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Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)

Help on GtoMPdb Parasite Lifecycle Data

The GtoMPdb contains data detailing the parasitic lifecycle activity for antimalarial ligands and their target interactions. Top-level Plasmodium lifecycle stages (collective categories for one or more developmental forms of the parasite) have been defined by GtoMPdb against which interactions in the database have been annotated.

Each individual Parasite Lifecycle Stage page provides a detailed description of the stage and a table displaying all interactions (contained in the database) associated with it.

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Stage ID:3
Name:Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Associated with:33 targets
116 ligands
Description
The collective lifecycle stage that occurs as a result of asexual replication in erythrocytes of the host organism and can include:
  • erythrocytic merozoite, non-motile but recognises specific proteins on the surface of the erythrocyte and has an apical complex that facilitates entry into the host cell. This form of the parasite is also found in host hepatocytes (see hepatic merozoites).
  • trophozoite, the intracellular trophic form that develops from the merozoite. The young trophozoite has a distinctive 'ring' morphology in Geimsa-stained blood smears but this disappears as the parasite grows in size. During the trophic period the parasite ingests the host cell cytoplasm and breaks down the haemoglobin, producing non-toxic hemozoin as a by-product. After feeding and growth is complete the trophozoite undergoes asexual reproduction (schizony) and develops into a schizont.
  • erythrocytic schizont, a multinucleate form of the parasite that develops in erythrocytes from the trophozoite by schizogony with incomplete cytokineses. This developmental form is also found in host hepatocytes (see hepatic schizont).
Completion of cytokinesis produces several thousand merozoites from a single schizont, leading to rupture of the infected erythrocyte and release of merozoites into the bloodstream. These merozoites invade new erythrocytes and initiate either another cycle of schizogony or, under certain conditions, a small percentage of merozoites commit to sexual reproduction instead (see Plasmodium sexual blood stage).
It is the repeated cycles of schizogony in erythrocytes that leads to clinical symptoms: the simultaneous rupture of infected erythrocytes and the associated release of antigens and waste products accounts for the intermittent bouts of fever associated with malaria. As a result, almost all available antimalarial therapies target the asexual blood stage.

Interactions

Interactions
Key to terms and symbols Click column headers to sort
Target Ligand Sp. Action Value Parameter Reference
Plasmodium falciparum dihydroorotate dehydrogenase DSM265 PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 7.5 pIC50 68
pIC50 7.5 (IC50 3x10-8 M) [3H]-hypoxanthine incorporation [68]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum dihydroorotate dehydrogenase DSM421 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 7.4 – 7.8 pEC50 69
pEC50 7.8 (EC50 1.4x10-8 M) SYBR Green incorporation (72h assay) [69]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
pEC50 7.4 (EC50 4.3x10-8 M) SYBR Green incorporation (48h assay) [69]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum dihydroorotate dehydrogenase DSM421 Pv

