pKd	40	nM	Kd	Bioorg Med Chem (2012) 20: 985-995 [PMID:22182579]
ChEMBL	Inhibition of HDAC1	B	7.2	pKi	63	nM	Ki	Bioorg Med Chem Lett (2010) 20: 3314-3321 [PMID:20451378]
ChEMBL	Competitive inhibition of HDAC1 using KI-104 as substrate by fluorescence assay	B	7.22	pKi	60	nM	Ki	J Med Chem (2011) 54: 4694-4720 [PMID:21634430]
ChEMBL	Inhibition of HDAC1	B	7.32	pKi	48	nM	Ki	J Med Chem (2008) 51: 2898-2906 [PMID:18412327]
ChEMBL	Inhibition of human recombinant HDAC1 using Fluor de Lys as substrate after 2 hrs by fluorescence method	B	7.92	pKi	12	nM	Ki	Bioorg Med Chem Lett (2018) 28: 2985-2992 [PMID:30122227]
ChEMBL	Inhibition of human recombinant HDAC1 using Ac-Leu-Gly-Lys (Ac)-AMC substrate incubated for 30 mins by fluorescence assay	B	8	pKi	10	nM	Ki	Medchemcomm (2014) 5: 1849-1855
ChEMBL	Inhibition of recombinant human HDAC1 enzyme using flurogenic Ac-LeuGlyLys (Ac)-AMC as substrate after 15 to 30 mins	B	8.1	pKi	8	nM	Ki	J Med Chem (2013) 56: 6512-6520 [PMID:23865683]
ChEMBL	Inhibition of human recombinant HDAC1 using [Ac-Leu-Gly-Lys(Ac)-AMC substrate incubated for 15 to 30 mins	B	8.1	pKi	8	nM	Ki	J Med Chem (2014) 57: 9644-9657 [PMID:25380299]
ChEMBL	Inhibition of recombinant His-tagged human HDAC1 expressed in insect cells assessed as hydrolase activity using fluorogenic Ac-LGK-ac-AMC as substrate measured after 60 mins by fluorescence analysis	B	8.19	pKi	6.4	nM	Ki	Medchemcomm (2016) 7: 464-470
ChEMBL	Binding affinity to recombinant human HDAC1 expressed in baculovirus infected HEK293T cells using acetylLys(Ac)-AMC as substrate assessed as inhibition constant	B	8.27	pKi	5.4	nM	Ki	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in sf9 cells using acetyl-Lys(Ac)-AMC as substrate	B	8.3	pKi	5	nM	Ki	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Binding affinity to recombinant human HDAC1 assessed as inhibition constant	B	8.72	pKi	1.9	nM	Ki	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of human HDAC1	B	8.89	pKi	1.3	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
GtoPdb	-	-	8.89	pKi	1.3	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) incubated for 3 hrs by chemiluminescent assay	B	4.48	pIC50	33000	nM	IC50	Eur J Med Chem (2022) 244: 114807-114807 [PMID:36244186]
ChEMBL	Inhibition of HDAC1 in human COLO205 cells assessed as induction of histone H3 acetylation	B	5.66	pIC50	2200	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3314-3321 [PMID:20451378]
ChEMBL	Inhibition of HDAC1 in presence of DTT	B	6.11	pIC50	775	nM	IC50	J Med Chem (2007) 50: 5720-5726 [PMID:17958342]
ChEMBL	Inhibition of HDAC1 in human 293T cells after 16 hrs by fluorimetry	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2009) 19: 336-340 [PMID:19084395]
ChEMBL	Inhibition of human recombinant HDAC1 using modified Boc-Lys(Ac)-AMC MAL as substrate after 1 hr by fluorescent activity assay	B	6.22	pIC50	600	nM	IC50	ACS Med Chem Lett (2014) 5: 973-978 [PMID:25221651]
ChEMBL	Inhibition of Histone deacetylase 1 (HDAC1) of HeLa nuclear extracts	B	6.32	pIC50	480	nM	IC50	Bioorg Med Chem Lett (2004) 14: 449-453 [PMID:14698179]
ChEMBL	Inhibition of human recombinant HDAC1 by fluorimetry	B	6.46	pIC50	350	nM	IC50	J Med Chem (2009) 52: 2909-2922 [PMID:19419205]
ChEMBL	Inhibition of HDAC1 (unknown origin) by fluorimetric assay	B	6.46	pIC50	350	nM	IC50	Bioorg Med Chem Lett (2016) 26: 154-159 [PMID:26611919]
ChEMBL	Inhibition of human HDAC1 by fluorescence assay	B	6.49	pIC50	320	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of full length His-tagged human recombinant HDAC1 expressed in baculovirus system using fluor de Lys as substrate by fluorometric analysis	B	6.51	pIC50	310	nM	IC50	Eur J Med Chem (2013) 66: 56-68 [PMID:23792316]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	6.51	pIC50	310	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic peptide as substrate by fluorescence assay	B	6.51	pIC50	310	nM	IC50	J Med Chem (2014) 57: 8026-8034 [PMID:25238284]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic peptide from p53 residues (379 to 382) (RHKK(Ac)) as substrate by fluorescence assay	B	6.51	pIC50	306	nM	IC50	ACS Med Chem Lett (2013) 4: 779-783 [PMID:24015327]
ChEMBL	Inhibition of C-terminal FLAG His tagged full length human recombinant HDAC1 (1 to 482 residues) expressed in baculovirus infected sf21 cells using RHKKAc as substrate in presence of ATP	B	6.51	pIC50	306	nM	IC50	J Med Chem (2017) 60: 8336-8357 [PMID:28953386]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Arg-His-Lys-Lys(Ac) as substrate after 2 hrs by fluorescence analysis	B	6.51	pIC50	306	nM	IC50	Bioorg Med Chem Lett (2018) 28: 1357-1362 [PMID:29545103]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.51	pIC50	306	nM	IC50	Eur J Med Chem (2018) 158: 593-619 [PMID:30243158]
ChEMBL	Inhibition of human HDAC1 using Arg-His-Lys-Lys (Ac) as substrate incubated for 2 hrs by fluorescence assay	B	6.51	pIC50	306	nM	IC50	Eur J Med Chem (2019) 184: 111755-111755 [PMID:31627059]
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in sf21 cells preincubated with enzyme followed by fluorogenic Arg-His-Lys-Lys(Ac)-AMC substrate addition measured after 2 hrs by fluorescence assay	B	6.51	pIC50	306	nM	IC50	J Med Chem (2016) 59: 8233-8262 [PMID:27541357]
ChEMBL	Inhibition of human recombinant HDAC1 using histone H3 substrate pre-incubated at room temperature for 15 mins before substrate addition by fluorimetric assay	B	6.52	pIC50	300	nM	IC50	J Med Chem (2014) 57: 6259-6265 [PMID:24972008]
ChEMBL	Inhibition of HDAC1 by fluorescent activity assay	B	6.54	pIC50	290	nM	IC50	Bioorg Med Chem Lett (2011) 21: 5735-5738 [PMID:21875796]
ChEMBL	Inhibition of HDAC1 by fluorometric assay	B	6.57	pIC50	270	nM	IC50	J Med Chem (2012) 55: 9562-9575 [PMID:23116147]
ChEMBL	Inhibition of human recombinant HDAC1 using AMC-K(Ac)GL as substrate by fluorescence based assay	B	6.59	pIC50	260	nM	IC50	ACS Med Chem Lett (2020) 11: 977-983 [PMID:32435414]
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG-His-tagged HDAC1 (1 to 482 residues) expressed in sf21 cells using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	6.59	pIC50	260	nM	IC50	Eur J Med Chem (2016) 112: 99-105 [PMID:26890116]
ChEMBL	Inhibition of human HDAC-1 using RHKK(Ac) as substrate by fluorescence assay	B	6.59	pIC50	260	nM	IC50	J Med Chem (2014) 57: 2258-2274 [PMID:24588105]
ChEMBL	Inhibition of human HDAC1 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.59	pIC50	258	nM	IC50	Bioorg Med Chem Lett (2014) 24: 61-64 [PMID:24345446]
ChEMBL	Inhibition of human HDAC1 using fluorogenic tetrapeptide RHKKAc as substrate	B	6.59	pIC50	258	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic tetrapeptide RHKK(Ac) substrate by fluorescence assay	B	6.59	pIC50	258	nM	IC50	J Med Chem (2014) 57: 8358-8377 [PMID:25233084]
ChEMBL	Inhibition of human HDAC-1 using RHKK(Ac) as substrate	B	6.59	pIC50	258	nM	IC50	Eur J Med Chem (2014) 76: 53-60 [PMID:24565573]
ChEMBL	Inhibition of human HDAC1 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.59	pIC50	258	nM	IC50	Bioorg Med Chem Lett (2015) 25: 459-461 [PMID:25563890]
ChEMBL	Inhibition of human HDAC1 by fluorimetric assay	B	6.59	pIC50	258	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of Nanoluc-fused HDAC1 (unknown origin) expressed in HEK293 cells incubated for 2 hrs by NANOBRET assay	B	6.62	pIC50	240	nM	IC50	J Med Chem (2021) 64: 4810-4840 [PMID:33830764]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKK(Ac)AMC as substrate	B	6.62	pIC50	240	nM	IC50	J Med Chem (2015) 58: 2809-2820 [PMID:25734520]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic HDAC substrate-3 by fluorescence assay	B	6.63	pIC50	236	nM	IC50	Eur J Med Chem (2019) 164: 263-272 [PMID:30597327]
ChEMBL	Inhibition of recombinant human HDAC1	B	6.66	pIC50	220	nM	IC50	Bioorg Med Chem Lett (2016) 26: 375-379 [PMID:26706171]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic substrate incubated for 30 mins by fluorescence plate reader	B	6.66	pIC50	219.3	nM	IC50	Eur J Med Chem (2022) 240: 114582-114582 [PMID:35834905]
ChEMBL	Inhibition of full length His6-tagged GST-fused recombinant human HDAC1 expressed in High5 insect cells using Ac-Lys-Tyr-Lys (e-acetyl)-AMC as substrate after 24 hrs by fluorescence assay	B	6.68	pIC50	210.98	nM	IC50	Eur J Med Chem (2016) 112: 81-90 [PMID:26890114]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys (acetyl)-AMC as substrate preincubated for 30 mins before substrate addition measured after 20 mins by UV-vis spectrophotometer analysis	B	6.69	pIC50	203.06	nM	IC50	Bioorg Med Chem (2015) 23: 4728-4736 [PMID:26100440]
ChEMBL	Inhibitory activity against human Histone deacetylase 1	B	6.7	pIC50	200	nM	IC50	J Med Chem (2004) 47: 3409-3417 [PMID:15189037]
ChEMBL	Inhibition of human HDAC1	B	6.7	pIC50	200	nM	IC50	J Med Chem (2008) 51: 1505-1529 [PMID:18247554]
ChEMBL	Inhibition of HDAC1 by in vitro deacetylation assay	B	6.7	pIC50	200	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of C-terminal His-tagged and C-terminal FLAG-tagged full length human recombinant HDAC1 expressed in baculovirus expression system using fluorogenic acetylated peptide as substrate preincubated with enzyme for 10 mins prior to substrate addition measured after 60 mins by fluorescence analysis	B	6.71	pIC50	196	nM	IC50	ACS Med Chem Lett (2013) 4: 132-136 [PMID:24900575]
ChEMBL	Inhibition of full length recombinant human GST fused His6-tagged HDAC1 expressed in baculovirus infected High5 cells using Ac-Lys-Tyr-Lys(epsilon-acetyl)-AMC as substrate measured after 24 hrs	B	6.71	pIC50	195	nM	IC50	Eur J Med Chem (2017) 128: 293-299 [PMID:28213282]
ChEMBL	Inhibition of HDAC1 in human HeLa cell nuclear extract using Boc-Lys (Ac)-AMC as substrate after 60 mins by fluorometric analysis	B	6.72	pIC50	190	nM	IC50	J Med Chem (2015) 58: 9214-9227 [PMID:26555243]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in baculovirus infected High5 insect cells using Ac-Lys-Tyr-Lys(e-acetyl)-AMC as substrate incubated for 24 hrs measured after 30 mins by microplate reader based spectrophotometric analysis	B	6.72	pIC50	190	nM	IC50	Bioorg Med Chem (2020) 28: 115639-115639 [PMID:32773090]
ChEMBL	Inhibition of human recombinant HDAC1 using Fluor de Lys-Green as substrate preincubated for 5 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	6.72	pIC50	190	nM	IC50	Eur J Med Chem (2018) 143: 320-333 [PMID:29202397]
ChEMBL	Inhibition of recombinant human HDAC1 (482 residues) expressed in baculovirus infected insect cells preincubated with enzyme followed by flour de lys-green substrate addition measured after 1 hr by fluorescence assay	B	6.72	pIC50	190	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4415-4420 [PMID:28818449]
ChEMBL	Inhibition of HDAC1 (unknown origin) incubated for 60 mins using microplate reader analysis	B	6.74	pIC50	180.3	nM	IC50	J Med Chem (2022) 65: 1243-1264 [PMID:33586434]
ChEMBL	Inhibition of human recombinant His6-tagged and GST-fuses HDAC1 expressed in insect High5 cells using Ac-Lys-Tyr-Lys(e-acetyl)-AMC as substrate after 24 hrs by fluorescence assay	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4540-4545 [PMID:22738629]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem Lett (2021) 31: 127671-127671 [PMID:33229136]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem Lett (2021) 38: 127829-127829 [PMID:33685790]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.74	pIC50	180	nM	IC50	Medchemcomm (2014) 5: 342-351
ChEMBL	Inhibition of HDAC1 in human HeLa cell nuclear extract using Bos-Lys(acetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition incubated for 30 mins	B	6.75	pIC50	178	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of HDAC1	B	6.77	pIC50	170	nM	IC50	Eur J Med Chem (2010) 45: 4331-4338 [PMID:20637529]
ChEMBL	Inhibition of recombinant human HDAC1 using Z-(Ac)-Lys-AMC as substrate incubated for 40 mins by fluorescence based assay	B	6.79	pIC50	162.7	nM	IC50	Eur J Med Chem (2021) 218: 113392-113392 [PMID:33831778]
ChEMBL	Inhibition of HDAC 1 in human HeLa nuclear extract using HDAC substrate-3 measured after 60 mins by fluorometric analysis	B	6.82	pIC50	150	nM	IC50	J Med Chem (2017) 60: 3484-3497 [PMID:28368585]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) using fluorogenic substrate Boc-Lys (acetyl)-AMC after 20 mins by homogeneous fluorescence release assay	B	6.82	pIC50	150	nM	IC50	Eur J Med Chem (2015) 96: 1-13 [PMID:25874326]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) incubated for 10 mins using Boc-Lys(acetyl)-AMC fluorogenic substrate by homogeneous fluorescence release assay	B	6.82	pIC50	150	nM	IC50	Bioorg Med Chem (2015) 23: 3457-3471 [PMID:25953722]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys (Ac)-AMC as substrate incubated for 60 mins and measured after 30 mins by fluorescence assay	B	6.85	pIC50	142	nM	IC50	Eur J Med Chem (2019) 168: 515-526 [PMID:30851694]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.85	pIC50	142	nM	IC50	Eur J Med Chem (2018) 144: 398-409 [PMID:29288941]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys (Ac)-AMC as substrate incubated for 60 mins by fluorimetric assay	B	6.85	pIC50	142	nM	IC50	J Nat Prod (2019) 82: 1442-1450 [PMID:31120744]
ChEMBL	Inhibition of human recombinant HDAC1 using Tosyl-Gly-ProLys(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	6.85	pIC50	140	nM	IC50	J Med Chem (2021) 64: 10403-10417 [PMID:34185525]
ChEMBL	Inhibition of recombinant C-terminal FLAG-tagged HDAC1 expressed in baculovirus after 10 mins by fluorimetric analysis	B	6.85	pIC50	140	nM	IC50	Bioorg Med Chem Lett (2009) 19: 5684-5688 [PMID:19699639]
ChEMBL	Inhibition of human HDAC1	B	6.85	pIC50	140	nM	IC50	J Med Chem (2008) 51: 7417-7427 [PMID:19007204]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.86	pIC50	138.5	nM	IC50	Eur J Med Chem (2021) 226: 113870-113870 [PMID:34610548]
ChEMBL	Inhibition of recombinant human HDAC1 using Boc-Lys(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence-based assay	B	6.87	pIC50	136	nM	IC50	Eur J Med Chem (2016) 109: 350-359 [PMID:26814680]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic substrate measured after 30 mins	B	6.88	pIC50	132	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic substrate measured after 30 mins	B	6.88	pIC50	131.58	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of human recombinant HDAC1 after 60 mins by fluorimetric assay	B	6.88	pIC50	131	nM	IC50	Eur J Med Chem (2014) 86: 639-652 [PMID:25218912]
ChEMBL	Inhibition of human recombinant HDAC1 incubated for 10 mins prior to substrate addition measured after 60 mins by spectrophotometric analysis	B	6.88	pIC50	131	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3295-3299 [PMID:23601706]
ChEMBL	Inhibition of C-terminal 6His/FLAG tagged human recombinant HDAC1 (1 to 482 residues) purified as tubulin complex expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate preincubated for 60 mins followed by substrate addition and measured after 90 mins by microplate reader analysis	B	6.89	pIC50	128	nM	IC50	J Med Chem (2023) 66: 13821-13837 [PMID:37782298]
ChEMBL	Inhibition of HDAC activity measured by HDAC Fluorescent Activity Assay (mean of two experiments)	F	6.9	pIC50	125	nM	IC50	Bioorg Med Chem Lett (2007) 17: 370-375 [PMID:17095213]
ChEMBL	Inhibition of HDAC activity measured by HDAC Fluorescent Activity Assay (mean of two experiments)	F	6.9	pIC50	125	nM	IC50	Bioorg Med Chem Lett (2007) 17: 363-369 [PMID:17107790]
ChEMBL	Inhibition of HDAC1 (unknown origin) expressed in HEK293 cells cotransfected with nano-luciferase incubated for 2 hrs followed by NanoBRET NanoGlo Substrate addition by NanoBRET assay	B	6.91	pIC50	122	nM	IC50	J Med Chem (2019) 62: 8557-8577 [PMID:31414801]
