furosemide [Ligand Id: 4839] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL35 (Aluzine 20, Aluzine 40, Aluzine 500, Aquamed, Disal, Diuresal, Dryptal, Froop, Frumax, Frumil, Frusemide, Frusetic, Frusid, Frusol, Furosemide, Furosemidum, Hydroled, Lasix, Lasix ret, LB-502, Logirene, Marsemide, Mirfat, NSC-269420, Oedemex, Rusyde, Tenkofruse)
  • hydroxysteroid 11-beta dehydrogenase 1/11-beta-hydroxysteroid dehydrogenase 1 in Human [ChEMBL: CHEMBL4235] [GtoPdb: 2763] [UniProtKB: P28845]
  • hydroxysteroid 11-beta dehydrogenase 1/11-beta-hydroxysteroid dehydrogenase 1 in Mouse [ChEMBL: CHEMBL3910] [GtoPdb: 2763] [UniProtKB: P50172]
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  • Sodium/bile acid and sulphated solute cotransporter 1/Bile acid transporter in Human [ChEMBL: CHEMBL5287] [GtoPdb: 959] [UniProtKB: Q14973]
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  • carbonic anhydrase 1/Carbonic anhydrase I in Human [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
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  • carbonic anhydrase 2/Carbonic anhydrase II in Human [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
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  • carbonic anhydrase 4/Carbonic anhydrase IV in Human [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748]
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  • carbonic anhydrase 9/Carbonic anhydrase IX in Human [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790]
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  • carbonic anhydrase 5A/Carbonic anhydrase VA in Human [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218]
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  • carbonic anhydrase 7/Carbonic anhydrase VII in Human [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166]
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  • carbonic anhydrase 12/Carbonic anhydrase XII in Human [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570]
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  • carbonic anhydrase 13/Carbonic anhydrase XIII in Mouse [ChEMBL: CHEMBL2186] [GtoPdb: 2748] [UniProtKB: Q9D6N1]
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  • carbonic anhydrase 14/Carbonic anhydrase XIV in Human [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7]
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  • CDGSH iron-sulfur domain-containing protein 1 in Human [ChEMBL: CHEMBL1795168] [UniProtKB: Q9NZ45]
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  • Holo-[acyl-carrieir-protein] synthase in Bacillus subtilis (strain 168) [ChEMBL: CHEMBL4734] [UniProtKB: P96618]
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  • Organic anion transporter 1/Solute carrier family 22 member 6 in Rat [ChEMBL: CHEMBL1777665] [GtoPdb: 1025] [UniProtKB: O35956]
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  • OATP2A1/Solute carrier organic anion transporter family member 2A1 in Rat [ChEMBL: CHEMBL2073680] [GtoPdb: 1223] [UniProtKB: Q00910]
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  • Kidney-specific Na-K-Cl symporter in Human [GtoPdb: 968] [UniProtKB: Q13621]
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  • Basolateral Na-K-Cl symporter in Human [GtoPdb: 969] [UniProtKB: P55011]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
hydroxysteroid 11-beta dehydrogenase 1/11-beta-hydroxysteroid dehydrogenase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4235] [GtoPdb: 2763] [UniProtKB: P28845]
ChEMBL Inhibition of human 11beta-HSD1 expressed in HEK293 cells assessed as conversion of [3H]cortisone to [3H]cortisol by scintillation proximity assay in presence of NADPH B 5.42 pIC50 3760 nM IC50 Eur J Med Chem (2009) 44: 1167-1171 [PMID:18653260]
hydroxysteroid 11-beta dehydrogenase 1/11-beta-hydroxysteroid dehydrogenase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3910] [GtoPdb: 2763] [UniProtKB: P50172]
ChEMBL Inhibition of mouse 11beta-HSD1 expressed in HEK293 cells assessed as conversion of [3H]cortisone to [3H]cortisol by scintillation proximity assay in presence of NADPH B 5.42 pIC50 3760 nM IC50 Eur J Med Chem (2009) 44: 1167-1171 [PMID:18653260]
Adenosine A1 receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2304404] [UniProtKB: P47745]
ChEMBL Binding affinity against adenosine A1 receptor in guinea pig forebrain membranes using N6-[3H]cyclohexyladenosine as radioligand B 4 pKi >100000 nM Ki J Med Chem (1992) 35: 3066-3075 [PMID:1501234]
Sodium/bile acid and sulphated solute cotransporter 1/Bile acid transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5287] [GtoPdb: 959] [UniProtKB: Q14973]
ChEMBL TP_TRANSPORTER: inhibition of Taurocholate uptake in NTCP-expressing HeLa cells F 4.82 pIC50 15000 nM IC50 J Pharmacol Exp Ther (1999) 291: 1204-1209 [PMID:10565843]
carbonic anhydrase 1/Carbonic anhydrase I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
ChEMBL Inhibition of human recombinant full length CA1 by stopped-flow CO2 hydration method B 7.21 pKi 62 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 2/Carbonic anhydrase II in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
ChEMBL Inhibition of human recombinant full length CA2 by stopped-flow CO2 hydration method B 7.19 pKi 65 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 4/Carbonic anhydrase IV in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748]