Plasmodium vivax

P. vivax (Pv) is one of five protozoan parasite species of the genus Plasmodium that cause malaria in humans. Pv is the second most common cause of malaria in humans, after P. falciparum, and is prevalent in Southeast Asia and Latin America. Pv has a dormant liver stage that can reactivate and lead to clinical symptoms.
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 - 6.7 pEC50 69
pEC50 6.7 (EC50 1.8x10-7 M) [69]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Blood-stage: schizont maturation assay using P. vivax field isolates.
Plasmodium falciparum dihydroorotate dehydrogenase DSM502 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 7.8 pEC50 41
pEC50 7.8 (EC50 1.4x10-8 M) SYBR Green I [41]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum dihydroorotate dehydrogenase DSM502 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
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 - 7.8 pEC50 41
pEC50 7.8 (EC50 1.6x10-8 M) SYBR Green I [41]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum dihydroorotate dehydrogenase BRD9185 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
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 - 7.8 pEC50 50
pEC50 7.8 (EC50 1.6x10-8 M) SYBR Green I assay [50]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum dihydroorotate dehydrogenase DSM705 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 7.9 pEC50 63
pEC50 7.9 (EC50 1.2x10-8 M) SYBR Green I assay (72h) [63]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phenylalanyl-tRNA synthetase alpha subunit BRD3444 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
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 - 8.1 pEC50 39
pEC50 8.1 (EC50 9x10-9 M) SYBR Green I assay [39]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phenylalanyl-tRNA synthetase alpha subunit BRD7929 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
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 - 8.1 pEC50 39
pEC50 8.1 (EC50 9x10-9 M) SYBR Green I assay [39]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase compound 34c [PMID: 24641010] Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 6.5 pEC50 72
pEC50 6.5 (EC50 3.02x10-7 M) [3H]-hypoxanthine incorporation [72]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase compound 34c [PMID: 24641010] PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 6.6 pEC50 72
pEC50 6.6 (EC50 2.32x10-7 M) [3H]-hypoxanthine incorporation [72]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase compound 34c [PMID: 24641010] PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
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 - 6.5 pEC50 72
pEC50 6.5 (EC50 3.39x10-7 M) [3H]-hypoxanthine incorporation [72]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase compound 34c [PMID: 24641010] PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
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 - 6.0 pEC50 72
pEC50 6.0 (EC50 9.16x10-7 M) [3H]-hypoxanthine incorporation [72]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase DDD85646 Pf

Plasmodium falciparum

P. falciparum (Pf) is one of five protozoan parasite species of the genus Plasmodium that cause malaria in humans. Pf is responsible for the majority of malaria related deaths and is the most prevalent species in sub-Saharan Africa.
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 - 6.6 pEC50 92
pEC50 6.6 (EC50 2.5x10-7 M) SYBR Green assay [92]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase IMP-1002 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 7.5 pEC50 78
pEC50 7.5 (EC50 3.4x10-8 M) SYBR Green incorporation [78]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum N-myristoyltransferase IMP-1002 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
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 - 7.7 pEC50 78
pEC50 7.7 (EC50 2.04x10-8 M) SYBR Green incorporation [78]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 9.1 pIC50 74
pIC50 9.1 (IC50 7x10-10 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 9.3 pIC50 74
pIC50 9.3 (IC50 5x10-10 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
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 - 8.9 pIC50 74
pIC50 8.9 (IC50 1.4x10-9 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
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 - 9.2 pIC50 74
pIC50 9.2 (IC50 6x10-10 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin PfD6

Plasmodium falciparum D6

P. falciparum strain D6 (PfD6) was collected in Sierra Leone.
PfD6 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is generally considered drug sensitive with minor mefloquine resistance.
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 - 9.0 pIC50 74
pIC50 9.0 (IC50 1x10-9 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin Pf7G8

Plasmodium falciparum 7G8

P. falciparum strain 7G8 (Pf7G8) was subcloned from the IMTM22 strain by limiting dilution. The original IMTM22 strain was isolated from a 12 year old male near Manaus, Brazil in 1980.
Pf7G8 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). This strain is a gametocyte producer and is reported to be chloroquine-sensitive and pyrimethamine-resistant.
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 - 8.9 pIC50 74
pIC50 8.9 (IC50 1.2x10-9 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase cipargamin PfTM90C2A

Plasmodium falciparum TM90C2A

P. falciparum TM90C2A (PfTM90C2A) is a clinical isolate, first described during a Phase 2 clinical trial to determine atovaquone efficacy in Thailand (PMID:8651372). PfTM90C2A is sensitive to atovaquone.
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 - 9.3 pIC50 74
pIC50 9.3 (IC50 5x10-10 M) [3H]-hypoxanthine incorporation assay [74]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase (+)-SJ733 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
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 - 7.5 pEC50 35
pEC50 7.5 (EC50 3x10-8 M) SYBR Green I assay [35]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase (+)-SJ733 PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
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 - 7.2 pEC50 35
pEC50 7.2 (EC50 6x10-8 M) SYBR Green I assay [35]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase (+)-SJ733 PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
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 - 7.4 pEC50 35
pEC50 7.4 (EC50 4x10-8 M) SYBR Green I assay [35]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase (+)-SJ733 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 7.7 pEC50 35
pEC50 7.7 (EC50 2x10-8 M) SYBR Green I assay [35]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.7 pEC50 84
pEC50 7.7 (EC50 1.8x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
✖
 - 7.4 pEC50 84
pEC50 7.4 (EC50 3.8x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 PfTM90C2A