ChEMBL	Inhibition of HDAC1	B	6.92	pIC50	121	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3314-3321 [PMID:20451378]
ChEMBL	Inhibition of human recombinant HDAC1 using ZMAL (Z-Lys(Ac)-AMC) fluorogenic substrate incubated for 90 mins by fluorescence based assay	B	6.92	pIC50	120	nM	IC50	J Med Chem (2020) 63: 10339-10351 [PMID:32803970]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in High5 insect cells using Ac-Lys-Tyr-Lys(-acetyl)-AMC as substrate after 24 hrs by fluorescence assay	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem (2013) 21: 3240-3244 [PMID:23602523]
ChEMBL	Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate reader	B	6.92	pIC50	120	nM	IC50	J Med Chem (2021) 64: 14620-14646 [PMID:34582215]
ChEMBL	Inhibition of recombinant HDAC1 by fluorimetric assay	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2009) 19: 1403-1408 [PMID:19181524]
ChEMBL	Inhibition of full length C-terminal 6His/FLAG-tagged human recombinant HDAC1 (1 to 482 residues) purified as tubulin complex expressed in Sf9 cells using ZMAL as fluorogenic substrate incubated for 90 mins by microplate reader analysis	B	6.92	pIC50	120	nM	IC50	J Med Chem (2023) 66: 14787-14814 [PMID:37902787]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4457-4460 [PMID:26376355]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in baculovirus infected insect High5 cells using Ac-Lys-Tyr-Lys(epsilon-acetyl)-AMC as substrate by fluorescence assay	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2011) 21: 4844-4846 [PMID:21745740]
ChEMBL	Inhibition of human recombinant HDAC1 after 15 mins by fluorimetric assay	B	6.92	pIC50	120	nM	IC50	J Med Chem (2010) 53: 4654-4667 [PMID:20491440]
ChEMBL	Inhibition of human recombinant HDAC1 using ZMAL (Z-(Ac)Lys-AMC fluorogenic substrate incubated for 90 mins by fluorescence method	B	6.92	pIC50	119.5	nM	IC50	ACS Med Chem Lett (2019) 10: 671-676 [PMID:30996816]
ChEMBL	Inhibition of full length GST-tagged human HDAC1 using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	6.92	pIC50	119	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Inhibition of full length recombinant HDAC1 using Fluor de Lys as substrate by fluorescence assay	B	6.92	pIC50	119	nM	IC50	J Med Chem (2011) 54: 4694-4720 [PMID:21634430]
ChEMBL	Inhibition of full length FLAG-tagged recombinant HDAC1 (unknown origin) transfected in human HeLa cells assessed as deacetylation of [3H]-acetylated histones after 2 hrs by scintillation counting analysis	B	6.92	pIC50	119	nM	IC50	J Med Chem (2013) 56: 7201-7211 [PMID:23964961]
ChEMBL	Inhibition of human HDAC1 using (Z-(Ac)Lys-AMC) as substrate after 90 mins by fluorescence analysis	B	6.92	pIC50	119	nM	IC50	J Med Chem (2016) 59: 1545-1555 [PMID:26653328]
ChEMBL	Ex vivo inhibition of human HDAC1 in human Caco-2 cells by fluorometric cellular activity assay	B	6.92	pIC50	119	nM	IC50	J Med Chem (2010) 53: 1937-1950 [PMID:20143840]
ChEMBL	Inhibition of recombinant human HDAC1 using Cbz-(Ac)Lys-AMC as substrate preincubated for 90 mins followed by trypsin addition measured after 20 mins by fluorescence assay	B	6.93	pIC50	117	nM	IC50	J Med Chem (2016) 59: 2423-2435 [PMID:26937828]
ChEMBL	Inhibition of recombinant C-terminal His/FLAG-tagged human HDAC1 expressed in baculovirus expression system using fluorogenic ZMAL as substrate incubated for 90 mins by fluorescence assay	B	6.93	pIC50	117	nM	IC50	J Med Chem (2019) 62: 1138-1166 [PMID:30645113]
ChEMBL	Inhibition of recombinant human full length C-terminal His/FLAG-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells using ZMAL as substrate incubated for 90 mins by fluorescence assay	B	6.95	pIC50	111	nM	IC50	J Med Chem (2019) 62: 11260-11279 [PMID:31762274]
ChEMBL	Inhibition of recombinant human FLAG/His-tagged HDAC1 expressed in baculovirus expression system using 7-AMC-labelled HDAC substrate measured after 30 mins by fluorescence assay	B	6.96	pIC50	110	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC1 by fluorometric assay	B	6.96	pIC50	110	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6200-6204 [PMID:22932316]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKK(Ac) (379 to 382) p53 peptide as substrate by fluorescence assay	B	6.96	pIC50	110	nM	IC50	J Med Chem (2014) 57: 4009-4022 [PMID:24766560]
ChEMBL	Inhibition of C-terminal His/FLAG-tagged full length recombinant human HDAC1 expressed in baculovirus expression system assessed as release of 7-amino-4-methylcoumarin by fluorogenic assay	B	6.96	pIC50	110	nM	IC50	Eur J Med Chem (2016) 122: 92-101 [PMID:27344487]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.96	pIC50	110	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of recombinant human full length HDAC1 using Fluor-de-Lys as substrate after 60 mins by spectrofluorimetric analysis	B	6.96	pIC50	110	nM	IC50	Eur J Med Chem (2017) 134: 13-23 [PMID:28395150]
ChEMBL	Inhibition of human HDAC1 using Boc-Lys (acetyl)-AMC as substrate pretreated with compound for 1 hrs followed by substrate addition measured after 1 hrs by microplate reader analysis	B	6.97	pIC50	106.13	nM	IC50	Eur J Med Chem (2022) 243: 114705-114705 [PMID:36215854]
ChEMBL	Inhibition of HDAC1 (unknown origin) using substrate after 30 mins by fluorometric analysis	B	6.97	pIC50	106	nM	IC50	Eur J Med Chem (2017) 133: 11-23 [PMID:28371677]
ChEMBL	Inhibition of C-terminal 6His tagged human recombinant full length HDAC1 (1 to 482 residues) using Z- Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins followed by trypsin addition and measured after 30 min by microplate reader analysis	B	6.99	pIC50	102.8	nM	IC50	ACS Med Chem Lett (2023) 14: 1863-1868 [PMID:38116436]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 2 hrs by fluorescence assay	B	6.99	pIC50	102	nM	IC50	Bioorg Med Chem (2023) 85: 117242-117242 [PMID:37079967]
ChEMBL	Inhibition of recombinant human HDAC1 using fluorogenic substrate by fluorescence assay	B	6.99	pIC50	101.98	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of recombinant full length C-terminal FLAG/His-tagged human HDAC1 expressed in baculovirus infected Sf9 cells using Z-Lys(Ac)-AMC as substrate incubated for 90 mins followed by trypsin addition and measured after 30 mins by fluorescence based assay	B	7	pIC50	101	nM	IC50	Medchemcomm (2019) 10: 1109-1115 [PMID:31391882]
ChEMBL	Inhibition of human recombinant C-terminal FLAG-tagged HDAC1 expressed in baculovirus by fluorimetry	B	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2009) 19: 336-340 [PMID:19084395]
ChEMBL	Inhibition of recombinant human HDAC1 expressed in baculovirus infected High5 insect cells using Ac-Lys-Tyr-Lys(epsilon-acetyl)-AMC as substrate after 24 hrs by fluorescence assay	B	7	pIC50	100	nM	IC50	Bioorg Med Chem (2018) 26: 4706-4715 [PMID:30115492]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis	B	7	pIC50	99	nM	IC50	J Med Chem (2022) 65: 15457-15472 [PMID:36351184]
ChEMBL	Inhibition of recombinant human His-tagged HDAC1 (482 residues) expressed in baculovirus infected insect cells using Boc Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	7.01	pIC50	98.13	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC1 in human HeLa cells using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by fluorescence based analysis	B	7.01	pIC50	98.13	nM	IC50	Bioorg Med Chem (2021) 52: 116510-116510 [PMID:34826681]
ChEMBL	Inhibition of HDAC1 after 30 mins by Fluor de Lys fluorescence assay	B	7.02	pIC50	96	nM	IC50	Bioorg Med Chem Lett (2011) 21: 6139-6142 [PMID:21889343]
ChEMBL	Inhibition of HDAC1 after 17 hrs	B	7.02	pIC50	96	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of human recombinant HDAC1	B	7.02	pIC50	96	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3023-3026 [PMID:19419863]
ChEMBL	Inhibition of HDAC1 using Fluor-de-Lys as substrate compound pretreated for 30 mins before substrate addition by fluorescence assay	B	7.02	pIC50	96	nM	IC50	Bioorg Med Chem Lett (2012) 22: 7084-7086 [PMID:23089527]
ChEMBL	Inhibition of HDAC1 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	7.02	pIC50	96	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as fluorogenic substrate measured after 1 hr by flourescence plate reader method	B	7.03	pIC50	93.6	nM	IC50	Eur J Med Chem (2021) 225: 113799-113799 [PMID:34500130]
ChEMBL	Inhibition of recombinant His6-tagged GST-fused human HDAC1 expressed in baculovirus infected insect High5 cells using Ac-Lys-Tyr-Lys(epsilon-acetyl)-AMC as substrate after 24 hrs	B	7.03	pIC50	93.34	nM	IC50	Bioorg Med Chem (2015) 23: 5881-5890 [PMID:26211462]
ChEMBL	Inhibition of human recombinant full length HDAC1 using (Ac)-Lys-Tyr-Lys(-acetyl)-AMC substrate after 30 mins	B	7.03	pIC50	93.34	nM	IC50	Bioorg Med Chem (2014) 22: 6146-6155 [PMID:25261927]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition by fluorescence assay	B	7.04	pIC50	91	nM	IC50	Medchemcomm (2015) 6: 1816-1825
ChEMBL	Inhibition of human recombinant HDAC1 expressed in baculovirus infected insect high5 cells using Ac-Lys-Tyr-Lys (epsilon-acetyl)-AMC as substrate after 3 to 24 hrs by fluorescence assay	B	7.05	pIC50	90	nM	IC50	Bioorg Med Chem (2013) 21: 6981-6995 [PMID:24095018]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) using Fluor-de-Lys-SIRT1 as substrate by fluorescence assay	B	7.05	pIC50	90	nM	IC50	J Nat Prod (2014) 77: 49-56 [PMID:24328302]
ChEMBL	Inhibition of recombinant his-tagged human HDAC1 using Ac-LeuGlyLys (Ac) AMC substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate reader analysis	B	7.05	pIC50	89	nM	IC50	J Med Chem (2022) 65: 12200-12218 [PMID:36097406]
ChEMBL	Inhibition of recombinant human full length C-terminal Flag/His-tagged HDAC1 expressed in baculovirus infected Sf9 cells using ZMAL as substrate incubated for 90 mins by fluorometric method	B	7.05	pIC50	89	nM	IC50	Bioorg Med Chem (2019) 27: 115039-115039 [PMID:31420257]
ChEMBL	Inhibition of recombinant human HDAC1 after 60 mins by fluorescence assay	B	7.05	pIC50	88.5	nM	IC50	J Med Chem (2015) 58: 7672-7680 [PMID:26331334]
ChEMBL	Inhibition of recombinant full length C-terminal His/Flag-tagged human HDAC1 (1 to 482 residues) using Z-Lys(Ac)-AMC as substrate preincubated for 90 mins followed by trypsin addition and measured after 30 mins by fluorescence based microplate reader analysis	B	7.06	pIC50	88	nM	IC50	J Med Chem (2022) 65: 16860-16878 [PMID:36473103]
ChEMBL	Inhibition of HDAC1 (unknown origin) measured after 30 mins by fluorescence microplate reader assay	B	7.06	pIC50	87.9	nM	IC50	J Med Chem (2021) 64: 15280-15296 [PMID:34624191]
ChEMBL	Inhibition of HDAC1 (unknown origin) using (FAM)-labeled acetylated peptide as substrate measured after 17 hrs by fluorescence assay	B	7.06	pIC50	87.4	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of full length recombinant human C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected sf9 cells using Boc-Lys-(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	7.07	pIC50	85	nM	IC50	Bioorg Med Chem (2018) 26: 1418-1425 [PMID:29500130]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-pNA as substrate preincubated for 20 mins followed by substrate addition and further incubated for 90 mins by fluorescence assay	B	7.09	pIC50	82	nM	IC50	J Med Chem (2020) 63: 6959-6978 [PMID:32551649]
ChEMBL	Inhibition of full length recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 end residues) expressed in baculovirus infected Sf9 cells using fluorogenic HDAC substrate 3 measured after 30 mins by fluorescence based assay	B	7.09	pIC50	81	nM	IC50	J Med Chem (2018) 61: 1552-1575 [PMID:29360358]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using fluorometric substrate measured after 60 mins by FRET assay	B	7.1	pIC50	80	nM	IC50	J Med Chem (2021) 64: 4020-4033 [PMID:33745280]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic p53 (379-382 residues) (RHKK(Ac)AMC) as substrate	B	7.11	pIC50	77	nM	IC50	Eur J Med Chem (2023) 247: 115022-115022 [PMID:36549114]
ChEMBL	Inhibition of purified recombinant HDAC1	B	7.11	pIC50	77	nM	IC50	Bioorg Med Chem Lett (2011) 21: 4909-4912 [PMID:21742496]
ChEMBL	Inhibition of recombinant human HDAC1 using Cbz-Lys(TFAc)-AMC as substrate by fluorometric analysis	B	7.12	pIC50	76	nM	IC50	Bioorg Med Chem Lett (2010) 20: 7067-7070 [PMID:20947351]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.12	pIC50	76	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated with enzyme for 5 mins prior to substrate addition measured after 30 mins by fluorescence assay	B	7.12	pIC50	76	nM	IC50	ACS Med Chem Lett (2013) 4: 235-238 [PMID:23493449]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 5 mins followed by substrate addition and measured after 30 mins by multimode microplate reader analysis	B	7.12	pIC50	75	nM	IC50	J Med Chem (2022) 65: 12838-12859 [PMID:36153841]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.14	pIC50	73	nM	IC50	J Med Chem (2021) 64: 26-41 [PMID:33346659]
ChEMBL	Inhibition of HDAC1 (unknown origin) pre-incubated for 5 mins before fluorogenic substrate Boc-Lys (acetyl)-AMC or Boc-Lys (triflouroacetyl)-AMC addition and measured after 30 mins by fluorescence based assay	B	7.14	pIC50	71.8	nM	IC50	J Med Chem (2020) 63: 4701-4715 [PMID:32267687]
ChEMBL	Inhibition of C-terminal 6His/FLAG-tagged human recombinant full-length HDAC1 (1 to 482(end) residues) using Boc-Lys-(Ac)-AMC as substrate incubated for 10 mins followed by substrate addition measured after 1 hr by fluorescence based analysis	B	7.15	pIC50	70.65	nM	IC50	J Med Chem (2023) 66: 11761-11791 [PMID:37585683]
ChEMBL	Inhibition of HDAC1 in human HeLa cell nuclear extract using Boc-Lys(Ac)-AMC or Boc-Lys(triflouroacetyl)-AMC as substrate incubated for 30 mins and measured by fluorescence assay	B	7.15	pIC50	70	nM	IC50	Eur J Med Chem (2022) 241: 114634-114634 [PMID:35939996]
ChEMBL	Inhibition of full length recombinant C-terminal GST-tagged human HDAC1 expressed in baculovirus infected Sf9 insect cells incubated for 50 mins by Glo- luminescence assay	B	7.15	pIC50	70	nM	IC50	J Med Chem (2022) 65: 12346-12366 [PMID:36053318]
ChEMBL	Inhibition of human recombinant full-length HDAC1 (1 to 482 residues) expressed in baculovirus using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	7.16	pIC50	69	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.16	pIC50	69	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127128-127128 [PMID:32247729]
ChEMBL	Inhibition of recombinant HDAC1 using Ac-Lys(Ac)-AMC as substrate after 30 mins by fluorescence analysis	B	7.16	pIC50	69	nM	IC50	ACS Med Chem Lett (2012) 3: 505-508 [PMID:24900500]
ChEMBL	Inhibition of human recombinant HDAC1	B	7.17	pIC50	68.1	nM	IC50	Eur J Med Chem (2009) 44: 1067-1085 [PMID:18672316]
ChEMBL	Inhibition of HDAC1	B	7.17	pIC50	68	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of HDAC1 in human HeLa cells using BOC-K(Ac)-AMC as substrate measured after 30 mins by fluorescence based microtiter plate reader assay	B	7.18	pIC50	66	nM	IC50	J Med Chem (2022) 65: 11187-11213 [PMID:35926141]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.18	pIC50	65.4	nM	IC50	Eur J Med Chem (2015) 89: 628-637 [PMID:25462271]
ChEMBL	Inhibition of human HDAC1 using H3(1-21)K9 after 60 mins by fluorescence assay	B	7.19	pIC50	65.2	nM	IC50	J Nat Prod (2017) 80: 201-204 [PMID:28026946]
ChEMBL	Inhibition of human HDAC1 after 30 mins by microplate reader analysis	B	7.19	pIC50	65	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127023-127023 [PMID:32067866]
ChEMBL	Inhibition of full-length recombinant human HDAC1 (1 to 482 residues) expressed in baculovirus infected insect cells using Ac-Leu-Gly-Lys(Tfa)-AMC as substrate incubated for 30 mins by fluorescence based analysis	B	7.19	pIC50	64	nM	IC50	J Med Chem (2023) 66: 5950-5964 [PMID:37037787]
