ChEMBL ligand: CHEMBL273807 (MRS-1754)
A1 receptor/Adenosine A1 receptor in Human [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542] A1 receptor/Adenosine A1 receptor in Mouse [ChEMBL: CHEMBL3688] [GtoPdb: 18] [UniProtKB: Q60612] A1 receptor/Adenosine A1 receptor in Rat [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099] There should be some charts here, you may need to enable JavaScript!
A2A receptor/Adenosine A2a receptor in Human [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274] A2A receptor/Adenosine A2a receptor in Mouse [ChEMBL: CHEMBL2115] [GtoPdb: 19] [UniProtKB: Q60613] A2A receptor/Adenosine A2a receptor in Rat [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543] There should be some charts here, you may need to enable JavaScript!
A2B receptor/Adenosine A2b receptor in Human [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275] A2B receptor/Adenosine A2b receptor in Mouse [ChEMBL: CHEMBL2237] [GtoPdb: 20] [UniProtKB: Q60614] A2B receptor/Adenosine A2b receptor in Rat [ChEMBL: CHEMBL2592] [GtoPdb: 20] [UniProtKB: P29276] There should be some charts here, you may need to enable JavaScript!
A3 receptor/Adenosine A3 receptor in Human [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8] A3 receptor/Adenosine A3 receptor in Mouse [ChEMBL: CHEMBL1075269] [GtoPdb: 21] [UniProtKB: Q61618] A3 receptor/Adenosine A3 receptor in Rat [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
GtoPdb	-	-	6.39	pKi	403	nM	Ki	J Med Chem (2000) 43: 1165-72 [PMID:10737749]
ChEMBL	Binding affinity to recombinant human adenosine A1 receptor	B	6.39	pKi	407.38	nM	Ki	J. Med. Chem. (2006) 49: 282-299 [PMID:16392813]
ChEMBL	Binding affinity towards human Adenosine A1 receptor	B	6.39	pKi	403	nM	Ki	J. Med. Chem. (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Antagonist activity against recombinant human adenosine A1 receptor expressed in HEK293 cells in presence of [125I]IABA radioligand.	F	6.39	pKi	403	nM	Ki	J. Med. Chem. (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Binding affinity to human adenosine A1 receptor	B	6.39	pKi	403	nM	Ki	J. Med. Chem. (2012) 55: 797-811 [PMID:22148859]
ChEMBL	Displacement of [125I]IABA from recombinant human A1 adenosine receptor expressed in HEK293 cell membranes measured after 3 hrs	B	6.39	pKi	403	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
ChEMBL	Binding affinity to human adenosine A1 receptor	B	6.39	pKi	403	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Antagonist activity against human adenosine A1 receptor	F	6.39	pKi	403	nM	Ki	Eur. J. Med. Chem. (2010) 45: 3459-3471 [PMID:20537438]
ChEMBL	Displacement of [3H]CPX from human adenosine A3 receptor expressed in CHO cells	B	6.4	pKi	400	nM	Ki	J. Med. Chem. (2006) 49: 3682-3692 [PMID:16759111]
ChEMBL	Binding affinity against Adenosine A1 receptor labeled by [3H]CPX in membranes prepared from Chinese hamster ovary cells	B	6.68	pKi	210	nM	Ki	Bioorg. Med. Chem. Lett. (2005) 15: 609-612 [PMID:15664822]
A1 receptor/Adenosine A1 receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3688] [GtoPdb: 18] [UniProtKB: Q60612]
ChEMBL	Displacement of [3H]CCPA from recombinant mouse A1 adenosine receptor expressed in CHO cell membranes measured after 60 mins by liquid scintillation counting method	B	8.84	pKi	1.45	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A1 receptor/Adenosine A1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099]
GtoPdb	-	-	7.77	pKi	16.8	nM	Ki	J Med Chem (2000) 43: 1165-72 [PMID:10737749]
ChEMBL	Antagonist activity against adenosine A1 receptor in rat brain membrane in presence of [3H]R-PIA radioligand.	F	7.77	pKi	16.8	nM	Ki	J. Med. Chem. (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Binding affinity towards rat Adenosine A1 receptor	B	7.77	pKi	16.8	nM	Ki	J. Med. Chem. (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Binding affinity of specific [3H]R-PIA binding to rat Adenosine A1 receptor in HEK293 cells	B	7.77	pKi	16.8	nM	Ki	J. Med. Chem. (2002) 45: 2131-2138 [PMID:12014951]
ChEMBL	Binding affinity to rat adenosine A1 receptor	B	7.77	pKi	16.8	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Displacement of [3H]R-PIA from A1 adenosine receptor in rat brain membranes measured after 3 hrs in presence of adenosine deaminase	B	7.77	pKi	16.8	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A2A receptor/Adenosine A2a receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274]
ChEMBL	The A2A-StaR2 receptor has been purified from membranes in n-decyl-beta-D-maltoside as detergent. Ligands were prepared over a five-fold concentration series and injected in either a multi- or a single-cycle format depending on the kinetic parameters of the ligand. The actual concentrations as well as contact and dissociation times varied between the ligands depended on the kinetics and affinity of the interaction. The data were fitted to a 1:1 interaction model using the kinetics evaluation software from Biacore. A minimum of three measurements were recorded for each interaction.	B	7.25	pKd	55.63	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	Binding affinity to recombinant human adenosine A2A receptor	B	6.29	pKi	512.86	nM	Ki	J. Med. Chem. (2006) 49: 282-299 [PMID:16392813]