Plasmodium falciparum TM90C2A

P. falciparum TM90C2A (PfTM90C2A) is a clinical isolate, first described during a Phase 2 clinical trial to determine atovaquone efficacy in Thailand (PMID:8651372). PfTM90C2A is sensitive to atovaquone.
✖
 - 7.8 pEC50 84
pEC50 7.8 (EC50 1.4x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 7.7 pEC50 84
pEC50 7.7 (EC50 2.2x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 PfHB3

Plasmodium falciparum HB3

P. falciparum strain HB3 (PfHB3) was cloned from the Honduras I/CDC strain, originally isolated from a patient in Choluteca, Honduras, during an outbreak of urban malaria in January 1980.
PfHB3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be chloroquine sensitive.
✖
 - 7.5 pEC50 84
pEC50 7.5 (EC50 3.2x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 PfFCR3

Plasmodium falciparum FCR3

P. falciparum strain FCR3 (PfFCR3) was originally isolated from a patient in The Gambia, West Africa in 1976.
PfFCR3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). Differences in sensitivity of PfFCR3 clones to chloroquine have been reported, with the original isolate that was chloroquine sensitive rapidly acquiring resistance in culture (PMID: 7031656, PMID: 51801).
✖
 - 7.7 pEC50 84
pEC50 7.7 (EC50 1.9x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase PA21A092 PfTM90C2B

Plasmodium falciparum TM90C2B

P. falciparum TM90C2B (PfTM90C2B) is a clinical isolate, first described during a Phase 2 clinical trial to determine atovaquone efficacy in Thailand (PMID:8651372). A point mutation (Y268S) at the Qo site of cytochrome b results in a 3,000-fold loss of atovaquone sensitivity. It is used as a screening tool for the development of new cytochrome b inhibitors.
✖
 - 7.9 pEC50 84
pEC50 7.9 (EC50 1.3x10-8 M) [3H]-hypoxanthine incorporation [84]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase GSK030 Pf

Plasmodium falciparum

P. falciparum (Pf) is one of five protozoan parasite species of the genus Plasmodium that cause malaria in humans. Pf is responsible for the majority of malaria related deaths and is the most prevalent species in sub-Saharan Africa.
✖
 - 7.3 pIC50 15
pIC50 7.3 (IC50 5.2x10-8 M) [3H]-hypoxanthine incorporation [15]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase MMV1803022 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 6.3 pIC50 82
pIC50 6.3 (IC50 4.6x10-7 M) SYBR Green I assay [82]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase MMV897698 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 6.4 pIC50 83
pIC50 6.4 (IC50 3.8x10-7 M) SYBR Green I assay [83]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum non-SERCA-type Ca2+ -transporting P-ATPase compound 1a [WO2021204952A1] Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.7 pEC50 22
pEC50 7.7 (EC50 1.9x10-8 M) SYBR Green assay [22]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta MMV048 PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.6 pIC50 64
pIC50 7.6 (IC50 2.8x10-8 M) [3H]-hypoxanthine incorporation assay [64]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta compound 55 [PMID: 29889526] PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.2 pIC50 38
pIC50 7.2 (IC50 6.3x10-8 M) Parasite lactate dehydrogenase (pLDH ) assay [38]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta compound 55 [PMID: 29889526] PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 7.0 pIC50 38
pIC50 7.0 (IC50 1.02x10-7 M) Parasite lactate dehydrogenase (pLDH ) assay [38]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta MMV024101 PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 6.3 pIC50 38
pIC50 6.3 (IC50 5.43x10-7 M) Parasite lactate dehydrogenase (pLDH ) assay [38]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 8.3 pIC50 6
pIC50 8.3 (IC50 5.4x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
✖
 - 8.4 pIC50 6
pIC50 8.4 (IC50 4.2x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 8.3 pIC50 6
pIC50 8.3 (IC50 4.7x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 PfD6