ChEMBL	Inhibition of C-terminal FLAG/His-tagged full length recombinant human HDAC1 expressed in baculovirus infected Sf9 insect cells using KI 177 as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by fluorescence assay	B	7.2	pIC50	63.5	nM	IC50	Eur J Med Chem (2018) 143: 792-805 [PMID:29223096]
ChEMBL	Inhibition of HDAC1 using KI-177 as substrate incubated 30 mins before substrate addition measured after 30 mins post substrate addition by fluorescence assay	B	7.2	pIC50	62.9	nM	IC50	Eur J Med Chem (2011) 46: 4042-4049 [PMID:21712146]
ChEMBL	Inhibition of human HDAC1 using ArgHisLysLys(Ac) fluorogenic peptide as a substrate by fluorimetric assay	B	7.21	pIC50	61	nM	IC50	J Med Chem (2011) 54: 2165-2182 [PMID:21417297]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) measured after 30 mins by fluorescence assay	B	7.21	pIC50	61	nM	IC50	Eur J Med Chem (2019) 180: 171-190 [PMID:31306905]
ChEMBL	Inhibition of HDAC1 (unknown origin) using FAM-RHKK(Ac)-NH2/FAM-RHKK(trifluoroacetyl)-NH2 as substrate preincubated for 15 mins followed by substrate addition and measured after 3 hrs by microfluidic chip based fluorescence assay	B	7.22	pIC50	60.7	nM	IC50	Bioorg Med Chem (2022) 56: 116599-116599 [PMID:35041998]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-AMC as substrate incubated for 30 mins by fluorescence based analysis	B	7.22	pIC50	60	nM	IC50	J Med Chem (2023) 66: 14150-14174 [PMID:37796543]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.23	pIC50	59	nM	IC50	Bioorg Med Chem Lett (2017) 27: 3289-3293 [PMID:28648462]
ChEMBL	Inhibition of human recombinant full length HDAC1 using fluorogenic substrate 3 after 30 mins by fluorescence assay	B	7.25	pIC50	56	nM	IC50	J Med Chem (2018) 61: 7245-7260 [PMID:30045621]
ChEMBL	Inhibition of full-length recombinant human C-terminal GST tagged HDAC1 expressed in Sf9 insect cells using aminoluciferin peptide substrate preincubated for 3 hrs followed by substrate addition and measured after 30 mins by HDAC-Glo I/II chemiluminescence assay	B	7.26	pIC50	55	nM	IC50	J Med Chem (2023) 66: 15171-15188 [PMID:37847303]
ChEMBL	Inhibition of HDAC1 (unknown origin) by fluorescence based assay	B	7.26	pIC50	54.6	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 120 mins	B	7.27	pIC50	53.78	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.28	pIC50	52	nM	IC50	Bioorg Med Chem (2023) 92: 117437-117437 [PMID:37563016]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.28	pIC50	52	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3346-3348 [PMID:23591111]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 90 mins	B	7.29	pIC50	51.75	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of recombinant full length human HDAC1 (1 to 482 amino acids) expressed in Baculovirus infected insect Sf9 cells incubated for 30 mins measured by fluorescence assay	B	7.29	pIC50	51	nM	IC50	Medchemcomm (2014) 5: 1887-1891
ChEMBL	Inhibition of HDAC1 in human HeLa nuclear extract	B	7.29	pIC50	51	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of human recombinant HDAC1	B	7.3	pIC50	50	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of HDAC1 in human HeLa nuclear extract using Boc-Lys(Ac)-AMC as substrate measured after 2 hrs by fluorescence based assay	B	7.3	pIC50	50	nM	IC50	J Med Chem (2018) 61: 1552-1575 [PMID:29360358]
ChEMBL	Inhibition of human recombinant HDAC1 using Ac-Leu-Gly-Lys(Ac)-AMC as substrate by measuring fluorescence intensity incubated for 30 mins by microplate reader assay	B	7.31	pIC50	49	nM	IC50	Eur J Med Chem (2021) 217: 113363-113363 [PMID:33744687]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2020) 185: 111725-111725 [PMID:31655430]
ChEMBL	Inhibition of HDAC1	B	7.32	pIC50	48	nM	IC50	J Med Chem (2008) 51: 7053-7056 [PMID:18942826]
ChEMBL	Inhibition of human HDAC 1	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem Lett (2007) 17: 3969-3971 [PMID:17507219]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 60 mins	B	7.32	pIC50	47.95	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of human recombinant HDAC1 after 15 mins by fluorogenic assay	B	7.32	pIC50	47.4	nM	IC50	J Med Chem (2015) 58: 4550-4572 [PMID:25993269]
ChEMBL	Inhibition of full length recombinant FLAG/His-tagged C-terminal human HDAC1 expressed in baculovirus infected Sf9 insect cells using ZMAL (Z-Lys(Ac)-AMC) as substrate incubated for 90 mins by fluorescence based assay	B	7.33	pIC50	47	nM	IC50	ACS Med Chem Lett (2019) 10: 1336-1340 [PMID:31531206]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	7.34	pIC50	46	nM	IC50	ACS Med Chem Lett (2019) 10: 1122-1127 [PMID:31413795]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition and further incubated for incubated for 1 hrs by fluorescence microtiter plate reader assay	B	7.34	pIC50	45.5	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of C-terminal FLAG/His-tagged recombinant human HDAC1 expressed in baculovirus infected Sf9 insect cells using Z-(Ac)Lys-AMC as substrate measured after 90 mins by fluorescence assay	B	7.35	pIC50	45	nM	IC50	J Med Chem (2017) 60: 5493-5506 [PMID:28574690]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.35	pIC50	45	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4885-4888 [PMID:28947154]
ChEMBL	Inhibition of recombinant full length human C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected sf9 cells using Z-(Ac)Lys-AMC as substrate measured after 90 mins by fluorescence assay	B	7.35	pIC50	45	nM	IC50	J Med Chem (2017) 60: 5334-5348 [PMID:28581289]
ChEMBL	Inhibition of recombinant human full length HDAC1 expressed in baculovirus infected Sf9 insect cells using biotinylated lysine 9 acetylated histone H3 (1 to 21 residues) as substrate incubated for 5 mins followed by substrate addition measured after 60 mins by HTRF assay	B	7.36	pIC50	44	nM	IC50	ACS Med Chem Lett (2017) 8: 830-834 [PMID:28835797]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic HDAC substrate incubated for 30 mins by fluorescence assay	B	7.36	pIC50	44	nM	IC50	Eur J Med Chem (2020) 187: 111970-111970 [PMID:31881454]
ChEMBL	Inhibition of human HDAC1 incubated for 30 mins by SpectraMax M2 microplate reader analysis	B	7.36	pIC50	44	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of human HDAC1 expressed in Escherichia coli using fluorogenic substrate treated for 5 mins before substrate addition measured after 30 mins by fluorescence assay	B	7.36	pIC50	44	nM	IC50	Eur J Med Chem (2011) 46: 5387-5397 [PMID:21924799]
ChEMBL	Inhibition of full length human C-terminal His-tagged/C-terminal Flag-tagged HDAC1 using fluorogenic acetylated peptide as substrate incubated for 30 mins by fluorescence plate reader assay	B	7.36	pIC50	43.9	nM	IC50	J Med Chem (2021) 64: 3392-3426 [PMID:33661013]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.36	pIC50	43.76	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based micro plate reader analysis	B	7.36	pIC50	43.76	nM	IC50	J Med Chem (2020) 63: 5501-5525 [PMID:32321249]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.37	pIC50	43	nM	IC50	Eur J Med Chem (2016) 108: 274-286 [PMID:26689485]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 30 mins	B	7.37	pIC50	42.82	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.37	pIC50	42.5	nM	IC50	J Med Chem (2019) 62: 3171-3183 [PMID:30418766]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	7.38	pIC50	42	nM	IC50	Bioorg Med Chem Lett (2019) 29: 2638-2645 [PMID:31400938]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.38	pIC50	42	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic HDAC substrate preincubated for 30 mins followed by substrate addition and measured after 15 mins by fluorogenic assay	B	7.38	pIC50	42	nM	IC50	Bioorg Med Chem (2020) 28: 115345-115345 [PMID:32061484]
ChEMBL	Inhibition of human recombinant HDAC1 after 30 mins by fluorescence assay	B	7.38	pIC50	42	nM	IC50	J Med Chem (2013) 56: 5782-5796 [PMID:23786452]
ChEMBL	Inhibition of human full-length recombinant HDAC1 preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of HDAC1 in human HeLa cells nuclear extract using Fluor-de-Lys as substrate after 30 mins by spectrophotometry	B	7.39	pIC50	41	nM	IC50	ACS Med Chem Lett (2011) 2: 703-707 [PMID:21984958]
ChEMBL	Inhibition of recombinant human HDAC1 using Fluor-de-Lys as substrate after 30 mins by spectrophotometry	B	7.39	pIC50	41	nM	IC50	ACS Med Chem Lett (2011) 2: 703-707 [PMID:21984958]
ChEMBL	Inhibition of full length human recombinant HDAC1 expressed in baculovirus expression system using (Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	7.4	pIC50	40	nM	IC50	J Med Chem (2018) 61: 6056-6074 [PMID:29940115]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.4	pIC50	40	nM	IC50	Eur J Med Chem (2021) 219: 113419-113419 [PMID:33845233]
ChEMBL	Inhibition of full-length recombinant human HDAC1 (1 to 482 residues) expressed in baculovirus infected insect cells using Ac-Leu-Gly-Lys(Ac)-AMC as substrate incubated for 30 mins by fluorescence based assay	B	7.4	pIC50	40	nM	IC50	J Med Chem (2023) 66: 1239-1253 [PMID:36622852]
ChEMBL	Inhibition of recombinant human HDAC1 using Boc-Lys-(acetyl)-AMC as substrate incubated for 0.5 hr and measured after 20 mins by fluorescence assay	B	7.4	pIC50	40	nM	IC50	Eur J Med Chem (2022) 233: 114228-114228 [PMID:35245830]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 5 mins	B	7.4	pIC50	39.91	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HDAC1 (unknown origin) in using Boc-Lys(acetyl)-AMC or Boc-Lys (triflouroacetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	7.4	pIC50	39.8	nM	IC50	J Med Chem (2020) 63: 12083-12099 [PMID:33021789]
ChEMBL	Inhibition of His-tagged HDAC1 (unknown origin) expressed in Escherichia coli BL21(DE3) after 60 mins by MALDI mass spectrometry	B	7.42	pIC50	38	nM	IC50	Eur J Med Chem (2015) 96: 340-359 [PMID:25899338]
ChEMBL	Inhibition of human HDAC1 preincubated for 15 mins followed by substrate addition by fluorescence-based assay	B	7.42	pIC50	38	nM	IC50	Eur J Med Chem (2021) 218: 113383-113383 [PMID:33799069]
ChEMBL	Inhibition of HDAC1 in human HeLa cell nuclear extract using Fluor de Lys as substrate after 15 mins by fluorometric analysis	B	7.42	pIC50	38	nM	IC50	J Med Chem (2012) 55: 1465-1477 [PMID:22260166]
ChEMBL	Inhibition of HDAC1 (unknown origin) at 10 uM after 60 mins by SAMDI spectrophotometric analysis relative to control	B	7.42	pIC50	38	nM	IC50	J Med Chem (2013) 56: 3492-3506 [PMID:23547652]
ChEMBL	Inhibition of HDAC1 (unknown origin) expressed in Escherichia coli BL21 (DE3) using GRKacFGC as substrate after 60 mins by SAMDI mass spectrometric analysis	B	7.42	pIC50	38	nM	IC50	J Med Chem (2013) 56: 9969-9981 [PMID:24304348]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using Boc-Lys (Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	7.42	pIC50	38	nM	IC50	Eur J Med Chem (2020) 188: 111991-111991 [PMID:31883490]
ChEMBL	Inhibition of full length human recombinant HDAC1 expressed in baculovirus using Ac-Leu-GlyLys(Ac)-AMC as substrate after 30 mins by fluorescence assay	B	7.42	pIC50	38	nM	IC50	J Med Chem (2015) 58: 4325-4338 [PMID:25906087]
ChEMBL	Inhibition of HDAC1 in human HeLa cells using Fluor-de-Lys as substrate after 15 mins by fluorescence assay	B	7.42	pIC50	38	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3283-3287 [PMID:23622981]
ChEMBL	Inhibition of recombinant human HDAC1 using Boc-Lys(Ac)-AMC as substrate incubated for 60 mins by fluorimetric assay	B	7.42	pIC50	37.7	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127638-127638 [PMID:33132117]
ChEMBL	Inhibition of full length recombinant human C-terminal FLAG-tagged HDAC1 expressed in sf9 cells preincubated for 5 mins followed by biotinylated H3K9-Ac substrate addition and measured after 60 mins by HTRF assay	B	7.46	pIC50	35	nM	IC50	Bioorg Med Chem Lett (2019) 29: 349-352 [PMID:30594434]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	7.46	pIC50	34.6	nM	IC50	J Med Chem (2014) 57: 3324-3341 [PMID:24694055]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-L-Lys (Ac)-AMC as substrate preincubated with compound for 3 hrs measured after 35 mins by spectrofluorometric analysis	B	7.47	pIC50	34	nM	IC50	Bioorg Med Chem Lett (2012) 22: 5025-5030 [PMID:22771007]
ChEMBL	Inhibition of human recombinant HDAC1 using MAZ1600 as fluorogenic substrate measured every 5 mins by optimized homogenous fluorescence based assay	B	7.47	pIC50	34	nM	IC50	J Med Chem (2015) 58: 4812-4821 [PMID:25974739]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in Escherichia coli BL21(DE3) cells using KL177 as substrate preincubated for 5 followed by substrate addition and measured after 30 mins by fluorescence based assay	B	7.47	pIC50	33.5	nM	IC50	ACS Med Chem Lett (2020) 11: 713-719 [PMID:32435375]
ChEMBL	Inhibition of recombinant HDAC1 expressed in HEK293 cells	B	7.48	pIC50	33	nM	IC50	J Med Chem (2008) 51: 3985-4001 [PMID:18558669]
ChEMBL	Inhibition of HDAC1 (unknown origin) by ELISA-based assay	B	7.48	pIC50	33	nM	IC50	Bioorg Med Chem Lett (2017) 27: 3254-3258 [PMID:28648461]
ChEMBL	Inhibition of HADC1 (unknown origin)	B	7.48	pIC50	33	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of human recombinant HDAC1 using ZMAL (Z-(Ac)Lys-AMC) as fluorogenic substrate incubated for 120 mins by microplate reader assay	B	7.48	pIC50	33	nM	IC50	J Med Chem (2021) 64: 9960-9988 [PMID:34251197]
ChEMBL	Inhibition of HDAC1 in human HeLaS3 cells preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 45 mins by ELISA	B	7.48	pIC50	33	nM	IC50	ACS Med Chem Lett (2017) 8: 281-286 [PMID:28337317]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.48	pIC50	33	nM	IC50	J Med Chem (2022) 65: 3080-3097 [PMID:35148101]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.48	pIC50	33	nM	IC50	Bioorg Med Chem Lett (2021) 47: 128207-128207 [PMID:34146703]
ChEMBL	Inhibition of HDAC1 in human HeLa-S3 cell lysates preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 40 mins by fluorimetric method	B	7.48	pIC50	33	nM	IC50	Eur J Med Chem (2018) 143: 1790-1806 [PMID:29150330]
ChEMBL	Inhibition of full length his6/FLAG-tagged human recombinant HDAC1(1 to 482 residues) expressed in Sf9 insect cells	B	7.48	pIC50	33	nM	IC50	J Med Chem (2022) 65: 14764-14791 [PMID:36306372]
ChEMBL	Inhibition of recombinant human His-tagged HDAC1 expressed in insect cells using Ac-Leu-Gly-Lys(Ac)-AMC as substrate measured after 30 mins by fluorescence assay	B	7.49	pIC50	32.33	nM	IC50	Eur J Med Chem (2020) 205: 112648-112648 [PMID:32791401]
ChEMBL	Inhibition of recombinant C-terminal His/FLAG-tagged HDAC1 (unknown origin) expressed in baculovirus infected Sf9 insect cells using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr	B	7.49	pIC50	32	nM	IC50	J Med Chem (2019) 62: 6992-7014 [PMID:31117517]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.51	pIC50	31	nM	IC50	Bioorg Med Chem Lett (2017) 27: 744-749 [PMID:28131715]
ChEMBL	Inhibition of recombinant human HDAC1 using Ac-GAK(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence-based assay	B	7.51	pIC50	31	nM	IC50	J Med Chem (2021) 64: 4810-4840 [PMID:33830764]
ChEMBL	Inhibition of HDAC1 in human HeLa cells after 72 hrs by ELISA	B	7.51	pIC50	31	nM	IC50	Eur J Med Chem (2018) 146: 79-92 [PMID:29396364]
ChEMBL	Inhibition of recombinant human full length HDAC1 expressed in baculovirus infected Sf9 insect cells using p53 (379 to 382 residues) derived RHKKAc as substrate preincubated for 5 to 10 mins followed by substrate addition measured after 2 hrs by fluorescence assay	B	7.51	pIC50	31	nM	IC50	ACS Med Chem Lett (2017) 8: 1031-1036 [PMID:29057046]
