ChEMBL ligand: CHEMBL193240 (B-9302-107, B9302-107, BY217, BY-217, BYK20869, BYK-20869, Daliresp, Daxas, Roflumilast)
Class 1 phosphodiesterase PDEB1 in Trypanosoma brucei [ChEMBL: CHEMBL2010636] [UniProtKB: Q8WQX9] There should be some charts here, you may need to enable JavaScript!
phosphodiesterase 3A/Phosphodiesterase 3A in Human [ChEMBL: CHEMBL241] [GtoPdb: 1298] [UniProtKB: Q14432] There should be some charts here, you may need to enable JavaScript!
phosphodiesterase 4A/Phosphodiesterase 4A in Human [ChEMBL: CHEMBL254] [GtoPdb: 1300] [UniProtKB: P27815] There should be some charts here, you may need to enable JavaScript!
phosphodiesterase 4B/Phosphodiesterase 4B in Human [ChEMBL: CHEMBL275] [GtoPdb: 1301] [UniProtKB: Q07343] There should be some charts here, you may need to enable JavaScript!
phosphodiesterase 4C/Phosphodiesterase 4C in Human [ChEMBL: CHEMBL291] [GtoPdb: 1302] [UniProtKB: Q08493] There should be some charts here, you may need to enable JavaScript!
phosphodiesterase 4D/Phosphodiesterase 4D in Human [ChEMBL: CHEMBL288] [GtoPdb: 1303] [UniProtKB: Q08499] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Class 1 phosphodiesterase PDEB1 in Trypanosoma brucei (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2010636] [UniProtKB: Q8WQX9]
ChEMBL	Inhibition of full length Trypanosoma brucei PDEB1 expressed in Sf21 insect cells using cAMP as substrate by scintillation proximity assay	B	5.5	pKi	3162.28	nM	Ki	J Med Chem (2018) 61: 3870-3888 [PMID:29672041]
phosphodiesterase 3A/Phosphodiesterase 3A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL241] [GtoPdb: 1298] [UniProtKB: Q14432]
ChEMBL	Inhibition of core catalytic domains PDE3A (unknown origin)	B	4	pIC50	>100000	nM	IC50	Bioorg. Med. Chem. Lett. (2013) 23: 5311-5316 [PMID:23988356]
phosphodiesterase 4A/Phosphodiesterase 4A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL254] [GtoPdb: 1300] [UniProtKB: P27815]
ChEMBL	Inhibition of human PDE4A4 assessed as inhibition of [3H]cAMP hydrolysis to [3H]AMP after 15 mins by scintillation proximity assay	B	9.23	pIC50	0.59	nM	IC50	Eur. J. Med. Chem. (2011) 46: 4946-4956 [PMID:21871695]
ChEMBL	Inhibition of human full length PDE4A4 expressed in baculovirus infected sf21 cells	B	9.46	pIC50	0.35	nM	IC50	Bioorg. Med. Chem. Lett. (2013) 23: 4308-4314 [PMID:23806553]
ChEMBL	Inhibition of human recombinant PDE4A using [3H]cAMP as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 15 mins by SPA bead based scintillation counting analysis	B	9.68	pIC50	0.21	nM	IC50	Eur J Med Chem (2020) 204: 112492-112492 [PMID:32717478]
phosphodiesterase 4B/Phosphodiesterase 4B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL275] [GtoPdb: 1301] [UniProtKB: Q07343]
ChEMBL	Inhibition of human PDE4B2 catalytic domain and UCR2 (92 to 521) expressed in Escherichia coli BL21 using [3H]-cAMP/[3H]-cGMP after 15 mins by liquid scintillation counting	B	6.17	pIC50	670	nM	IC50	Bioorg. Med. Chem. Lett. (2012) 22: 3261-3264 [PMID:22483586]
ChEMBL	Inhibition of human His-tagged PDE4B catalytic domain expressed in Escherichia coli BL21-CodonPlus(DE3) cells using [3H]cAMP or [3H]cGMP as substrate incubated for 30 mins by scintillation counting method	B	7.08	pIC50	84	nM	IC50	J Med Chem (2016) 59: 7029-7065 [PMID:26908025]
ChEMBL	Inhibition of PDE4B (unknown origin)	B	8.89	pIC50	1.3	nM	IC50	Eur J Med Chem (2019) 174: 198-215 [PMID:31035240]
ChEMBL	Inhibition of core catalytic domains PDE4B (unknown origin)	B	8.96	pIC50	1.1	nM	IC50	Bioorg. Med. Chem. Lett. (2013) 23: 5311-5316 [PMID:23988356]