ChEMBL Inhibition of human recombinant full length CA4 by stopped-flow CO2 hydration method B 6.25 pKi 564 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 9/Carbonic anhydrase IX in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790]
ChEMBL Inhibition of human recombinant CA9 catalytic domain by stopped-flow CO2 hydration method B 6.38 pKi 420 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 5A/Carbonic anhydrase VA in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218]
ChEMBL Inhibition of human recombinant full length CA5A by stopped-flow CO2 hydration method B 6.3 pKi 499 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
Carbonic anhydrase VB in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3969] [UniProtKB: Q9Y2D0]
ChEMBL Inhibition of human recombinant full length CA5B by stopped-flow CO2 hydration method B 6.49 pKi 322 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
Carbonic anhydrase VI in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3025] [UniProtKB: P23280]
ChEMBL Inhibition of human recombinant full length CA6 by stopped-flow CO2 hydration method B 6.61 pKi 245 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 7/Carbonic anhydrase VII in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166]
ChEMBL Inhibition of human recombinant full length CA7 by stopped-flow CO2 hydration method B 6.29 pKi 513 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 12/Carbonic anhydrase XII in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570]
ChEMBL Inhibition of human recombinant CA12 catalytic domain by stopped-flow CO2 hydration method B 6.58 pKi 261 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 13/Carbonic anhydrase XIII in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2186] [GtoPdb: 2748] [UniProtKB: Q9D6N1]
ChEMBL Inhibition of mouse recombinant full length CA13 by stopped-flow CO2 hydration method B 6.26 pKi 550 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 14/Carbonic anhydrase XIV in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7]
ChEMBL Inhibition of human recombinant full length CA14 by stopped-flow CO2 hydration method B 7.28 pKi 52 nM Ki Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
CDGSH iron-sulfur domain-containing protein 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1795168] [UniProtKB: Q9NZ45]
ChEMBL Displacement of [3H]rosiglitazone from recombinant human C-terminal His-tagged MitoNEET cytosolic domain (32 to 108 residues) expressed in Escherichia coli BL21 by Cheng-Prusoff analysis B 5.64 pKi 2285 nM Ki Bioorg Med Chem Lett (2016) 26: 5350-5353 [PMID:27687671]
ChEMBL Displacement of [3H]rosiglitazone from recombinant human C-terminal His-tagged MitoNEET cytosolic domain (32 to 108 residues) expressed in Escherichia coli BL21 by scintillation proximity assay B 4.27 pIC50 53460 nM IC50 Bioorg Med Chem Lett (2016) 26: 5350-5353 [PMID:27687671]
GPR35/G-protein coupled receptor 35 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293267] [GtoPdb: 102] [UniProtKB: Q9HC97]
ChEMBL Displacement of [3H]PSB-13253 from human recombinant GPR35 exprssed in CHO cells by liquid scintillation counting analysis B 5.49 pKi 3270 nM Ki J Med Chem (2013) 56: 7084-7099 [PMID:23888932]
ChEMBL Agonist activity at C-terminal beta-galactosidase tagged human recombinant GPR35 expressed in CHO cells after 90 mins by beta-arrestin recruitment assay B 4.95 pEC50 11200 nM EC50 J Med Chem (2013) 56: 7084-7099 [PMID:23888932]
ChEMBL Agonist activity at human GPR35 by Ca+2 release assay B 5.21 pEC50 6200 nM EC50 J Med Chem (2013) 56: 7084-7099 [PMID:23888932]
GtoPdb - - 5.63 pEC50 - - - Pharmacology (2012) 89: 13-7 [PMID:22236570]
Holo-[acyl-carrieir-protein] synthase in Bacillus subtilis (strain 168) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4734] [UniProtKB: P96618]
ChEMBL Inhibitory activity against Bacillus subtilis AcpS by HTRF assay B 4.9 pIC50 12700 nM IC50 J Med Chem (2005) 48: 7960-7969 [PMID:16335920]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL Antiplasmodial activity against Plasmodium falciparum D10 after 72 hrs by SYBR green assay F 4.8 pIC50 15848.93 nM IC50 Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay F 4.9 pIC50 12589.25 nM IC50 Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay F 4.9 pIC50 12589.25 nM IC50 Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
Organic anion transporter 1/Solute carrier family 22 member 6 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1777665] [GtoPdb: 1025] [UniProtKB: O35956]
ChEMBL TP_TRANSPORTER: inhibition of PAH uptake in Xenopus laevis oocytes F 5.02 pKi 9500 nM Ki J Pharmacol Exp Ther (2000) 295: 261-265 [PMID:10991988]
OATP2A1/Solute carrier organic anion transporter family member 2A1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073680] [GtoPdb: 1223] [UniProtKB: Q00910]
ChEMBL TP_TRANSPORTER: inhibition of PGF2alpha uptake in PGT-expressing HeLa cells F 4.33 pKi 47000 nM Ki Science (1995) 268: 866-869 [PMID:7754369]
Kidney-specific Na-K-Cl symporter in Human [GtoPdb: 968] [UniProtKB: Q13621]
GtoPdb - - 5.15 pIC50 - - - Naunyn Schmiedebergs Arch Pharmacol (2002) 365: 193-9 [PMID:11882915]
Basolateral Na-K-Cl symporter in Human [GtoPdb: 969] [UniProtKB: P55011]
GtoPdb - - 5.13 pIC50 - - - Naunyn Schmiedebergs Arch Pharmacol (2002) 365: 193-9 [PMID:11882915]

ChEMBL data shown on this page come from version 32:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]