ChEMBL	Inhibition of HDAC1 in HEK293 cells	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem Lett (2008) 18: 1814-1819 [PMID:18308563]
ChEMBL	Inhibition of human HDAC1 pre-incubated for 30 mins before substrate addition and measured after 30 mins by HDAC-Glo I/II assay	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem (2014) 22: 3720-3731 [PMID:24864038]
ChEMBL	Inhibition of human recombinant full length HDAC1 using (Ac)Arg-Gly-Lys(Ac) as substrate after 60 mins by fluorescence assay	B	7.52	pIC50	30	nM	IC50	J Med Chem (2013) 56: 9934-9954 [PMID:24261862]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-lys(Ac)-AMC as substrate preincubated for 20 mins with substrate measured after 60 mins by fluorescence analysis	B	7.52	pIC50	30	nM	IC50	J Med Chem (2012) 55: 1731-1750 [PMID:22280363]
ChEMBL	Inhibition of human recombinant HDAC1 using fluor de Lys as substrate by fluorometric analysis	B	7.52	pIC50	30	nM	IC50	J Med Chem (2010) 53: 8663-8678 [PMID:21080647]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.52	pIC50	30	nM	IC50	J Med Chem (2021) 64: 3392-3426 [PMID:33661013]
ChEMBL	Inhibition of human C-terminal FLAG-tagged HDAC1 in HEK293 cells	B	7.52	pIC50	30	nM	IC50	J Med Chem (2009) 52: 3453-3456 [PMID:19441846]
ChEMBL	Inhibition of HDAC1 (unknown origin) by colorimetric method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of human recombinant C-terminally flag tagged HDAC1	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5528-5532 [PMID:18809328]
ChEMBL	Inhibition of HDAC1	B	7.52	pIC50	30	nM	IC50	J Med Chem (2008) 51: 2350-2353 [PMID:18370373]
ChEMBL	Inhibition of HDAC1	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem Lett (2008) 18: 726-731 [PMID:18060775]
ChEMBL	Inhibition of recombinant human HDAC1 expressed in baculovirus expression system using Ac-Leu-Gly-Lys (Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence microtiter plate reader assay	B	7.54	pIC50	29	nM	IC50	J Med Chem (2022) 65: 2434-2457 [PMID:35043615]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-L-Lys (Ac)-AMC as substrate preincubated with compound for 5 mins measured after 35 mins by spectrofluorometric analysis	B	7.54	pIC50	29	nM	IC50	Bioorg Med Chem Lett (2012) 22: 5025-5030 [PMID:22771007]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.54	pIC50	29	nM	IC50	Bioorg Med Chem Lett (2023) 94: 129462-129462 [PMID:37652098]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.57	pIC50	27	nM	IC50	J Med Chem (2018) 61: 3166-3192 [PMID:29518312]
ChEMBL	Inhibition of HDAC1 (mean IC50)	B	7.57	pIC50	27	nM	IC50	Bioorg Med Chem Lett (2006) 16: 5948-5952 [PMID:16987657]
ChEMBL	Inhibition of HDAC1 by fluorogenic enzyme assay	B	7.57	pIC50	27	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3651-3656 [PMID:19457659]
ChEMBL	Inhibition of human recombinant HDAC1 (1 to 482 residues) expressed in Baculovirus using Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by fluorescence assay	B	7.59	pIC50	26	nM	IC50	J Med Chem (2017) 60: 7965-7983 [PMID:28885834]
ChEMBL	Inhibition of recombinant full length human C-terminal His/flag-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells after 30 mins by fluorescence assay	B	7.59	pIC50	26	nM	IC50	ACS Med Chem Lett (2018) 9: 34-38 [PMID:29348808]
ChEMBL	Inhibition of HDAC1 (unknown origin) incubated for 30 mins by microplate reader assay	B	7.59	pIC50	25.8	nM	IC50	Eur J Med Chem (2022) 229: 114058-114058 [PMID:34954595]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) expressed in baculovirus expression system using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition by fluorescence based protease coupled assay	B	7.6	pIC50	25	nM	IC50	Medchemcomm (2019) 10: 1828-1837 [PMID:32180916]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using fluor de lys SIRT1 as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	7.6	pIC50	25	nM	IC50	Eur J Med Chem (2020) 192: 112193-112193 [PMID:32151835]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorimetric substrate incubated for 30 mins by fluorogenic assay	B	7.6	pIC50	25	nM	IC50	Eur J Med Chem (2022) 227: 113946-113946 [PMID:34775332]
ChEMBL	Inhibition of full-length recombinant human HDAC1 expressed in Baculovirus expression system using Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and further incubated for 30 mins by fluorescence based microtiter plate reader assay	B	7.62	pIC50	24	nM	IC50	J Med Chem (2022) 65: 4818-4831 [PMID:35238576]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.64	pIC50	23.04	nM	IC50	Bioorg Med Chem Lett (2023) 88: 129305-129305 [PMID:37116762]
ChEMBL	Inhibition of purified human HDAC1 using Ac-Leu-Gly-(e-Ac)Lys-AMC by fluorometric HDAC assay	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem Lett (2022) 55: 128441-128441 [PMID:34767912]
ChEMBL	Inhibition of recombinant full length human HDAC1 (1 to 482) expressed in baculovirus infected insect Sf9 cells using fluorogenic HDAC substrate 3 after 30 mins by fluorescence assay	B	7.64	pIC50	23	nM	IC50	ACS Med Chem Lett (2015) 6: 239-243 [PMID:25815139]
ChEMBL	Inhibition of human HDAC1 pre-incubated for 5 mins before substrate addition and measured after 30 mins by fluorescence based assay	B	7.64	pIC50	22.7	nM	IC50	J Med Chem (2021) 64: 1116-1126 [PMID:33356256]
ChEMBL	Inhibition of recombinant human FLAG/His-tagged HDAC1 expressed in baculovirus expression system using Boc-l-Lys(Ac)-AMC as substrate preincubated up to 3 hrs and measured after 35 mins by fluorescence assay	B	7.65	pIC50	22.4	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.65	pIC50	22.4	nM	IC50	Medchemcomm (2014) 5: 1829-1833
ChEMBL	Inhibition of human recombinant HDAC1 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	7.65	pIC50	22.37	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-Lys(Ac)-AMC as substrate incubated for 1 hrs in presence of illumination with 550 nm light by fluorescence based assay	B	7.66	pIC50	22	nM	IC50	J Med Chem (2023) 66: 1909-1927 [PMID:36654474]
ChEMBL	Inhibition of HDAC1	B	7.68	pIC50	21	nM	IC50	J Med Chem (2008) 51: 1505-1529 [PMID:18247554]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in HEK293 cells	B	7.68	pIC50	21	nM	IC50	J Med Chem (2010) 53: 8546-8555 [PMID:21080629]
ChEMBL	Inhibition of human recombinant HDAC1 using Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 30 mins followed by substrate addition and measured after 15 to 30 mins	B	7.68	pIC50	21	nM	IC50	J Med Chem (2018) 61: 3779-3798 [PMID:29039662]
ChEMBL	Inhibition of HDAC1 (unknown origin) using trypsin and LGK(Ac)-AMC as substrates incubated for 1 hr and measured by fluorescence assay	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2022) 236: 114326-114326 [PMID:35390714]
ChEMBL	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	B	7.68	pIC50	20.9	nM	IC50	US-9115116-B2. Dual action inhibitors against histone deacetylases and 3-hydroxy-3-methylglutaryl coenzyme a reductase (2015)
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) after 10 mins by fluorimetric analysis	B	7.68	pIC50	20.9	nM	IC50	J Med Chem (2013) 56: 3645-3655 [PMID:23570542]
ChEMBL	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	B	7.68	pIC50	20.9	nM	IC50	US-9353061-B2. 3,5,N-trihydroxy-alkanamide and derivatives: method for making same and use thereof (2016)
ChEMBL	Inhibition of human recombinant HDAC1 expressed in HEK293 cells	B	7.7	pIC50	20	nM	IC50	J Med Chem (2009) 52: 2265-2279 [PMID:19301902]
ChEMBL	Inhibition of recombinant full length HDAC1 (unknown origin) using Ac-peptide-AMC as substrate after 1 hr by fluorescence assay	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2019) 178: 116-130 [PMID:31177073]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-peptide as a substrate pretreated for 15 mins followed by substrate addition	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2021) 225: 113824-113824 [PMID:34509167]
ChEMBL	Inhibition of recombinant HDAC1 in HeLa cells	B	7.7	pIC50	20	nM	IC50	J Med Chem (2007) 50: 4405-4418 [PMID:17691763]
ChEMBL	Inhibition of recombinant full length C-terminal FLAG/His-tagged human HDAC1 expressed in baculovirus infected Sf9 cells using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2019) 182: 111672-111672 [PMID:31505452]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.72	pIC50	19	nM	IC50	J Med Chem (2023) 66: 4802-4826 [PMID:36934335]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.73	pIC50	18.55	nM	IC50	Eur J Med Chem (2020) 205: 112679-112679 [PMID:32791404]
ChEMBL	Inhibition of recombinant human HDAC1 using Ac-LGK(Ac)-AMC as substrate preincubated for 30 mins followed by substrate addition and measured after 90 mins by fluorescence based assay	B	7.74	pIC50	18	nM	IC50	J Med Chem (2021) 64: 2186-2204 [PMID:33570940]
ChEMBL	Inhibition of HDAC1 (unknown origin) using trypsin and Ac-peptide as substrates	B	7.76	pIC50	17.39	nM	IC50	Eur J Med Chem (2022) 230: 114115-114115 [PMID:35033824]
ChEMBL	Inhibition of HDAC1 (unknown origin) assessed as release of fluorogenic AMC preincubated for 15 mins followed by addition of trypsin and substrate measured after 60 mins Spectra max microplate reader analysis	B	7.77	pIC50	17	nM	IC50	J Med Chem (2023) 66: 4802-4826 [PMID:36934335]
ChEMBL	Inhibition of HDAC1 (unknown origin) using biotinylated histone H3 KAc peptide (1 to 21 residues) as substrate by HTRF assay	B	7.77	pIC50	17	nM	IC50	J Med Chem (2020) 63: 3678-3700 [PMID:32153186]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.77	pIC50	17	nM	IC50	J Med Chem (2022) 65: 16541-16569 [PMID:36449947]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.8	pIC50	16	nM	IC50	Eur J Med Chem (2021) 213: 113173-113173 [PMID:33493830]
ChEMBL	Inhibition of C-terminal His-tagged and C-terminal FLAG-tagged human HDAC1 expressed in baculovirus expression system using Ac-peptide substrate incubated for 15 mins by fluorescence based assay	B	7.8	pIC50	16	nM	IC50	Eur J Med Chem (2017) 140: 392-402 [PMID:28987602]
ChEMBL	Inhibition of recombinant human HDAC1 expressed in baculovirus infection system using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence assay	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem (2022) 53: 116524-116524 [PMID:34847495]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys (Ac)-AMC as substrate measured after 60 mins by ELISA	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2019) 29: 15-21 [PMID:30455152]
ChEMBL	Inhibition of HDAC1 (unknown origin) assessed as release of 7-amino-4-methylcoumarin incubated in room temperature for 15 min measured by Spectra max microtitre plate reader	B	7.84	pIC50	14.6	nM	IC50	J Med Chem (2021) 64: 16573-16597 [PMID:34783558]
ChEMBL	Inhibition of recombinant human full length HDAC1 using fluorogenic substrate 3 after 30 mins by fluorescence assay	B	7.84	pIC50	14.4	nM	IC50	ACS Med Chem Lett (2018) 9: 312-317 [PMID:29670692]
ChEMBL	Inhibition of C-terminal FLAG tagged recombinant human HDAC1 expressed in Sf9 cells using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition by fluorescence based analysis	B	7.84	pIC50	14.38	nM	IC50	Eur J Med Chem (2023) 254: 115367-115367 [PMID:37086699]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-peptide as substrate pretreated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	7.84	pIC50	14.3	nM	IC50	Bioorg Med Chem (2018) 26: 3958-3966 [PMID:29954683]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.85	pIC50	14.15	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127610-127610 [PMID:33045329]
ChEMBL	Inhibition of human C-terminal Flag tagged HDAC1 (1 to 482 residues) expressed in SF9 cells using Ac-peptide substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by microplate reader method	B	7.85	pIC50	14.15	nM	IC50	Eur J Med Chem (2020) 204: 112651-112651 [PMID:32738414]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition by fluorescence assay	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2021) 220: 113453-113453 [PMID:33957387]
ChEMBL	Inhibition of human recombinant HDAC1 by microplate reader assay	B	7.85	pIC50	14	nM	IC50	Medchemcomm (2015) 6: 613-618 [PMID:26005563]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-Lys(Ac)-AMC as substrate incubated for 1 hrs in presence of illumination with 420 nm light by fluorescence based assay	B	7.85	pIC50	14	nM	IC50	J Med Chem (2023) 66: 1909-1927 [PMID:36654474]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic Boc-Lys (aetyl)-AMC as substrate preincubated for 10 mins followed by substrate addition measured after 2 hrs by measuring fluorescence intensity using multimode microplate reader	B	7.88	pIC50	13.2	nM	IC50	J Med Chem (2023) 66: 10791-10807 [PMID:37498552]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-AMC as substrate by fluorescence assay	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem Lett (2014) 24: 4768-4772 [PMID:25182565]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem Lett (2022) 64: 128663-128663 [PMID:35272009]
ChEMBL	Inhibition of recombinant human C-terminal His/FLAG-tagged HDAC1 (1 to end residues) expressed in sf9 insect cells using Boc-Lys(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2020) 192: 112189-112189 [PMID:32151834]
ChEMBL	Inhibition of HDAC1 (unknown origin) measured by fluorescence based assay	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-Lys(Ac)-AMC as substrate incubated for 1 hrs in presence of illumination with 380 nm light by fluorescence based assay	B	7.89	pIC50	13	nM	IC50	J Med Chem (2023) 66: 1909-1927 [PMID:36654474]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in human HEK293 cells using Ac-NH-GGK(Ac)-AMC as substrate after 180 mins by fluorescence assay	B	7.89	pIC50	13	nM	IC50	Medchemcomm (2012) 3: 829-835
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of HDAC-1 (unknown origin) expressed in Escherichia coli BL21 (DE3) in HeLa nuclear extract using KI177 as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by Bradford reagent method	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of recombinant human C-terminal His/FLAG-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using Ac-peptide-AMC as substrate preincubated for 15 mins followed by addition of substrate and trypsin measured by flourescence assay	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2021) 224: 113672-113672 [PMID:34237620]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.92	pIC50	12	nM	IC50	Bioorg Med Chem Lett (2022) 72: 128846-128846 [PMID:35697181]
ChEMBL	Inhibition of HDAC1 (unknown origin) using acetylated peptide substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence analysis	B	7.95	pIC50	11.34	nM	IC50	Bioorg Med Chem (2017) 25: 4100-4109 [PMID:28601509]
ChEMBL	Inhibition of HDAC1 (unknown origin) preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	7.95	pIC50	11.11	nM	IC50	Eur J Med Chem (2017) 134: 281-292 [PMID:28419930]
ChEMBL	Inhibition of recombinant human full length C-terminal His/FLAG tagged HDAC1 (1 to 482 residues) expressed in baculovirus-infected Sf9 cells using Ac-peptide as substrate incubated for 4 hrs	B	7.96	pIC50	11	nM	IC50	J Med Chem (2022) 65: 4156-4181 [PMID:35175762]
ChEMBL	Inhibition of HDAC1	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
ChEMBL	Inhibition of full length C-terminal His/FLAG-tagged human recombinant HDAC1 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2016) 59: 5488-5504 [PMID:27186676]
ChEMBL	Inhibition of recombinant full length HDAC1 (unknown origin) using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2019) 62: 1577-1592 [PMID:30629434]