ChEMBL	Inhibition of human recombinant PDE4B2 assessed as inhibition of cAMP hydrolysis by colorimetric assay	B	9	pIC50	1	nM	IC50	Bioorg. Med. Chem. Lett. (2014) 24: 249-253 [PMID:24300734]
ChEMBL	Inhibition of human recombinant PDE4B2-mediated cAMP hydrolysis for 30 mins by colorimetric assay	B	9	pIC50	1	nM	IC50	Bioorg. Med. Chem. Lett. (2012) 22: 1523-1526 [PMID:22297114]
ChEMBL	Inhibition of human PDE4B by ELISA	B	9.05	pIC50	0.9	nM	IC50	Eur J Med Chem (2018) 148: 477-486 [PMID:29477888]
ChEMBL	Inhibition of human U937 cells-derived PDE4B using [3H]-cAMP as substrate after 30 mins	B	9.05	pIC50	0.89	nM	IC50	Bioorg Med Chem Lett (2018) 28: 1188-1193 [PMID:29545101]
ChEMBL	Inhibition of human PDE4B by scintillation counting method	B	9.08	pIC50	0.84	nM	IC50	J Med Chem (2020) 63: 10594-10617 [PMID:32255344]
ChEMBL	Inhibition of human PDE4B	B	9.08	pIC50	0.84	nM	IC50	Bioorg. Med. Chem. (2010) 18: 2204-2218 [PMID:20188577]
ChEMBL	Inhibition of the human PDE4B catalytic domain after 30 mins in presence of [3H]cAMP/[3H]cGMP by scintillation counting	B	9.08	pIC50	0.84	nM	IC50	J Med Chem (2016) 59: 8712-8756 [PMID:27299736]
ChEMBL	Inhibition of human PDE4B2	B	9.1	pIC50	0.8	nM	IC50	Bioorg. Med. Chem. Lett. (2014) 24: 249-253 [PMID:24300734]
ChEMBL	Inhibition of human recombinant PDE4B2 assessed as decrease in cAMP hydrolysis preincubated with substrate prior to enzyme addition measured after 30 mins by colorimetric assay method	B	9.1	pIC50	0.8	nM	IC50	Bioorg. Med. Chem. Lett. (2013) 23: 1548-1552 [PMID:23375225]
ChEMBL	Inhibition of recombinant human PDE4B1 using [3H]-cAMP as substrate preincubated for 15 mins followed substrate addition measured after 60 mins by scintillation proximity assay	B	9.15	pIC50	0.7	nM	IC50	J Med Chem (2018) 61: 2472-2489 [PMID:29502405]
ChEMBL	Inhibition of recombinant human PDE4B1 expressed in African green monkey COS7 cells using cAMP as substrate	B	9.15	pIC50	0.7	nM	IC50	J Med Chem (2018) 61: 2472-2489 [PMID:29502405]
ChEMBL	Inhibition of human PDE4B1 assessed as inhibition of [3H]cAMP hydrolysis to [3H]AMP after 15 mins by scintillation proximity assay	B	9.16	pIC50	0.69	nM	IC50	Eur. J. Med. Chem. (2011) 46: 4946-4956 [PMID:21871695]
ChEMBL	Inhibition of human recombinant PDE4B1 assessed as reduction in [3H]cAMP hydrolysis to [3H]AMP incubated for 60 mins by PDE-SPA assay	B	9.16	pIC50	0.69	nM	IC50	J Med Chem (2016) 59: 10479-10497 [PMID:27933955]
ChEMBL	Inhibition of human recombinant PDE4B (152 to 564 amino acids) using cAMP as substrate after 30 mins by plate reader analysis	B	9.31	pIC50	0.49	nM	IC50	Bioorg. Med. Chem. Lett. (2013) 23: 3325-3328 [PMID:23602400]
ChEMBL	Inhibition of recombinant human PDE4B using cAMP as substrate after 30 mins	B	9.31	pIC50	0.49	nM	IC50	Bioorg. Med. Chem. (2013) 21: 7025-7037 [PMID:24094436]
ChEMBL	Inhibition of human recombinant PDE4B1 activity assessed as residual cAMP concentration by HTRF assay	B	9.4	pIC50	0.4	nM	IC50	Bioorg. Med. Chem. Lett. (2014) 24: 4594-4597 [PMID:25149511]
GtoPdb	-	-	9.4	pIC50	-	-	-	Bioorg Med Chem Lett (2009) 19: 1380-5 [PMID:19195882]
ChEMBL	Inhibition of human recombinant PDE4B by scintillation proximity assay	B	9.4	pIC50	0.4	nM	IC50	Bioorg. Med. Chem. Lett. (2009) 19: 1380-1385 [PMID:19195882]
ChEMBL	Inhibition of human recombinant PDE4B by scintillation proximity assay	B	9.4	pIC50	0.4	nM	IC50	Bioorg. Med. Chem. Lett. (2009) 19: 5261-5265 [PMID:19656678]