Plasmodium falciparum D6

P. falciparum strain D6 (PfD6) was collected in Sierra Leone.
PfD6 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is generally considered drug sensitive with minor mefloquine resistance.
✖
 - 8.3 pIC50 6
pIC50 8.3 (IC50 5.2x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 Pf7G8

Plasmodium falciparum 7G8

P. falciparum strain 7G8 (Pf7G8) was subcloned from the IMTM22 strain by limiting dilution. The original IMTM22 strain was isolated from a 12 year old male near Manaus, Brazil in 1980.
Pf7G8 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). This strain is a gametocyte producer and is reported to be chloroquine-sensitive and pyrimethamine-resistant.
✖
 - 8.4 pIC50 6
pIC50 8.4 (IC50 4.1x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 8.2 pIC50 6
pIC50 8.2 (IC50 5.7x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta UCT943 PfHB3

Plasmodium falciparum HB3

P. falciparum strain HB3 (PfHB3) was cloned from the Honduras I/CDC strain, originally isolated from a patient in Choluteca, Honduras, during an outbreak of urban malaria in January 1980.
PfHB3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be chloroquine sensitive.
✖
 - 8.4 pIC50 6
pIC50 8.4 (IC50 3.7x10-9 M) [3H]-hypoxanthine incorporation [6]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta CHMFL-PI4K-127 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.6 pEC50 47
pEC50 7.6 (EC50 2.51x10-8 M) SYBR Green I assay [47]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta CHMFL-PI4K-127 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 7.5 pEC50 47
pEC50 7.5 (EC50 3.03x10-8 M) SYBR Green I assay [47]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta NCATS-SM3710 PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 9.4 pEC50 42
pEC50 9.4 (EC50 3.8x10-10 M) Viability measured using MitoProbe DiIC1(5) staining and flow cytometry [42]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum phosphatidylinositol 4-kinase beta KDU691 Pf

Plasmodium falciparum

P. falciparum (Pf) is one of five protozoan parasite species of the genus Plasmodium that cause malaria in humans. Pf is responsible for the majority of malaria related deaths and is the most prevalent species in sub-Saharan Africa.
✖
 - 7.5 pIC50 51
pIC50 7.5 (IC50 2.99x10-8 M) SYBR Green assay [51]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum elongation factor 2 M5717 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 9.0 pEC50 4
pEC50 9.0 (EC50 1x10-9 M) [3H]-hypoxanthine incorporation assay [4]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase fosmidomycin PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 6.5 pIC50 36
pIC50 6.5 (IC50 2.9x10-7 M) [3H]-hypoxanthine incorporation assay [36]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase fosmidomycin PfHB3

Plasmodium falciparum HB3

P. falciparum strain HB3 (PfHB3) was cloned from the Honduras I/CDC strain, originally isolated from a patient in Choluteca, Honduras, during an outbreak of urban malaria in January 1980.
PfHB3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be chloroquine sensitive.
✖
 - 6.5 pIC50 36
pIC50 6.5 (IC50 3.5x10-7 M) [3H]-hypoxanthine incorporation assay [36]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase fosmidomycin PfA2

Plasmodium falciparum A2

P. falciparum A2 (PfA2) originates from The Gambia (PMID:10477522).
✖
 - 6.4 pIC50 36
pIC50 6.4 (IC50 3.7x10-7 M) [3H]-hypoxanthine incorporation assay [36]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.8 pIC50 14
pIC50 7.8 (IC50 1.7x10-8 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
✖
 - <4.0 – 5.0 pIC50 14,96
pIC50 <5.0 (IC50 >1x10-5 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
pIC50 <4.0 (IC50 >1x10-4 M) [3H]-hypoxanthine incorporation assay using quadruple mutant P. falciparum (V1/S) [96]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - <5.0 pIC50 14
pIC50 <5.0 (IC50 >1x10-5 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfD6