ChEMBL	Inhibition of human recombinant HDAC1 using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	7.96	pIC50	11	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem Lett (2023) 94: 129466-129466 [PMID:37660833]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-Lys(Ac)-AMC as substrate incubated for 1 hrs in presence of illumination with 365 nm light by fluorescence based assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2023) 66: 1909-1927 [PMID:36654474]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem Lett (2021) 49: 128286-128286 [PMID:34314844]
ChEMBL	Inhibition of HDAC1 (unknown origin) using acetylated peptide substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence analysis	B	7.97	pIC50	10.8	nM	IC50	Bioorg Med Chem (2017) 25: 27-37 [PMID:27769671]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-AMC as substrate incubated for 60 mins by fluorescence plate reader assay	B	7.97	pIC50	10.68	nM	IC50	Eur J Med Chem (2022) 227: 113893-113893 [PMID:34656899]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2019) 183: 111691-111691 [PMID:31536895]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence assay	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of recombinant human HDAC1 after 30 mins by fluorescence assay	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4051-4055 [PMID:28765013]
ChEMBL	Inhibition of recombinant human GST-fused HDAC1 expressed in HEK293 cells Fluor-de-lys as substrate measured after 60 mins by fluorescence assay	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of C-terminal FLAG/His-tagged human recombinant HDAC1 (1 to 482 residues) expressed in baculovirus-infected Sf9 cells assessed as reduction in 7-amino-4-methylcoumarin release using fluorogenic HDAC substrate 3 measured every 5 mins by fluorescence based analysis	B	8	pIC50	10	nM	IC50	Bioorg Med Chem (2021) 42: 116251-116251 [PMID:34116381]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2023) 94: 129466-129466 [PMID:37660833]
ChEMBL	Inhibition of HDAC1 in human Jurkat cells extract after 30 mins by immunoprecipitation assay	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2016) 26: 3122-3126 [PMID:27177826]
ChEMBL	Inhibition of human recombinant full-length HDAC1 using fluorophore conjugated substrate by fluorescence assay	B	8.01	pIC50	9.7	nM	IC50	J Med Chem (2011) 54: 5576-5582 [PMID:21721525]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Ac-Leu-GlyLys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by fluorescence analysis	B	8.05	pIC50	9	nM	IC50	Bioorg Med Chem (2017) 25: 4123-4132 [PMID:28629630]
ChEMBL	Inhibition of human recombinant HDAC1 after 30 mins by fluorometric assay	B	8.05	pIC50	8.9	nM	IC50	J Med Chem (2012) 55: 3777-3791 [PMID:22439863]
ChEMBL	Inhibition of human recombinant HDAC1 using acetyllysine tripeptide coupled with 7-amino-4-methylcoumarin as substrate by fluorescence assay	B	8.15	pIC50	7	nM	IC50	Bioorg Med Chem Lett (2016) 26: 1265-1271 [PMID:26804233]
ChEMBL	Inhibition of human KDAC1	B	8.15	pIC50	7	nM	IC50	J Med Chem (2016) 59: 1613-1633 [PMID:26681404]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic substrate incubated for 30 mins by fluorescence based assay	B	8.28	pIC50	5.2	nM	IC50	J Med Chem (2022) 65: 3667-3683 [PMID:35152694]
ChEMBL	Inhibition of HDAC1 (unknown origin) after 60 mins by fluorescence assay	B	8.3	pIC50	5	nM	IC50	J Med Chem (2013) 56: 4816-4820 [PMID:23672185]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Fluor de lys as substrate by fluorescence based analysis	B	8.37	pIC50	4.24	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic peptide RHKKAc-AMC as substrate by fluorescence-based assay	B	8.37	pIC50	4.24	nM	IC50	Bioorg Med Chem Lett (2023) 81: 129148-129148 [PMID:36690041]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Fluor de Lys substrate by fluorescence assay relative to control	B	8.4	pIC50	4	nM	IC50	Eur J Med Chem (2019) 168: 110-122 [PMID:30802729]
ChEMBL	Inhibition of HDAC1 (unknown origin) assessed as fluorescence intensity measured after 60 mins incubation at room temperature by trypsin-free microfluidic lab-on-a-chip assay	B	8.4	pIC50	4	nM	IC50	J Med Chem (2013) 56: 1772-1776 [PMID:23368884]
ChEMBL	Inhibition of human recombinant HDAC1 using Boc-Lys(Ac)-AMC as substrate incubated for 1 hrs in dark by fluorescence based assay	B	8.52	pIC50	3	nM	IC50	J Med Chem (2023) 66: 1909-1927 [PMID:36654474]
ChEMBL	Inhibition of recombinant human HDAC1	B	8.7	pIC50	2	nM	IC50	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of HDAC1 in human HeLa cells nuclear extract incubated for 30 mins by multiplate reader analysis	B	9.07	pIC50	0.85	nM	IC50	J Med Chem (2023) 66: 5753-5773 [PMID:37057760]
histone deacetylase 1/Histone deacetylase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4001] [GtoPdb: 2658] [UniProtKB: O09106]
ChEMBL	Binding affinity towards HDAC1 (Histone deacetylase 1) in mouse A20 cells, expressed as binding constant (pKi)	B	7.17	pKi	67.61	nM	Ki	J Med Chem (2002) 45: 1778-1784 [PMID:11960489]
ChEMBL	Inhibition of mouse HDAC1	B	6.95	pIC50	112.2	nM	IC50	J Med Chem (2004) 47: 1351-1359 [PMID:14998325]
ChEMBL	In vitro for anti-HDAC1 (Histone deacetylase 1) activity in mouse A20 cells	B	6.95	pIC50	112	nM	IC50	J Med Chem (2002) 45: 1778-1784 [PMID:11960489]
ChEMBL	Inhibition of mouse Histone deacetylase 1 (HDAC1) receptor	B	6.95	pIC50	112	nM	IC50	J Med Chem (2004) 47: 1351-1359 [PMID:14998325]
ChEMBL	Inhibition of mouse HDAC1	B	6.95	pIC50	112	nM	IC50	J Med Chem (2006) 49: 6046-6056 [PMID:17004718]
ChEMBL	Inhibition of mouse liver HDAC1	B	6.95	pIC50	112	nM	IC50	J Med Chem (2008) 51: 1505-1529 [PMID:18247554]
histone deacetylase 10/Histone deacetylase 10 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8]
ChEMBL	Competitive inhibition of HDAC10 using KI-104 as substrate by fluorescence assay	B	7.22	pKi	60	nM	Ki	J Med Chem (2011) 54: 4694-4720 [PMID:21634430]
GtoPdb	-	-	7.3	pKi	50	nM	Ki	Bioorg Med Chem Lett (2010) 20: 3314-21 [PMID:20451378]
ChEMBL	Inhibition of HDAC10	B	7.3	pKi	50	nM	Ki	Bioorg Med Chem Lett (2010) 20: 3314-3321 [PMID:20451378]
ChEMBL	Inhibition of human recombinant HDAC10 using Ac-Arg-His-Lys(Ac)-Lys(Ac)-AMC substrate incubated for 15 to 30 mins	B	7.4	pKi	40	nM	Ki	J Med Chem (2014) 57: 9644-9657 [PMID:25380299]
ChEMBL	Inhibition of human recombinant HDAC10 using Ac-Arg-His-Lys (Ac)-Lys (Ac)-AMC substrate incubated for 30 mins by fluorescence assay	B	7.4	pKi	40	nM	Ki	Medchemcomm (2014) 5: 1849-1855
ChEMBL	Inhibition of dye-labeled tracer binding to HDAC10 (unknown origin) transfected in human HeLa cells measured after 2 hrs by nano-luciferase reporter gene-based BRET assay	B	5.9	pIC50	1258.93	nM	IC50	J Med Chem (2019) 62: 4426-4443 [PMID:30964290]
ChEMBL	Inhibition of HDAC 10 in human HeLa nuclear extract measured after 60 mins by fluorometric analysis	B	6.01	pIC50	982	nM	IC50	J Med Chem (2017) 60: 3484-3497 [PMID:28368585]
ChEMBL	Inhibition of recombinant human HDAC10 after 60 mins by fluorescence assay	B	6.16	pIC50	686	nM	IC50	J Med Chem (2015) 58: 7672-7680 [PMID:26331334]
ChEMBL	Inhibition of human recombinant HDAC10 after 60 mins by fluorimetric assay	B	6.16	pIC50	686	nM	IC50	Eur J Med Chem (2014) 86: 639-652 [PMID:25218912]
ChEMBL	Inhibition of human HDAC10 using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	6.34	pIC50	460	nM	IC50	Eur J Med Chem (2016) 112: 99-105 [PMID:26890116]
ChEMBL	Inhibition of human HDAC-10 using RHKK(Ac) as substrate by fluorescence assay	B	6.34	pIC50	456	nM	IC50	J Med Chem (2014) 57: 2258-2274 [PMID:24588105]
ChEMBL	Inhibition of HDAC10 (unknown origin) using fluorogenic tetrapeptide RHKK(Ac) substrate by fluorescence assay	B	6.34	pIC50	456	nM	IC50	J Med Chem (2014) 57: 8358-8377 [PMID:25233084]
ChEMBL	Inhibition of human HDAC10 by fluorimetric assay	B	6.34	pIC50	456	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC-10 using RHKK(Ac) as substrate	B	6.34	pIC50	456	nM	IC50	Eur J Med Chem (2014) 76: 53-60 [PMID:24565573]
ChEMBL	Inhibition of human HDAC10 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.34	pIC50	456	nM	IC50	Bioorg Med Chem Lett (2014) 24: 61-64 [PMID:24345446]
ChEMBL	Inhibition of human HDAC10 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.34	pIC50	456	nM	IC50	Bioorg Med Chem Lett (2015) 25: 459-461 [PMID:25563890]
ChEMBL	Inhibition of human HDAC10 using fluorogenic tetrapeptide RHKKAc as substrate	B	6.34	pIC50	456	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of HDAC10 (unknown origin) using fluorogenic peptide from p53 residues (379 to 382) (RHKK(Ac)) as substrate by fluorescence assay	B	6.36	pIC50	432	nM	IC50	ACS Med Chem Lett (2013) 4: 779-783 [PMID:24015327]
ChEMBL	Inhibition of HDAC10 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	6.37	pIC50	430	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of HDAC10 (unknown origin) using fluorogenic peptide as substrate by fluorescence assay	B	6.37	pIC50	430	nM	IC50	J Med Chem (2014) 57: 8026-8034 [PMID:25238284]
ChEMBL	Inhibition of HDAC10 (unknown origin) using biotinylated histone H3 KAc peptide (1 to 21 residues) as substrate by HTRF assay	B	6.56	pIC50	278	nM	IC50	J Med Chem (2020) 63: 3678-3700 [PMID:32153186]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	6.62	pIC50	238.1	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HDAC10	B	6.68	pIC50	210	nM	IC50	Eur J Med Chem (2010) 45: 4331-4338 [PMID:20637529]
ChEMBL	Inhibition of recombinant N-terminal FLAG-tagged human HDAC10 (2 to 631 residues) expressed in baculovirus infected Sf9 cells using Ac-peptide-AMC as substrate after 1 hr by fluorescence assay	B	6.68	pIC50	208	nM	IC50	Eur J Med Chem (2019) 178: 116-130 [PMID:31177073]
ChEMBL	Inhibition of HDAC10 by in vitro deacetylation assay	B	6.7	pIC50	200	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of tubastatin-Alexa647-tracer binding to recombinant GST-tagged HDAC10 (unknown origin) measured after 1 hr by TR-FRET assay	B	6.7	pIC50	199.53	nM	IC50	J Med Chem (2019) 62: 4426-4443 [PMID:30964290]
ChEMBL	Inhibition of human recombinant HDAC10 using fluorogenic p53 (379-382 residues) (RHKK(Ac)AMC) as substrate	B	6.7	pIC50	198	nM	IC50	Eur J Med Chem (2023) 247: 115022-115022 [PMID:36549114]
ChEMBL	Inhibition of recombinant HDAC10 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	6.77	pIC50	170	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of recombinant full length N-terminal FLAG-tagged human HDAC10 expressed in baculovirus infected sf9 cells using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	6.77	pIC50	170	nM	IC50	J Med Chem (2019) 62: 1577-1592 [PMID:30629434]
ChEMBL	Inhibition of HDAC10 (unknown origin) incubated for 1 hr by TR-FRET analysis	B	6.8	pIC50	159	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of human HDAC10 using ArgHisLysLys(Ac) fluorogenic peptide as a substrate by fluorimetric assay	B	6.82	pIC50	150	nM	IC50	J Med Chem (2011) 54: 2165-2182 [PMID:21417297]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	6.84	pIC50	144.6	nM	IC50	J Med Chem (2023) 66: 5753-5773 [PMID:37057760]
ChEMBL	Inhibition of recombinant his-tagged human HDAC10 using Ac-ArgHisLys (Ac) Lys (Ac)-AMC substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate reader analysis	B	7	pIC50	100	nM	IC50	J Med Chem (2022) 65: 12200-12218 [PMID:36097406]
ChEMBL	Inhibition of HDAC10 (unknown origin) by fluorescence assay	B	7.01	pIC50	97	nM	IC50	Eur J Med Chem (2019) 164: 263-272 [PMID:30597327]
ChEMBL	Inhibition of HDAC10 (unknown origin) using (FAM)-labeled acetylated peptide as substrate measured after 17 hrs by fluorescence assay	B	7.04	pIC50	91.8	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	7.06	pIC50	88	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC10 (unknown origin) using Ac-ArgThr- Lys(Ac)Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	7.1	pIC50	80	nM	IC50	ACS Med Chem Lett (2019) 10: 1122-1127 [PMID:31413795]
ChEMBL	Inhibition of human recombinant HDAC10	B	7.1	pIC50	80	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	7.11	pIC50	77	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of recombinant human N-terminal FLAG-tagged HDAC10 (2 to 631 residues) expressed in baculovirus infected using 7-AMC-labelled HDAC substrate measured after 30 mins by fluorescence assay	B	7.11	pIC50	77	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human recombinant HDAC10	B	7.14	pIC50	72	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3023-3026 [PMID:19419863]
ChEMBL	Inhibition of N-terminal GST-tagged human HDAC10 (1 to 481 residues) using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	7.14	pIC50	72	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Inhibition of HDAC10 after 17 hrs	B	7.14	pIC50	72	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of HDAC10 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	7.14	pIC50	72	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of recombinant HDAC10 (unknown origin) using Fluor-de-Lys-SIRT1 as substrate by fluorescence assay	B	7.15	pIC50	70	nM	IC50	J Nat Prod (2014) 77: 49-56 [PMID:24328302]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	7.16	pIC50	68.4	nM	IC50	J Med Chem (2019) 62: 3171-3183 [PMID:30418766]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	7.19	pIC50	64	nM	IC50	Eur J Med Chem (2016) 108: 274-286 [PMID:26689485]
ChEMBL	Inhibition of recombinant human HDAC10 using Fluor-de-Lys as substrate after 30 mins by spectrophotometry	B	7.24	pIC50	58	nM	IC50	ACS Med Chem Lett (2011) 2: 703-707 [PMID:21984958]
ChEMBL	Inhibition of recombinant human C-terminal His-tagged HDAC10 expressed in baculovirus infected Sf9 insect cells using Ac-Arg-His-Lys(Ac)-Lys(Ac)-AMC as substrate measured after 30 mins by fluorescence assay	B	7.24	pIC50	57.25	nM	IC50	Eur J Med Chem (2020) 205: 112648-112648 [PMID:32791401]
ChEMBL	Inhibition of human recombinant HDAC10 using Ac-Leu-Gly-Lys(Ac)-AMC as substrate by measuring fluorescence intensity incubated for 30 mins by microplate reader assay	B	7.3	pIC50	50	nM	IC50	Eur J Med Chem (2021) 217: 113363-113363 [PMID:33744687]
ChEMBL	Inhibition of recombinant HDAC10 (unknown origin) using Ac-peptide-AMC as substrate incubated for 240 mins by microplate reader analysis	B	7.32	pIC50	47.5	nM	IC50	J Med Chem (2022) 65: 1243-1264 [PMID:33586434]
ChEMBL	Inhibition of N-terminal FLAG-tagged human HDAC10 (2 to 631 residues) expressed in baculovirus infected Sf9 cells using fluorogenic HDAC substrate 3 measured after 30 mins by fluorescence based assay	B	7.57	pIC50	27	nM	IC50	J Med Chem (2018) 61: 1552-1575 [PMID:29360358]
histone deacetylase 11/Histone deacetylase 11 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2]
ChEMBL	Inhibition of human recombinant HDAC11 using Ac-Leu-Gly-Lys (Ac)-AMC substrate incubated for 30 mins by fluorescence assay	B	7.22	pKi	60	nM	Ki	Medchemcomm (2014) 5: 1849-1855
GtoPdb	-	-	7.44	pKi	36	nM	Ki	Bioorg Med Chem Lett (2010) 20: 3314-21 [PMID:20451378]
ChEMBL	Inhibition of HDAC11	B	7.44	pKi	36	nM	Ki	Bioorg Med Chem Lett (2010) 20: 3314-3321 [PMID:20451378]
ChEMBL	Competitive inhibition of HDAC11 using KI-104 as substrate by fluorescence assay	B	7.51	pKi	31	nM	Ki	J Med Chem (2011) 54: 4694-4720 [PMID:21634430]
ChEMBL	Inhibition of recombinant human HDAC11 enzyme using Ac-LeuGlyLys(Ac)-AMC as substrate after 15 to 30 mins	B	7.89	pKi	13	nM	Ki	J Med Chem (2013) 56: 6512-6520 [PMID:23865683]
ChEMBL	Inhibition of human recombinant HDAC11 using [Ac-Leu-Gly-Lys(Ac)-AMC substrate incubated for 15 to 30 mins	B	7.89	pKi	13	nM	Ki	J Med Chem (2014) 57: 9644-9657 [PMID:25380299]
ChEMBL	Inhibition of recombinant his-tagged human HDAC11 using Ac-LeuGlyLys (Ac) AMC substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate reader analysis	B	4	pIC50	>100000	nM	IC50	J Med Chem (2022) 65: 12200-12218 [PMID:36097406]