ChEMBL	Inhibition of human PDE4B using [3H]cAMP as substrate incubated for 5 mins followed by substrate addition and measured after 10 mins by scintillation proximity assay	B	9.7	pIC50	0.2	nM	IC50	Bioorg Med Chem (2016) 24: 5702-5716 [PMID:27713015]
ChEMBL	Inhibition of human recombinant PDE4B using [3H]cAMP as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 15 mins by SPA bead based scintillation counting analysis	B	9.82	pIC50	0.15	nM	IC50	Eur J Med Chem (2020) 204: 112492-112492 [PMID:32717478]
phosphodiesterase 4C/Phosphodiesterase 4C in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL291] [GtoPdb: 1302] [UniProtKB: Q08493]
ChEMBL	Inhibition of human recombinant PDE4C using [3H]cAMP as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 15 mins by SPA bead based scintillation counting analysis	B	8.77	pIC50	1.7	nM	IC50	Eur J Med Chem (2020) 204: 112492-112492 [PMID:32717478]
phosphodiesterase 4D/Phosphodiesterase 4D in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL288] [GtoPdb: 1303] [UniProtKB: Q08499]
ChEMBL	Inhibition of human PDE4D2 (86 to 413 residues) catalytic domain using [3H]-cAMP as substrate after 15 mins by liquid scintillation counting method	B	6.32	pIC50	480	nM	IC50	Eur J Med Chem (2019) 163: 512-526 [PMID:30553143]
ChEMBL	Inhibition of human PDE4D2 catalytic domain (86 to 413) expressed in Escherichia coli BL21 using [3H]-cAMP/[3H]-cGMP after 15 mins by liquid scintillation counting	B	6.34	pIC50	460	nM	IC50	Bioorg. Med. Chem. Lett. (2012) 22: 3261-3264 [PMID:22483586]
ChEMBL	Inhibition of human U937 cells-derived PDE4D using [3H]-cAMP as substrate after 30 mins	B	8.96	pIC50	1.1	nM	IC50	Bioorg Med Chem Lett (2018) 28: 1188-1193 [PMID:29545101]
ChEMBL	Inhibition of human recombinant PDE4D2 activity assessed as residual cAMP concentration by HTRF assay	B	9.15	pIC50	0.7	nM	IC50	Bioorg. Med. Chem. Lett. (2014) 24: 4594-4597 [PMID:25149511]
ChEMBL	Inhibition of human PDE4D catalytic domain using [3H]-cAMP or [3H]-cGMP incubated for 30 mins by scintillation counting method	B	9.17	pIC50	0.68	nM	IC50	EP-2674161-A1. FMO3 inhibitors for treating pain (null)
ChEMBL	Inhibition of human PDE4D by scintillation counting method	B	9.17	pIC50	0.68	nM	IC50	J Med Chem (2020) 63: 10594-10617 [PMID:32255344]
ChEMBL	Inhibition of human His-tagged PDE4D catalytic domain expressed in Escherichia coli BL21-CodonPlus(DE3) cells using [3H]cAMP or [3H]cGMP as substrate incubated for 30 mins by scintillation counting method	B	9.17	pIC50	0.68	nM	IC50	J Med Chem (2016) 59: 7029-7065 [PMID:26908025]
ChEMBL	Inhibition of PDE4D2 (unknown origin)	B	9.4	pIC50	0.4	nM	IC50	J Med Chem (2020) 63: 3370-3380 [PMID:32115956]
ChEMBL	Inhibition of PDE4D (unknown origin) using cAMP as substrate	B	9.4	pIC50	0.4	nM	IC50	J Med Chem (2019) 62: 5579-5593 [PMID:31099559]
ChEMBL	Inhibition of human PDE4D3 assessed as inhibition of [3H]cAMP hydrolysis to [3H]AMP after 15 mins by scintillation proximity assay	B	9.57	pIC50	0.27	nM	IC50	Eur. J. Med. Chem. (2011) 46: 4946-4956 [PMID:21871695]
ChEMBL	Inhibition of PDE4D (unknown origin)	B	9.7	pIC50	0.2	nM	IC50	Eur J Med Chem (2019) 174: 198-215 [PMID:31035240]
ChEMBL	Inhibition of human recombinant PDE4D using [3H]cAMP as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 15 mins by SPA bead based scintillation counting analysis	B	9.89	pIC50	0.13	nM	IC50	Eur J Med Chem (2020) 204: 112492-112492 [PMID:32717478]

ChEMBL data shown on this page come from version 31:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]