Plasmodium falciparum D6

P. falciparum strain D6 (PfD6) was collected in Sierra Leone.
PfD6 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is generally considered drug sensitive with minor mefloquine resistance.
✖
 - 8.3 pIC50 14
pIC50 8.3 (IC50 4.6x10-9 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine Pf7G8

Plasmodium falciparum 7G8

P. falciparum strain 7G8 (Pf7G8) was subcloned from the IMTM22 strain by limiting dilution. The original IMTM22 strain was isolated from a 12 year old male near Manaus, Brazil in 1980.
Pf7G8 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). This strain is a gametocyte producer and is reported to be chloroquine-sensitive and pyrimethamine-resistant.
✖
 - 5.2 pIC50 14
pIC50 5.2 (IC50 6.689x10-6 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfW2

Plasmodium falciparum W2

P. falciparum strain W2 (PfW2) was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfW2 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and susceptible to mefloquine.
✖
 - <5.0 pIC50 14
pIC50 <5.0 (IC50 >1x10-5 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfTM90C2A

Plasmodium falciparum TM90C2A

P. falciparum TM90C2A (PfTM90C2A) is a clinical isolate, first described during a Phase 2 clinical trial to determine atovaquone efficacy in Thailand (PMID:8651372). PfTM90C2A is sensitive to atovaquone.
✖
 - 5.1 pIC50 14
pIC50 5.1 (IC50 8.231x10-6 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase pyrimethamine PfTM4

Plasmodium falciparum TM4

P. falciparum strain TM4 (PfTM4) was isolated from a patient at the Tropical Medical Hospital, Bangkok, Thailand in 1985.
✖
 - 7.2 pIC50 96
pIC50 7.2 (IC50 5.8x10-8 M) [3H]-hypoxanthine incorporation assay using wild-type P. falciparum (TM4) [96]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase P218 PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
✖
 - 7.3 pIC50 96
pIC50 7.3 (IC50 5.6x10-8 M) [3H]-hypoxanthine incorporation [96]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase P218 PfTM4

Plasmodium falciparum TM4

P. falciparum strain TM4 (PfTM4) was isolated from a patient at the Tropical Medical Hospital, Bangkok, Thailand in 1985.
✖
 - 8.3 pIC50 96
pIC50 8.3 (IC50 4.6x10-9 M) [3H]-hypoxanthine incorporation [96]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 8.4 pIC50 14
pIC50 8.4 (IC50 4.5x10-9 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil PfV1/S

Plasmodium falciparum V1/S

P. falciparum strain V1/S (PfV1/S) is an in vitro culture-adapted clone of V1, that originated in Vietnam.
PfV1/S can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and quinine.
✖
 - <5.0 pIC50 14
pIC50 <5.0 (IC50 >1x10-5 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 6.3 pIC50 14
pIC50 6.3 (IC50 4.45x10-7 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil PfD6

Plasmodium falciparum D6

P. falciparum strain D6 (PfD6) was collected in Sierra Leone.
PfD6 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is generally considered drug sensitive with minor mefloquine resistance.
✖
 - 8.8 pIC50 14
pIC50 8.8 (IC50 1.7x10-9 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil Pf7G8

Plasmodium falciparum 7G8

P. falciparum strain 7G8 (Pf7G8) was subcloned from the IMTM22 strain by limiting dilution. The original IMTM22 strain was isolated from a 12 year old male near Manaus, Brazil in 1980.
Pf7G8 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). This strain is a gametocyte producer and is reported to be chloroquine-sensitive and pyrimethamine-resistant.
✖
 - 6.4 pIC50 14
pIC50 6.4 (IC50 3.657x10-7 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil PfW2