ChEMBL	Inhibition of human recombinant HDAC11 using fluorogenic HDAC substrate class 2A by fluorescence assay	B	4.13	pIC50	73770	nM	IC50	Eur J Med Chem (2019) 164: 263-272 [PMID:30597327]
ChEMBL	Inhibition of HDAC11 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	4.4	pIC50	40000	nM	IC50	ACS Med Chem Lett (2019) 10: 1122-1127 [PMID:31413795]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	4.46	pIC50	34500	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of recombinant human HDAC11 using Boc-Lys(TFA)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence-based assay	B	4.9	pIC50	12542	nM	IC50	Eur J Med Chem (2016) 109: 350-359 [PMID:26814680]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	5	pIC50	>10000	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of recombinant human HDAC11 after 60 mins by fluorescence assay	B	5	pIC50	>10000	nM	IC50	J Med Chem (2015) 58: 7672-7680 [PMID:26331334]
ChEMBL	Inhibition of recombinant HDAC11 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	5	pIC50	>10000	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of HDAC 11 in human HeLa nuclear extract using fluorogenic HDAC class 2A substrate measured after 60 mins by fluorometric analysis	B	5	pIC50	>10000	nM	IC50	J Med Chem (2017) 60: 3484-3497 [PMID:28368585]
ChEMBL	Inhibition of HDAC11 (unknown origin) using biotinylated histone H3 KAc peptide (1 to 21 residues) as substrate by HTRF assay	B	5	pIC50	>10000	nM	IC50	J Med Chem (2020) 63: 3678-3700 [PMID:32153186]
ChEMBL	Inhibition of recombinant full length human HDAC11 expressed in baculovirus infected Sf9 cells using Ac-peptide-AMC as substrate after 1 hr by fluorescence assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2019) 178: 116-130 [PMID:31177073]
ChEMBL	Inhibition of HDAC11 (unknown origin) by fluorescence based assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112868-112868 [PMID:33077265]
ChEMBL	Inhibition of recombinant human full length HDAC11 expressed in baculovirus infected Sf9 cells measured after 60 mins by FRET assay	B	5	pIC50	>10000	nM	IC50	J Med Chem (2021) 64: 4020-4033 [PMID:33745280]
ChEMBL	Inhibition of HDAC11 (unknown origin) using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition by fluorescence based microplate reader analysis	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 220: 113453-113453 [PMID:33957387]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	5	pIC50	>10000	nM	IC50	J Med Chem (2023) 66: 5753-5773 [PMID:37057760]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	5.06	pIC50	8700	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of recombinant full-length human HDAC11 expressed in baculovirus infected Sf9 insect cells using 7-AMC-labelled HDAC substrate measured after 30 mins by fluorescence assay	B	5.06	pIC50	8700	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human recombinant HDAC11 after 60 mins by fluorimetric assay	B	5.23	pIC50	5840	nM	IC50	Eur J Med Chem (2014) 86: 639-652 [PMID:25218912]
ChEMBL	Inhibition of HDAC11 (unknown origin) measured by fluorescence based assay	B	5.3	pIC50	>5000	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
ChEMBL	Inhibition of HDAC11 (unknown origin) using Ac-ETDKrnyr-AMC as substrate incubated for 30 mins by fluorescence based analysis	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2023) 66: 14150-14174 [PMID:37796543]
ChEMBL	Inhibition of recombinant human HDAC11 measured after 30 mins by fluorescence microplate reader assay	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2021) 64: 15280-15296 [PMID:34624191]
ChEMBL	Inhibition of recombinant full length human HDAC11 expressed in baculovirus infected Sf9 cells using FAM-RHKK-TFAc as substrate incubated for 17 hrs by electrophoretic mobility shift assay	B	6	pIC50	>1000	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of recombinant full length N-terminal GST-tagged human HDAC11 expressed in baculovirus expression system using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	6	pIC50	>1000	nM	IC50	J Med Chem (2019) 62: 1577-1592 [PMID:30629434]
ChEMBL	Inhibition of recombinant full length HDAC11 (unknown origin) expressed in insect Sf9 cells by EMSA analysis	B	6	pIC50	>1000	nM	IC50	J Med Chem (2021) 64: 8486-8509 [PMID:34101461]
ChEMBL	Inhibition of full length human recombinant HDAC11 expressed in Sf9 baculovirus system using FAM-labeled acetylated peptide as substrate by measuring fluorescence intensity by EMSA method	B	6	pIC50	>1000	nM	IC50	J Med Chem (2022) 65: 3193-3217 [PMID:35119267]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	6	pIC50	>1000	nM	IC50	Bioorg Med Chem Lett (2021) 49: 128286-128286 [PMID:34314844]
ChEMBL	Inhibition of recombinant human N-terminal His-tagged HDAC11 expressed in baculovirus infected Sf9 insect cells using Ac-Arg-His-Lys(Ac)-Lys(Ac)-AMC as substrate measured after 30 mins by fluorescence assay	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2020) 205: 112648-112648 [PMID:32791401]
ChEMBL	Inhibition of HDAC11 (unknown origin) using Fluor de lys as substrate by fluorescence based analysis	B	6.02	pIC50	952	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of human HDAC11 by fluorescence assay	B	6.32	pIC50	480	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of His-tagged human HDAC11 expressed in Sf9 cells using Ac-Arg-Gly-Lys(Ac)-AMC as substrate by fluorometric assay	B	6.43	pIC50	370	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2434-2437 [PMID:27055940]
ChEMBL	Inhibition of human HDAC11 using fluorogenic tetrapeptide RHKKAc as substrate	B	6.44	pIC50	362	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC11 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.44	pIC50	362	nM	IC50	Bioorg Med Chem Lett (2014) 24: 61-64 [PMID:24345446]
ChEMBL	Inhibition of HDAC11 (unknown origin) using fluorogenic tetrapeptide RHKK(Ac) substrate by fluorescence assay	B	6.44	pIC50	362	nM	IC50	J Med Chem (2014) 57: 8358-8377 [PMID:25233084]
ChEMBL	Inhibition of human HDAC11 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.44	pIC50	362	nM	IC50	Bioorg Med Chem Lett (2015) 25: 459-461 [PMID:25563890]
ChEMBL	Inhibition of human HDAC-11 using RHKK(Ac) as substrate	B	6.44	pIC50	362	nM	IC50	Eur J Med Chem (2014) 76: 53-60 [PMID:24565573]
ChEMBL	Inhibition of human HDAC-11 using RHKK(Ac) as substrate by fluorescence assay	B	6.44	pIC50	362	nM	IC50	J Med Chem (2014) 57: 2258-2274 [PMID:24588105]
ChEMBL	Inhibition of human HDAC11 by fluorimetric assay	B	6.44	pIC50	362	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human recombinant N-terminal His-tagged HDAC11 (1 to 347 residues) using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	6.44	pIC50	360	nM	IC50	Eur J Med Chem (2016) 112: 99-105 [PMID:26890116]
ChEMBL	Inhibition of HDAC11 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of recombinant C-terminal His-tagged HDAC11 (unknown origin) expressed in baculovirus infected Sf9 cells using Ac-Arg-Gly-Lys(Ac)-AMC as substrate pretreated for 3 hrs followed by substrate addition after 30 mins by fluorometric method	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem Lett (2017) 27: 2943-2945 [PMID:28501514]
ChEMBL	Inhibition of HDAC11 (unknown origin) using fluorogenic peptide from p53 residues (379 to 382) (RHKK(Ac)) as substrate by fluorescence assay	B	6.7	pIC50	200	nM	IC50	ACS Med Chem Lett (2013) 4: 779-783 [PMID:24015327]
ChEMBL	Inhibition of HDAC11 (unknown origin) using fluorogenic peptide as substrate by fluorescence assay	B	6.7	pIC50	200	nM	IC50	J Med Chem (2014) 57: 8026-8034 [PMID:25238284]
ChEMBL	Inhibition of recombinant human HDAC11 using Fluor-de-Lys as substrate after 30 mins by spectrophotometry	B	6.88	pIC50	133	nM	IC50	ACS Med Chem Lett (2011) 2: 703-707 [PMID:21984958]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	6.96	pIC50	109	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of human HDAC11 using ArgHisLysLys(Ac) fluorogenic peptide as a substrate by fluorimetric assay	B	7	pIC50	100	nM	IC50	J Med Chem (2011) 54: 2165-2182 [PMID:21417297]
ChEMBL	Inhibition of recombinant HDAC11 (unknown origin) using Fluor-de-Lys-SIRT1 as substrate by fluorescence assay	B	7.05	pIC50	90	nM	IC50	J Nat Prod (2014) 77: 49-56 [PMID:24328302]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	7.21	pIC50	61.16	nM	IC50	Bioorg Med Chem Lett (2023) 88: 129305-129305 [PMID:37116762]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	7.29	pIC50	51.3	nM	IC50	J Med Chem (2019) 62: 3171-3183 [PMID:30418766]
ChEMBL	Inhibition of full length human recombinant HDAC11 expressed in baculovirus infected insect S9 cells after 30 mins by fluorescence assay	B	7.46	pIC50	34.5	nM	IC50	J Med Chem (2015) 58: 4325-4338 [PMID:25906087]
ChEMBL	Inhibition of human recombinant HDAC11	B	7.52	pIC50	30	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of human recombinant HDAC11 using fluorogenic class IIa (Bos-Lys(trifluoroacetyl)-AMC) as substrate	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2023) 247: 115022-115022 [PMID:36549114]
histone deacetylase 2/Histone deacetylase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769]
ChEMBL	Binding affinity to C-terminal His-tagged human HDAC2 (2 to 488 residues) in transfected in baculovirus infected Sf9 cells assessed as dissociation constant	B	6.79	pKd	163	nM	Kd	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of C-terminal GST-tagged recombinant human HDAC2 (1 to 488 residues) expressed in Baculovirus infected insect cells using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and further incubation for 90 mins measured after 15 mins by microplate reader based fluorescence assay	B	7	pKi	100	nM	Ki	Bioorg Med Chem (2019) 27: 115036-115036 [PMID:31431326]
ChEMBL	Inhibition of recombinant human HDAC2	B	7.05	pKi	90	nM	Ki	Bioorg Med Chem (2020) 28: 115108-115108 [PMID:31787463]
ChEMBL	Competitive inhibition of HDAC2 using KI-104 as substrate by fluorescence assay	B	7.38	pKi	42	nM	Ki	J Med Chem (2011) 54: 4694-4720 [PMID:21634430]
ChEMBL	Inhibition of HDAC2	B	7.4	pKi	40	nM	Ki	Bioorg Med Chem Lett (2010) 20: 3314-3321 [PMID:20451378]
ChEMBL	Binding affinity to recombinant human HDAC2 assessed as inhibition constant	B	7.82	pKi	15	nM	Ki	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of full-length recombinant human C-terminal GST tagged HDAC2 expressed in Sf9 insect cells assessed as inhibition constant using Ac-LGK(Ac)-AMC substrate and measured for 60 mins at 2 mins interval by fluorescence analysis	B	7.89	pKi	13	nM	Ki	J Med Chem (2023) 66: 15171-15188 [PMID:37847303]
ChEMBL	Inhibition of human recombinant HDAC2 using Ac-Leu-Gly-Lys (Ac)-AMC substrate incubated for 30 mins by fluorescence assay	B	8.1	pKi	8	nM	Ki	Medchemcomm (2014) 5: 1849-1855
ChEMBL	Inhibition of human recombinant HDAC2 using [Ac-Leu-Gly-Lys(Ac)-AMC substrate incubated for 15 to 30 mins	B	8.15	pKi	7	nM	Ki	J Med Chem (2014) 57: 9644-9657 [PMID:25380299]
ChEMBL	Inhibition of recombinant human HDAC2 enzyme using flurogenic Ac-LeuGlyLys (Ac)-AMC as substrate after 15 to 30 mins	B	8.15	pKi	7	nM	Ki	J Med Chem (2013) 56: 6512-6520 [PMID:23865683]
ChEMBL	Inhibition of His-tagged recombinant human HDAC2 expressed in insect cells assessed as hydrolase activity using fluorogenic AC-LGK-ac-AMC as substrate measured after 60 mins by fluorescence analysis	B	8.38	pKi	4.2	nM	Ki	Medchemcomm (2016) 7: 464-470
ChEMBL	Inhibition of human HDAC2	B	8.8	pKi	1.6	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
GtoPdb	-	-	8.8	pKi	1.6	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) incubated for 3 hrs by chemiluminescent assay	B	4.02	pIC50	96000	nM	IC50	Eur J Med Chem (2022) 244: 114807-114807 [PMID:36244186]
ChEMBL	Inhibition of human HDAC2 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.04	pIC50	921	nM	IC50	Bioorg Med Chem Lett (2014) 24: 61-64 [PMID:24345446]
ChEMBL	Inhibition of human HDAC2 using RHKK(Ac) as substrate by fluorimetric analysis	B	6.04	pIC50	921	nM	IC50	Bioorg Med Chem Lett (2015) 25: 459-461 [PMID:25563890]
ChEMBL	Inhibition of human HDAC-2 using RHKK(Ac) as substrate	B	6.04	pIC50	921	nM	IC50	Eur J Med Chem (2014) 76: 53-60 [PMID:24565573]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic tetrapeptide RHKK(Ac) substrate by fluorescence assay	B	6.04	pIC50	921	nM	IC50	J Med Chem (2014) 57: 8358-8377 [PMID:25233084]
ChEMBL	Inhibition of human HDAC2 by fluorimetric assay	B	6.04	pIC50	921	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.04	pIC50	920	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of human HDAC2 by fluorescence assay	B	6.04	pIC50	920	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of human recombinant HDAC2 using AMC-K(Ac)GL as substrate by fluorescence based assay	B	6.04	pIC50	920	nM	IC50	ACS Med Chem Lett (2020) 11: 977-983 [PMID:32435414]
ChEMBL	Inhibition of human recombinant full length HDAC2 using (Ac)Arg-Gly-Lys(Ac) as substrate after 60 mins by fluorescence assay	B	6.04	pIC50	920	nM	IC50	J Med Chem (2013) 56: 9934-9954 [PMID:24261862]
ChEMBL	Inhibition of human HDAC-2 using RHKK(Ac) as substrate by fluorescence assay	B	6.04	pIC50	920	nM	IC50	J Med Chem (2014) 57: 2258-2274 [PMID:24588105]
ChEMBL	Inhibition of HDAC2 by fluorometric assay	B	6.11	pIC50	780	nM	IC50	J Med Chem (2012) 55: 9562-9575 [PMID:23116147]
ChEMBL	Inhibition of recombinant human HDAC2 after 60 mins by fluorescence assay	B	6.12	pIC50	760	nM	IC50	J Med Chem (2015) 58: 7672-7680 [PMID:26331334]
ChEMBL	Inhibition of HDAC2 (unknown origin) by fluorimetric assay	B	6.26	pIC50	550	nM	IC50	Bioorg Med Chem Lett (2016) 26: 154-159 [PMID:26611919]
ChEMBL	Inhibition of C-terminal His-tagged full length human recombinant HDAC2 expressed in baculovirus expression system using fluorogenic acetylated peptide as substrate preincubated with enzyme for 10 mins prior to substrate addition measured after 60 mins by fluorescence analysis	B	6.27	pIC50	537	nM	IC50	ACS Med Chem Lett (2013) 4: 132-136 [PMID:24900575]
ChEMBL	Inhibition of HDAC 2 in human HeLa nuclear extract using HDAC substrate-3 measured after 60 mins by fluorometric analysis	B	6.34	pIC50	454	nM	IC50	J Med Chem (2017) 60: 3484-3497 [PMID:28368585]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic HDAC substrate-3 by fluorescence assay	B	6.39	pIC50	411	nM	IC50	Eur J Med Chem (2019) 164: 263-272 [PMID:30597327]
ChEMBL	Inhibition of HDAC2 (unknown origin) using RHKK(Ac) (379 to 382) p53 peptide as substrate by fluorescence assay	B	6.39	pIC50	410	nM	IC50	J Med Chem (2014) 57: 4009-4022 [PMID:24766560]
ChEMBL	Inhibition of HDAC2 by in vitro deacetylation assay	B	6.4	pIC50	400	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of HDAC2	B	6.41	pIC50	390	nM	IC50	Eur J Med Chem (2010) 45: 4331-4338 [PMID:20637529]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.44	pIC50	362	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of recombinant human HDAC2 using fluorogenic substrate by fluorescence assay	B	6.48	pIC50	332.65	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.49	pIC50	322	nM	IC50	J Med Chem (2023) 66: 5753-5773 [PMID:37057760]
ChEMBL	Inhibition of human recombinant HDAC2	B	6.55	pIC50	282	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3023-3026 [PMID:19419863]
ChEMBL	Inhibition of HDAC2 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	6.55	pIC50	282	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of HDAC2 after 17 hrs	B	6.55	pIC50	282	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) incubated for 10 mins using Boc-Lys(acetyl)-AMC fluorogenic substrate by homogeneous fluorescence release assay	B	6.55	pIC50	280	nM	IC50	Bioorg Med Chem (2015) 23: 3457-3471 [PMID:25953722]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using fluorogenic substrate Boc-Lys (acetyl)-AMC after 20 mins by homogeneous fluorescence release assay	B	6.55	pIC50	280	nM	IC50	Eur J Med Chem (2015) 96: 1-13 [PMID:25874326]
ChEMBL	Inhibition of human recombinant HDAC2 using Boc-L-Lys (Ac)-AMC as substrate preincubated with compound for 24 hrs measured after 35 mins by spectrofluorometric analysis	B	6.59	pIC50	260	nM	IC50	Bioorg Med Chem Lett (2012) 22: 5025-5030 [PMID:22771007]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated with enzyme for 5 mins prior to substrate addition measured after 30 mins by fluorescence assay	B	6.59	pIC50	256	nM	IC50	ACS Med Chem Lett (2013) 4: 235-238 [PMID:23493449]
ChEMBL	Inhibition of human recombinant HDAC2 by fluorimetry	B	6.6	pIC50	250	nM	IC50	J Med Chem (2009) 52: 2909-2922 [PMID:19419205]
ChEMBL	Inhibition of human recombinant HDAC2 using Tosyl-Gly-ProLys(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	6.62	pIC50	240	nM	IC50	J Med Chem (2021) 64: 10403-10417 [PMID:34185525]
ChEMBL	Inhibition of human HDAC2 using ArgHisLysLys(Ac) fluorogenic peptide as a substrate by fluorimetric assay	B	6.62	pIC50	240	nM	IC50	J Med Chem (2011) 54: 2165-2182 [PMID:21417297]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic peptide as substrate by fluorescence assay	B	6.62	pIC50	240	nM	IC50	J Med Chem (2014) 57: 8026-8034 [PMID:25238284]
ChEMBL	Inhibition of HDAC2 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	6.62	pIC50	240	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic peptide from p53 residues (379 to 382) (RHKK(Ac)) as substrate by fluorescence assay	B	6.63	pIC50	232	nM	IC50	ACS Med Chem Lett (2013) 4: 779-783 [PMID:24015327]
ChEMBL	Inhibition of recombinant human full length C-terminal His- tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using fluorometric substrate measured after 60 mins by FRET assay	B	6.64	pIC50	230	nM	IC50	J Med Chem (2021) 64: 4020-4033 [PMID:33745280]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic Boc-Lys(acetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by fluorescence-based assay	B	6.66	pIC50	220	nM	IC50	J Med Chem (2018) 61: 5304-5322 [PMID:29787262]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	6.66	pIC50	220	nM	IC50	J Med Chem (2019) 62: 3898-3923 [PMID:30901208]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic HDAC substrate 3 incubated for 30 mins by fluorescence based assay	B	6.66	pIC50	220	nM	IC50	J Med Chem (2018) 61: 1552-1575 [PMID:29360358]
ChEMBL	Inhibition of full length recombinant human HDAC2 expressed in baculovirus infected Sf9 insect cells using KI 177 as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by fluorescence assay	B	6.67	pIC50	214	nM	IC50	Eur J Med Chem (2018) 143: 792-805 [PMID:29223096]
ChEMBL	Inhibition of HDAC2 (unknown origin) using (FAM)-labeled acetylated peptide as substrate measured after 17 hrs by fluorescence assay	B	6.67	pIC50	213	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC2 after 10 mins by fluorometric assay	B	6.68	pIC50	210	nM	IC50	Bioorg Med Chem (2010) 18: 4119-4137 [PMID:20452225]
ChEMBL	Inhibition of recombinant C-terminal FLAG-tagged HDAC2 expressed in baculovirus after 10 mins by fluorimetric analysis	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem Lett (2009) 19: 5684-5688 [PMID:19699639]
ChEMBL	Inhibition of recombinant human full-length HDAC2 expressed in baculovirus expression system using Boc-l-Lys(Ac)-AMC as substrate preincubated up to 24 hrs and measured after 60 mins by fluorescence assay	B	6.7	pIC50	200	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC2 (unknown origin) measured after 30 mins by fluorescence microplate reader assay	B	6.7	pIC50	200	nM	IC50	J Med Chem (2021) 64: 15280-15296 [PMID:34624191]
ChEMBL	Inhibition of full length human HDAC2 using Boc-Lys(acetyl)-AMC as fluorogenic substrate measured after 1 hr by flourescence plate reader method	B	6.7	pIC50	>200	nM	IC50	Eur J Med Chem (2021) 225: 113799-113799 [PMID:34500130]
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG-tagged HDAC2 expressed in baculovirus infected Sf9 insect cells using Boc-Lys(epsilon-Ac)-AMC as substrate measured after 90 mins by fluorescence assay	B	6.7	pIC50	200	nM	IC50	J Med Chem (2017) 60: 5493-5506 [PMID:28574690]
ChEMBL	Inhibition of human recombinant HDAC2 using Boc-L-Lys (Ac)-AMC as substrate preincubated with compound for 5 mins measured after 35 mins by spectrofluorometric analysis	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem Lett (2012) 22: 5025-5030 [PMID:22771007]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.71	pIC50	197	nM	IC50	Eur J Med Chem (2013) 64: 273-284 [PMID:23644210]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.71	pIC50	197	nM	IC50	Eur J Med Chem (2016) 108: 53-67 [PMID:26629860]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition by fluorescence assay	B	6.71	pIC50	193	nM	IC50	Medchemcomm (2015) 6: 1816-1825
ChEMBL	Inhibition of HDAC2 in human HeLa cells using BOC-K(Ac)-AMC as substrate measured after 30 mins by fluorescence based microtiter plate reader assay	B	6.73	pIC50	188	nM	IC50	J Med Chem (2022) 65: 11187-11213 [PMID:35926141]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	6.73	pIC50	184.7	nM	IC50	J Med Chem (2014) 57: 3324-3341 [PMID:24694055]
ChEMBL	Inhibition of recombinant human full length C-terminal Flag-tagged HDAC2 expressed in baculovirus infected Sf9 cells using ZMAL as substrate incubated for 90 mins by fluorometric method	B	6.74	pIC50	183	nM	IC50	Bioorg Med Chem (2019) 27: 115039-115039 [PMID:31420257]
ChEMBL	Inhibition of human recombinant HDAC2 after 60 mins by fluorimetric assay	B	6.74	pIC50	182	nM	IC50	Eur J Med Chem (2014) 86: 639-652 [PMID:25218912]
ChEMBL	Inhibition of recombinant human HDAC2 preincubated with enzyme followed by flour de lys-green substrate addition measured after 1 hr by fluorescence assay	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4415-4420 [PMID:28818449]
ChEMBL	Inhibition of HDAC2 in human HeLa nuclear extract using Boc-Lys(acetyl)-AMC as substrate measured after 30 mins by fluorescence assay	B	6.74	pIC50	180	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of recombinant HDAC2 using Ac-Lys(Ac)-AMC as substrate after 30 mins by fluorescence analysis	B	6.74	pIC50	180	nM	IC50	ACS Med Chem Lett (2012) 3: 505-508 [PMID:24900500]
ChEMBL	Inhibition of human recombinant HDAC2 using Fluor de Lys-Green as substrate preincubated for 5 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	6.74	pIC50	180	nM	IC50	Eur J Med Chem (2018) 143: 320-333 [PMID:29202397]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.75	pIC50	178	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127128-127128 [PMID:32247729]
ChEMBL	Inhibition of human HDAC2 assessed as target activity using fluorogenic substrate ZMAL for 90 min by microplate reader at 355nm	B	6.75	pIC50	178	nM	IC50	J Med Chem (2021) 64: 14620-14646 [PMID:34582215]
ChEMBL	Inhibition of HDAC2 (unknown origin) pre-incubated for 5 mins before fluorogenic substrate Boc-Lys (acetyl)-AMC or Boc-Lys (triflouroacetyl)-AMC addition and measured after 30 mins by fluorescence based assay	B	6.75	pIC50	176	nM	IC50	J Med Chem (2020) 63: 4701-4715 [PMID:32267687]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.76	pIC50	174.6	nM	IC50	Eur J Med Chem (2015) 89: 628-637 [PMID:25462271]
ChEMBL	Inhibition of recombinant C-terminal His-tagged full length human HDAC2 expressed in baculovirus infected insect Sf9 cells using fluorogenic HDAC substrate 3 after 30 mins by fluorescence assay	B	6.76	pIC50	174	nM	IC50	ACS Med Chem Lett (2015) 6: 239-243 [PMID:25815139]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) measured after 30 mins by fluorescence assay	B	6.76	pIC50	173	nM	IC50	Eur J Med Chem (2019) 180: 171-190 [PMID:31306905]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.77	pIC50	170	nM	IC50	Eur J Med Chem (2021) 219: 113419-113419 [PMID:33845233]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.77	pIC50	170	nM	IC50	J Med Chem (2021) 64: 3392-3426 [PMID:33661013]
ChEMBL	Inhibition of HDAC2	B	6.77	pIC50	170	nM	IC50	Bioorg Med Chem Lett (2008) 18: 726-731 [PMID:18060775]
ChEMBL	Inhibition of C-terminal GST-tagged full-length recombinant human HDAC2 expressed in baculovirus infected Sf9 insect cells using Ac-Leu-Gly-Lys(Ac)-AMC as substrate incubated for 30 mins by fluorescence based assay	B	6.77	pIC50	168	nM	IC50	J Med Chem (2023) 66: 1239-1253 [PMID:36622852]
ChEMBL	Inhibition of HDAC2 (unknown origin) incubated for 30 mins by microplate reader assay	B	6.78	pIC50	167	nM	IC50	Eur J Med Chem (2022) 229: 114058-114058 [PMID:34954595]
ChEMBL	Inhibition of recombinant human HDAC2 expressed in baculovirus infected Sf9 cells using Boc-Lys(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	6.78	pIC50	166	nM	IC50	Eur J Med Chem (2020) 188: 111991-111991 [PMID:31883490]
ChEMBL	Inhibition of HDAC2	B	6.79	pIC50	164	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of human recombinant HDAC2 using fluorogenic substrate measured after 30 mins	B	6.79	pIC50	164	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of full length C-terminal 6x-His tagged human HDAC2 using Arg-His-Lys-Lys(Ac) substrate incubated for 2 hrs by fluorescence assay	B	6.79	pIC50	164	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5450-5454 [PMID:25454270]
ChEMBL	Inhibition of human recombinant HDAC2	B	6.79	pIC50	163.6	nM	IC50	Eur J Med Chem (2009) 44: 1067-1085 [PMID:18672316]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.8	pIC50	160	nM	IC50	Bioorg Med Chem Lett (2022) 71: 128821-128821 [PMID:35643262]
ChEMBL	Inhibition of full length C-terminal FLAG-tagged human recombinant HDAC2 (1 to 488 residues) expressed in Sf9 cells using ZMAL as fluorogenic substrate incubated for 90 mins by microplate reader analysis	B	6.8	pIC50	160	nM	IC50	J Med Chem (2023) 66: 14787-14814 [PMID:37902787]
ChEMBL	Inhibition of C-terminal 6His-tagged human recombinant HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate preincubated for 60 mins followed by substrate addition and measured after 90 mins by microplate reader analysis	B	6.8	pIC50	158	nM	IC50	J Med Chem (2023) 66: 13821-13837 [PMID:37782298]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.81	pIC50	156	nM	IC50	J Med Chem (2019) 62: 3171-3183 [PMID:30418766]
ChEMBL	Inhibition of HDAC2 (unknown origin) preincubated for 60 mins	B	6.81	pIC50	154.6	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HDAC2 (unknown origin) after 30 mins by fluorescence assay	B	6.82	pIC50	153.1	nM	IC50	J Med Chem (2018) 61: 7245-7260 [PMID:30045621]
ChEMBL	Inhibition of human HDAC2 after 40 mins by fluorescence analysis	B	6.82	pIC50	150	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4457-4460 [PMID:26376355]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins by fluorescence assay	B	6.83	pIC50	149	nM	IC50	J Med Chem (2017) 60: 7965-7983 [PMID:28885834]
ChEMBL	Inhibition of C-terminal flag tagged human recombinant full length HDAC2 (1 to 488 residues) using Z- Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins followed by trypsin addition and measured after 30 min by microplate reader analysis	B	6.83	pIC50	147.2	nM	IC50	ACS Med Chem Lett (2023) 14: 1863-1868 [PMID:38116436]
ChEMBL	Inhibition of recombinant human full length C-terminal FLAG-tagged HDAC2 expressed in baculovirus infected Sf9 insect cells using Boc-Lys(epsilon-acetyl)-AMC as substrate incubated for 90 mins by fluorescence assay	B	6.84	pIC50	146	nM	IC50	J Med Chem (2019) 62: 11260-11279 [PMID:31762274]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.85	pIC50	140	nM	IC50	Eur J Med Chem (2016) 121: 451-483 [PMID:27318122]
ChEMBL	Inhibition of recombinant human full-length HDAC2 expressed in baculovirus expression system using 7-AMC-labelled HDAC substrate measured after 30 mins by fluorescence assay	B	6.85	pIC50	140	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.85	pIC50	140	nM	IC50	Bioorg Med Chem Lett (2017) 27: 3289-3293 [PMID:28648462]
ChEMBL	Inhibition of HDAC2 (unknown origin) preincubated for 90 mins	B	6.86	pIC50	137.2	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of recombinant human HDAC2 using Boc-Lys(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence-based assay	B	6.87	pIC50	135	nM	IC50	Eur J Med Chem (2016) 109: 350-359 [PMID:26814680]
ChEMBL	Inhibition of HDAC2 (unknown origin) in using Boc-Lys(acetyl)-AMC or Boc-Lys (triflouroacetyl)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	6.89	pIC50	128.9	nM	IC50	J Med Chem (2020) 63: 12083-12099 [PMID:33021789]
ChEMBL	Inhibition of HDAC2 (unknown origin) preincubated for 120 mins	B	6.91	pIC50	123.7	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.92	pIC50	120.75	nM	IC50	Bioorg Med Chem Lett (2023) 88: 129305-129305 [PMID:37116762]
ChEMBL	Inhibition of full length human recombinant HDAC2 expressed in baculovirus infected insect S9 cells using Ac-Leu-GlyLys(Ac)-AMC as substrate after 30 mins by fluorescence assay	B	6.92	pIC50	120.5	nM	IC50	J Med Chem (2015) 58: 4325-4338 [PMID:25906087]
ChEMBL	Inhibition of full length recombinant human HDAC2 expressed in baculovirus expression system assessed as release of 7-amino-4-methylcoumarin by fluorogenic assay	B	6.92	pIC50	120	nM	IC50	Eur J Med Chem (2016) 122: 92-101 [PMID:27344487]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	6.92	pIC50	120	nM	IC50	ACS Med Chem Lett (2019) 10: 1122-1127 [PMID:31413795]
ChEMBL	Inhibition of recombinant human full length HDAC2 using Fluor-de-Lys as substrate after 60 mins by spectrofluorimetric analysis	B	6.92	pIC50	120	nM	IC50	Eur J Med Chem (2017) 134: 13-23 [PMID:28395150]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	6.93	pIC50	118	nM	IC50	Eur J Med Chem (2016) 108: 274-286 [PMID:26689485]
ChEMBL	Inhibition of human HDAC2 using Boc-Lys (acetyl)-AMC as substrate pretreated with compound for 1 hrs followed by substrate addition measured after 1 hrs by microplate reader analysis	B	6.93	pIC50	116.27	nM	IC50	Eur J Med Chem (2022) 243: 114705-114705 [PMID:36215854]
ChEMBL	Inhibition of full length recombinant his-tagged human HDAC2 using Ac-LeuGlyLys (Ac) AMC substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate reader analysis	B	6.94	pIC50	114	nM	IC50	J Med Chem (2022) 65: 12200-12218 [PMID:36097406]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Ac-LeuGlyLys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition and further incubated for incubated for 1 hrs by fluorescence microtiter plate reader assay	B	6.95	pIC50	112.5	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using Fluor-de-Lys-SIRT1 as substrate by fluorescence assay	B	6.96	pIC50	110	nM	IC50	J Nat Prod (2014) 77: 49-56 [PMID:24328302]
ChEMBL	Inhibition of recombinant human full length C-terminal GST-fusion tagged HDAC2 expressed in baculovirus infected insect cells using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition measured after 90 mins by fluorimetry	B	6.97	pIC50	107	nM	IC50	Eur J Med Chem (2021) 211: 113095-113095 [PMID:33360560]
ChEMBL	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	B	7	pIC50	100.9	nM	IC50	US-9353061-B2. 3,5,N-trihydroxy-alkanamide and derivatives: method for making same and use thereof (2016)
ChEMBL	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	B	7	pIC50	100.9	nM	IC50	US-9115116-B2. Dual action inhibitors against histone deacetylases and 3-hydroxy-3-methylglutaryl coenzyme a reductase (2015)
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) after 10 mins by fluorimetric analysis	B	7	pIC50	100.9	nM	IC50	J Med Chem (2013) 56: 3645-3655 [PMID:23570542]