Plasmodium falciparum W2

P. falciparum strain W2 (PfW2) was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfW2 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and susceptible to mefloquine.
✖
 - 6.0 pIC50 14
pIC50 6.0 (IC50 1.002x10-6 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase cycloguanil PfTM90C2A

Plasmodium falciparum TM90C2A

P. falciparum TM90C2A (PfTM90C2A) is a clinical isolate, first described during a Phase 2 clinical trial to determine atovaquone efficacy in Thailand (PMID:8651372). PfTM90C2A is sensitive to atovaquone.
✖
 - 5.4 pIC50 14
pIC50 5.4 (IC50 4.262x10-6 M) [3H]-hypoxanthine incorporation [14]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum cGMP-dependent protein kinase ML10 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 8.7 pEC50 2
pEC50 8.7 (EC50 2.1x10-9 M) [3H]-hypoxanthine incorporation [2]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum cGMP-dependent protein kinase MMV030084 Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.0 pIC50 85
pIC50 7.0 (IC50 1.09x10-7 M) 72 hour growth inhibition assay [85]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum cGMP-dependent protein kinase MMV030084 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 6.9 pIC50 85
pIC50 6.9 (IC50 1.2x10-7 M) 72 hour growth inhibition assay [85]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase halofuginone Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 9.1 pEC50 24
pEC50 9.1 (EC50 8.6x10-10 M) SYBR Green assay [24]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase halofuginone PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 9.1 pEC50 24
pEC50 9.1 (EC50 8.1x10-10 M) SYBR Green assay [24]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase compound 1 [PMID: 33929818] Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 8.0 pIC50 60
pIC50 8.0 (IC50 1x10-8 M) DAPI incorporation assay (72h) [60]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase compound 1 [PMID: 33929818] PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.7 pIC50 60
pIC50 7.7 (IC50 1.8x10-8 M) [3H]-hypoxanthine incorporation assay (72h) [60]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase compound 1 [PMID: 33929818] PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 7.7 pIC50 60
pIC50 7.7 (IC50 2x10-8 M) [3H]-hypoxanthine incorporation assay (72h) [60]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase compound 1 [PMID: 33929818] Pf7G8

Plasmodium falciparum 7G8

P. falciparum strain 7G8 (Pf7G8) was subcloned from the IMTM22 strain by limiting dilution. The original IMTM22 strain was isolated from a 12 year old male near Manaus, Brazil in 1980.
Pf7G8 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). This strain is a gametocyte producer and is reported to be chloroquine-sensitive and pyrimethamine-resistant.
✖
 - 7.7 pIC50 60
pIC50 7.7 (IC50 1.8x10-8 M) [3H]-hypoxanthine incorporation assay (72h) [60]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase compound 1 [PMID: 33929818] PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 7.6 pIC50 60
pIC50 7.6 (IC50 2.4x10-8 M) [3H]-hypoxanthine incorporation assay (72h) [60]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum prolyl-tRNA synthetase compound 1 [PMID: 33929818] PfTM90C2B

Plasmodium falciparum TM90C2B

P. falciparum TM90C2B (PfTM90C2B) is a clinical isolate, first described during a Phase 2 clinical trial to determine atovaquone efficacy in Thailand (PMID:8651372). A point mutation (Y268S) at the Qo site of cytochrome b results in a 3,000-fold loss of atovaquone sensitivity. It is used as a screening tool for the development of new cytochrome b inhibitors.
✖
 - 7.8 pIC50 60
pIC50 7.8 (IC50 1.7x10-8 M) [3H]-hypoxanthine incorporation assay (72h) [60]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum threonyl-tRNA synthetase borrelidin Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 8.9 – 9.0 pIC50 56
pIC50 9.0 (IC50 9.7x10-10 M) Parasite lactate dehydrogenase (pLDH ) assay following 96 hours in culture [56]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
pIC50 8.9 (IC50 1.24x10-9 M) Parasite lactate dehydrogenase (pLDH ) assay following 48 hours in culture [56]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum threonyl-tRNA synthetase borrelidin PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 8.7 pIC50 62
pIC50 8.7 (IC50 1.9x10-9 M) Parasite lactate dehydrogenase (pLDH ) assay. [62]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum threonyl-tRNA synthetase borrelidin PfFCR3