ChEMBL	Inhibition of HDAC2 in human HeLa cells using KI-104 as substrate after 40 mins by fluorescence analysis	B	7	pIC50	100	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human recombinant HDAC2 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	7	pIC50	100	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of HDAC2 in human HeLa cells	B	7	pIC50	100	nM	IC50	Eur J Med Chem (2009) 44: 1900-1912 [PMID:19084294]
ChEMBL	Inhibition of human recombinant HDAC2 using Fluor de Lys Green as substrate incubated for 30 mins by fluorescence assay	B	7	pIC50	100	nM	IC50	Eur J Med Chem (2016) 112: 99-105 [PMID:26890116]
ChEMBL	Inhibition of HDAC2 (unknown origin) preincubated for 30 mins	B	7.01	pIC50	98.47	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of HADC2 (unknown origin)	B	7.02	pIC50	96	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of full length C-terminal his6-tagged human recombinant HDAC2 (1 to 488 residues)	B	7.02	pIC50	96	nM	IC50	J Med Chem (2022) 65: 14764-14791 [PMID:36306372]
ChEMBL	Inhibition of HDAC2 in human HeLa-S3 cell lysates preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 40 mins by fluorimetric method	B	7.02	pIC50	96	nM	IC50	Eur J Med Chem (2018) 143: 1790-1806 [PMID:29150330]
ChEMBL	Inhibition of HDAC2 in human HeLaS3 cells preincubated for 15 mins followed by HDAC-Glo substrate addition measured after 30 to 45 mins by ELISA	B	7.02	pIC50	96	nM	IC50	ACS Med Chem Lett (2017) 8: 281-286 [PMID:28337317]
ChEMBL	Inhibition of HDAC2 (unknown origin) by ELISA-based assay	B	7.02	pIC50	96	nM	IC50	Bioorg Med Chem Lett (2017) 27: 3254-3258 [PMID:28648461]
ChEMBL	Inhibition of full length human C-terminal His-tagged HDAC2 using fluorogenic acetylated peptide as substrate incubated for 30 mins by fluorescence plate reader assay	B	7.03	pIC50	93.6	nM	IC50	J Med Chem (2021) 64: 3392-3426 [PMID:33661013]
ChEMBL	Inhibition of HDAC2 (unknown origin) preincubated for 5 mins	B	7.03	pIC50	93.22	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of human recombinant full-length C-terminal GST-tagged HDAC2 expressed in baculovirus infected Sf9 cells using Boc-Lys(acetyl)-AMC as substrate after 30 mins by fluorescence assay	B	7.04	pIC50	91.6	nM	IC50	Eur J Med Chem (2017) 141: 596-602 [PMID:29102179]
ChEMBL	Inhibition of recombinant human C-terminal His-tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using fluor de lys SIRT1 as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	7.04	pIC50	91	nM	IC50	Eur J Med Chem (2020) 192: 112193-112193 [PMID:32151835]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.05	pIC50	90	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3346-3348 [PMID:23591111]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.05	pIC50	89.23	nM	IC50	Bioorg Med Chem Lett (2022) 70: 128797-128797 [PMID:35580726]
ChEMBL	Inhibition of recombinant human HDAC2 expressed in baculovirus expression system using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based micro plate reader analysis	B	7.05	pIC50	89.23	nM	IC50	J Med Chem (2020) 63: 5501-5525 [PMID:32321249]
ChEMBL	Inhibition of human recombinant HDAC2 after 30 mins by fluorescence assay	B	7.07	pIC50	85.8	nM	IC50	J Nat Prod (2013) 76: 2026-2033 [PMID:24164245]
ChEMBL	Inhibition of C-terminal FLAG tagged HDAC2	B	7.09	pIC50	82	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3081-3084 [PMID:19410459]
ChEMBL	Inhibition of HDAC2	B	7.09	pIC50	82	nM	IC50	J Med Chem (2008) 51: 2350-2353 [PMID:18370373]
ChEMBL	Inhibition of human C-terminal FLAG-tagged HDAC2 in HEK293 cells	B	7.09	pIC50	82	nM	IC50	J Med Chem (2009) 52: 3453-3456 [PMID:19441846]
ChEMBL	Inhibition of HDAC2	B	7.09	pIC50	82	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5528-5532 [PMID:18809328]
ChEMBL	Inhibition of human full-length C-terminal FLAG-tagged HDAC2 expressed in baculovirus infected Sf9 insect cells using Ac-Leu-Gly-Lys(Ac)-AMC as substrate measured after 30 mins by fluorescence assay	B	7.09	pIC50	81.28	nM	IC50	Eur J Med Chem (2020) 205: 112648-112648 [PMID:32791401]
ChEMBL	Inhibition of C-terminal GST-tagged human recombinant HDAC2 expressed in Sf9 cells after 30 mins by microtiter plate reader analysis	B	7.09	pIC50	81	nM	IC50	Bioorg Med Chem (2016) 24: 1446-1454 [PMID:26907204]
ChEMBL	Inhibition of HDAC2 in human HeLa cells after 72 hrs by ELISA	B	7.09	pIC50	81	nM	IC50	Eur J Med Chem (2018) 146: 79-92 [PMID:29396364]
ChEMBL	Inhibition of human recombinant HDAC2 preincubated for 1 hr with substrate by protease coupled end-point assay	B	7.12	pIC50	76	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3142-3145 [PMID:20392638]
ChEMBL	Inhibition of Histone deacetylase 2 (HDAC2) activity of HeLa nuclear extracts	B	7.14	pIC50	73	nM	IC50	Bioorg Med Chem Lett (2004) 14: 449-453 [PMID:14698179]
ChEMBL	Inhibition of human recombinant HDAC2 preincubated for 24 hrs followed by 1 hr reaction with substrate by protease coupled end-point assay	B	7.14	pIC50	72	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3142-3145 [PMID:20392638]
ChEMBL	Inhibition of recombinant human C-terminal His-tagged HDAC2 (1 to end residues) expressed in sf9 insect cells using Boc-Lys(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	7.15	pIC50	70	nM	IC50	Eur J Med Chem (2020) 192: 112189-112189 [PMID:32151834]
ChEMBL	Inhibition of full length HDAC2 assessed as 7-amino-4-methylcoumarin release from fluorophore conjugated substrate after 5 mins by fluorescence assay	B	7.18	pIC50	66	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2601-2605 [PMID:21334896]
ChEMBL	Inhibition of recombinant human HDAC2 using Fluor-de-Lys as substrate after 30 mins by spectrophotometry	B	7.18	pIC50	66	nM	IC50	ACS Med Chem Lett (2011) 2: 703-707 [PMID:21984958]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.25	pIC50	56	nM	IC50	Medchemcomm (2014) 5: 1829-1833
ChEMBL	Inhibition of HDAC2 in human HeLa nuclear extract	B	7.26	pIC50	55	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of human recombinant HDAC2 using MAZ1600 as fluorogenic substrate measured every 5 mins by optimized homogenous fluorescence based assay	B	7.28	pIC50	52	nM	IC50	J Med Chem (2015) 58: 4812-4821 [PMID:25974739]
ChEMBL	Inhibition of recombinant full length human HDAC2 expressed in baculovirus infected Sf9 cells using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by fluorescence based assay	B	7.29	pIC50	51	nM	IC50	Eur J Med Chem (2019) 182: 111672-111672 [PMID:31505452]
ChEMBL	Inhibition of HDAC2 in HeLa cell lysates	B	7.3	pIC50	>=50	nM	IC50	Bioorg Med Chem Lett (2007) 17: 6261-6265 [PMID:17892933]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem (2023) 85: 117242-117242 [PMID:37079967]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.4	pIC50	40	nM	IC50	ACS Med Chem Lett (2018) 9: 34-38 [PMID:29348808]
ChEMBL	Inhibition of full-length recombinant human C-terminal GST tagged HDAC2 expressed in Sf9 insect cells using aminoluciferin peptide substrate preincubated for 3 hrs followed by substrate addition and measured after 30 mins by HDAC-Glo I/II chemiluminescence assay	B	7.42	pIC50	38	nM	IC50	J Med Chem (2023) 66: 15171-15188 [PMID:37847303]
ChEMBL	Inhibition of HDAC2	B	7.44	pIC50	36	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
ChEMBL	Inhibition of human full-length recombinant HDAC2 preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	7.46	pIC50	35	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of recombinant full length human HDAC2 expressed in baculovirus expression system using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence assay	B	7.46	pIC50	35	nM	IC50	J Med Chem (2019) 62: 1577-1592 [PMID:30629434]
ChEMBL	Inhibition of HDAC2 (unknown origin) using biotinylated histone H3 KAc peptide (1 to 21 residues) as substrate by HTRF assay	B	7.46	pIC50	35	nM	IC50	J Med Chem (2020) 63: 3678-3700 [PMID:32153186]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	7.46	pIC50	35	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.48	pIC50	33	nM	IC50	J Med Chem (2022) 65: 3080-3097 [PMID:35148101]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.48	pIC50	33	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127537-127537 [PMID:32916298]
ChEMBL	Inhibition of human recombinant HDAC2 using Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 30 mins followed by substrate addition and measured after 15 to 30 mins	B	7.49	pIC50	32	nM	IC50	J Med Chem (2018) 61: 3779-3798 [PMID:29039662]
ChEMBL	Inhibition of human recombinant HDAC2	B	7.49	pIC50	32	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of full length recombinant human C-terminal His-tagged HDAC2 expressed in baculovirus infected sf9 cells using Boc-Lys-(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	7.51	pIC50	31	nM	IC50	Bioorg Med Chem (2018) 26: 1418-1425 [PMID:29500130]
ChEMBL	Inhibition of recombinant full length human HDAC2 expressed in sf9 insect cells using Ac-peptide-AMC as substrate after 1 hr by fluorescence assay	B	7.54	pIC50	29	nM	IC50	Eur J Med Chem (2019) 178: 116-130 [PMID:31177073]
ChEMBL	Inhibition of human HDAC2 pre-incubated for 30 mins before substrate addition and measured after 30 mins by HDAC-Glo I/II assay	B	7.55	pIC50	28	nM	IC50	Bioorg Med Chem (2014) 22: 3720-3731 [PMID:24864038]
ChEMBL	Inhibition of C-terminal His-tagged full length human HDAC2 expressed in baculovirus expression system using Ac-peptide substrate incubated for 15 mins by fluorescence based assay	B	7.55	pIC50	28	nM	IC50	Eur J Med Chem (2017) 140: 392-402 [PMID:28987602]
ChEMBL	Inhibition of HDAC2 (unknown origin) by colorimetric method	B	7.55	pIC50	28	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.57	pIC50	27	nM	IC50	Eur J Med Chem (2021) 218: 113392-113392 [PMID:33831778]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Ac-peptide as a substrate pretreated for 15 mins followed by substrate addition	B	7.59	pIC50	26	nM	IC50	Eur J Med Chem (2021) 225: 113824-113824 [PMID:34509167]
ChEMBL	Inhibition of human recombinant HDAC2 using fluor de Lys as substrate by fluorometric analysis	B	7.62	pIC50	24	nM	IC50	J Med Chem (2010) 53: 8663-8678 [PMID:21080647]
ChEMBL	Inhibition of recombinant human full length C-terminal His tagged HDAC2 (1 to 488 residues) expressed in baculovirus-infected Sf9 insect cells using Ac-peptide as substrate incubated for 4 hrs	B	7.62	pIC50	23.8	nM	IC50	J Med Chem (2022) 65: 4156-4181 [PMID:35175762]
ChEMBL	Inhibition of HDAC2 (unknown origin) using trypsin and Ac-peptide as substrates	B	7.67	pIC50	21.61	nM	IC50	Eur J Med Chem (2022) 230: 114115-114115 [PMID:35033824]
ChEMBL	Inhibition of HDAC2 (unknown origin) assessed as release of fluorogenic AMC preincubated for 15 mins followed by addition of trypsin and substrate measured after 60 mins Spectra max microplate reader analysis	B	7.7	pIC50	20	nM	IC50	J Med Chem (2023) 66: 4802-4826 [PMID:36934335]
ChEMBL	Inhibition of human recombinant HDAC2 using fluorogenic p53 (379-382 residues) (RHKK(Ac)AMC) as substrate	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2023) 247: 115022-115022 [PMID:36549114]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Boc-Lys-(acetyl)-AMC as substrate incubated for 0.5 hr and measured after 20 mins by fluorescence assay	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2022) 233: 114228-114228 [PMID:35245830]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.73	pIC50	18.55	nM	IC50	Eur J Med Chem (2020) 205: 112679-112679 [PMID:32791404]
ChEMBL	Inhibition of C-terminal 6His-tagged human recombinant HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition by fluorescence based analysis	B	7.73	pIC50	18.42	nM	IC50	Eur J Med Chem (2023) 254: 115367-115367 [PMID:37086699]
ChEMBL	Inhibition of HDAC2 (unknown origin) after 60 mins by fluorescence assay	B	7.74	pIC50	18	nM	IC50	J Med Chem (2013) 56: 4816-4820 [PMID:23672185]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Ac-peptide as substrate preincubated for 15 mins followed by substrate addition by fluorescence based microplate reader analysis	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2021) 220: 113453-113453 [PMID:33957387]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Ac-Leu-GlyLys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by fluorescence analysis	B	7.77	pIC50	17	nM	IC50	Bioorg Med Chem (2017) 25: 4123-4132 [PMID:28629630]
ChEMBL	Inhibition of human recombinant full-length HDAC2 using fluorophore conjugated substrate by fluorescence assay	B	7.8	pIC50	16	nM	IC50	J Med Chem (2011) 54: 5576-5582 [PMID:21721525]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2022) 72: 128846-128846 [PMID:35697181]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2022) 64: 128663-128663 [PMID:35272009]
ChEMBL	Inhibition of human recombinant HDAC2 using Ac-Leu-Gly-Lys(Ac)-AMC as substrate by measuring fluorescence intensity incubated for 30 mins by microplate reader assay	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2021) 217: 113363-113363 [PMID:33744687]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2021) 213: 113173-113173 [PMID:33493830]
ChEMBL	Inhibition of C-terminal 6XHis-tagged human recombinant full length HDAC2 (1 to 488 residues) expressed in baculovirus-infected Sf9 cells assessed as reduction in 7-amino-4-methylcoumarin release measured every 5 mins by fluorescence based analysis	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem (2021) 42: 116251-116251 [PMID:34116381]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2021) 224: 113672-113672 [PMID:34237620]
ChEMBL	Inhibition of human HDAC2 incubated for 30 mins by SpectraMax M2 microplate reader analysis	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic peptide RHKKAc-AMC as substrate by fluorescence-based assay	B	7.93	pIC50	11.7	nM	IC50	Bioorg Med Chem Lett (2023) 81: 129148-129148 [PMID:36690041]
ChEMBL	Inhibition of HDAC2 (unknown origin) using Fluor de lys as substrate by fluorescence based analysis	B	7.93	pIC50	11.7	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of HDAC2 (unknown origin) assessed as fluorescence intensity measured after 60 mins incubation at room temperature by trypsin-free microfluidic lab-on-a-chip assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2013) 56: 1772-1776 [PMID:23368884]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.96	pIC50	11	nM	IC50	J Med Chem (2022) 65: 16541-16569 [PMID:36449947]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorogenic substrate incubated for 30 mins by fluorescence based assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2022) 65: 3667-3683 [PMID:35152694]
ChEMBL	Inhibition of human recombinant HDAC2 using acetyllysine tripeptide coupled with 7-amino-4-methylcoumarin as substrate by fluorescence assay	B	7.99	pIC50	10.2	nM	IC50	Bioorg Med Chem Lett (2016) 26: 1265-1271 [PMID:26804233]
ChEMBL	Inhibition of HDAC2 (unknown origin)-mediated deacetylation preincubated for 5 mins prior to substrate addition measured after 30 mins by fluorescence assay	B	8	pIC50	10	nM	IC50	J Med Chem (2013) 56: 7997-8007 [PMID:24074025]
ChEMBL	Inhibition of recombinant human HDAC2	B	8.7	pIC50	2	nM	IC50	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of human recombinant HDAC2 after 30 mins by fluorometric assay	B	8.77	pIC50	1.7	nM	IC50	J Med Chem (2012) 55: 3777-3791 [PMID:22439863]
histone deacetylase 3/Histone deacetylase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379]
ChEMBL	Competitive inhibition of HDAC3 using KI-104 as substrate by fluorescence assay	B	7.44	pKi	36	nM	Ki	J Med Chem (2011) 54: 4694-4720 [PMID:21634430]
ChEMBL	Inhibition of HDAC3	B	7.54	pKi	29	nM	Ki