Plasmodium falciparum FCR3

P. falciparum strain FCR3 (PfFCR3) was originally isolated from a patient in The Gambia, West Africa in 1976.
PfFCR3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). Differences in sensitivity of PfFCR3 clones to chloroquine have been reported, with the original isolate that was chloroquine sensitive rapidly acquiring resistance in culture (PMID: 7031656, PMID: 51801).
✖
 - 8.7 pIC50 62
pIC50 8.7 (IC50 1.8x10-9 M) Parasite lactate dehydrogenase (pLDH ) assay [62]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase cladosporin Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.3 pIC50 26
pIC50 7.3 (IC50 4.54x10-8 M) SYBR Green assay [26]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase cladosporin PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.1 pIC50 26
pIC50 7.1 (IC50 8.79x10-8 M) SYBR Green assay [26]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase cladosporin PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 7.1 pIC50 26
pIC50 7.1 (IC50 8.01x10-8 M) SYBR Green assay [26]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase cladosporin PfD6

Plasmodium falciparum D6

P. falciparum strain D6 (PfD6) was collected in Sierra Leone.
PfD6 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is generally considered drug sensitive with minor mefloquine resistance.
✖
 - 7.1 pIC50 26
pIC50 7.1 (IC50 7.21x10-8 M) SYBR Green assay [26]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase compound 5 [PMID: 30894487] Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 6.6 pEC50 3
pEC50 6.6 (EC50 2.7x10-7 M) SYBRgreen I [3]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase compound 5 [PMID: 30894487] PfNF54

Plasmodium falciparum NF54

P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands.
PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 6.4 pEC50 3
pEC50 6.4 (EC50 3.9x10-7 M) [3H]-hypoxanthine incorporation [3]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum lysyl-tRNA synthetase compound 5 [PMID: 30894487] PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 6.3 pEC50 3
pEC50 6.3 (EC50 5.1x10-7 M) [3H]-hypoxanthine incorporation [3]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase MMV008138 PfW2

Plasmodium falciparum W2

P. falciparum strain W2 (PfW2) was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfW2 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine and susceptible to mefloquine.
✖
 - 7.0 pEC50 93
pEC50 7.0 (EC50 1.1x10-7 M) [93]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase MMV008138 PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 6.6 pIC50 94
pIC50 6.6 (IC50 2.5x10-7 M) SYBR green assay [94]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase sulfadoxine Pf3D7

Plasmodium falciparum 3D7

P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1).
Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine.
✖
 - 7.1 pIC50 89
pIC50 7.1 (IC50 7.3x10-8 M) [3H]-hypoxanthine incorporation [89]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase sulfadoxine PfK1

Plasmodium falciparum K1

P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979.
PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain.
✖
 - 6.7 pIC50 89
pIC50 6.7 (IC50 2.23x10-7 M) [3H]-hypoxanthine incorporation [89]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase sulfadoxine PfDd2

Plasmodium falciparum Dd2

P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy.
PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine.
✖
 - 5.4 pIC50 89
pIC50 5.4 (IC50 3.97x10-6 M) [3H]-hypoxanthine incorporation [89]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase sulfadoxine PfHB3

Plasmodium falciparum HB3

P. falciparum strain HB3 (PfHB3) was cloned from the Honduras I/CDC strain, originally isolated from a patient in Choluteca, Honduras, during an outbreak of urban malaria in January 1980.
PfHB3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be chloroquine sensitive.
✖
 - 8.4 pIC50 89
pIC50 8.4 (IC50 4x10-9 M) [3H]-hypoxanthine incorporation [89]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Description: Parasite growth inhibition assay
Plasmodium falciparum hexose transporter