ChEMBL	Binding affinity towards human Adenosine A2A receptor	B	6.3	pKi	503	nM	Ki	J. Med. Chem. (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Antagonist activity against recombinant human adenosine A2A receptor expressed in HEK293 cells in presence of [125I]iodo-ZM-241385 radioligand.	F	6.3	pKi	503	nM	Ki	J. Med. Chem. (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Binding affinity to human adenosine A2A receptor	B	6.3	pKi	503	nM	Ki	J. Med. Chem. (2012) 55: 797-811 [PMID:22148859]
ChEMBL	Displacement of [125I]ZM241385 from recombinant human A2A adenosine receptor expressed in HEK293 cell membranes measured after 3 hrs	B	6.3	pKi	503	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
ChEMBL	Binding affinity to human adenosine A2A receptor	B	6.3	pKi	503	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Antagonist activity against human adenosine A2A receptor	F	6.3	pKi	503	nM	Ki	Eur. J. Med. Chem. (2010) 45: 3459-3471 [PMID:20537438]
GtoPdb	-	-	6.3	pKi	503	nM	Ki	J Med Chem (2000) 43: 1165-72 [PMID:10737749]
ChEMBL	Displacement of [3H]ZM-241385 from human adenosine A2a receptor expressed in HEK cells	B	6.3	pKi	500	nM	Ki	J. Med. Chem. (2006) 49: 3682-3692 [PMID:16759111]
ChEMBL	Binding affinity against Adenosine A2a receptor labeled by [3H]ZM-241385 in membranes prepared from HEK-A2A cells	B	6.59	pKi	>260	nM	Ki	Bioorg. Med. Chem. Lett. (2005) 15: 609-612 [PMID:15664822]
ChEMBL	Inhibition of A2aR (unknown origin) expressed in HEK293 cells incubated overnight by dual luciferase reporter assay	B	6	pIC50	1000	nM	IC50	US-20200345725-A1. Methods and Compositions for Treating Cancer (null)
A2A receptor/Adenosine A2a receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2115] [GtoPdb: 19] [UniProtKB: Q60613]
ChEMBL	Displacement of [3H]MSX-2 from recombinant mouse A2A adenosine receptor expressed in CHO cell membranes measured after 60 mins by liquid scintillation counting method	B	5	pKi	>10000	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A2A receptor/Adenosine A2a receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543]
GtoPdb	-	-	6.21	pKi	612	nM	Ki	J Med Chem (2000) 43: 1165-72 [PMID:10737749]
ChEMBL	Antagonist activity against adenosine A2A receptor in rat striatal membrane in presence of [3H]CGS-21680 radioligand.	F	6.21	pKi	612	nM	Ki	J. Med. Chem. (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Binding affinity towards rat Adenosine A2A receptor	B	6.21	pKi	612	nM	Ki	J. Med. Chem. (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Binding affinity at rat Adenosine A2A receptor by [3H]-CGS- 21680 displacement.	B	6.21	pKi	612	nM	Ki	J. Med. Chem. (2002) 45: 2131-2138 [PMID:12014951]
ChEMBL	Binding affinity to rat adenosine A2A receptor	B	6.21	pKi	612	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Displacement of [3H]CGS21680 from A2A adenosine receptor in rat striatal membranes measured after 3 hrs in presence of adenosine deaminase	B	6.21	pKi	612	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A2B receptor/Adenosine A2b receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275]
ChEMBL	Displacement of [3H]DPCPX from human adenosine A2B receptor expressed in HEK293 cells after 30 mins by liquid scintillation counting	B	7.98	pKi	10.39	nM	Ki	Eur. J. Med. Chem. (2012) 54: 914-918 [PMID:22703703]
ChEMBL	Displacement of [3H]ZM241385 from human adenosine A2b receptor expressed in HEK cells	B	8.7	pKi	2	nM	Ki	J. Med. Chem. (2006) 49: 3682-3692 [PMID:16759111]
ChEMBL	Binding affinity to human adenosine A2B receptor	B	8.7	pKi	2	nM	Ki	J. Med. Chem. (2012) 55: 797-811 [PMID:22148859]
ChEMBL	Inhibition of human A2B adenosine receptor	B	8.7	pKi	2	nM	Ki	Eur J Med Chem (2019) 163: 763-778 [PMID:30576906]
ChEMBL	Binding affinity against Adenosine A2b receptor labeled by [3H]ZM-241385 in membranes prepared from HEK-A2B cells	B	8.7	pKi	2	nM	Ki	Bioorg. Med. Chem. Lett. (2005) 15: 609-612 [PMID:15664822]
ChEMBL	Displacement of [125I]ABOPX from human adenosine receptor A2b expressed in HEK293 cell membranes after 3 hrs by fluorescence assay	B	8.7	pKi	2	nM	Ki	Eur J Med Chem (2017) 127: 986-996 [PMID:27842891]
ChEMBL	Binding affinity to recombinant human adenosine A2B receptor	B	8.7	pKi	2	nM	Ki	J. Med. Chem. (2006) 49: 282-299 [PMID:16392813]
ChEMBL	Antagonist activity at human A2B receptor	B	8.71	pKi	1.97	nM	Ki	J Med Chem (2021) 64: 458-480 [PMID:33372800]
ChEMBL	Binding affinity towards human Adenosine A2B receptor	B	8.71	pKi	1.97	nM	Ki	J. Med. Chem. (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Binding affinity to human adenosine A2B receptor	B	8.71	pKi	1.97	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Antagonist activity against human adenosine A2B receptor	F	8.71	pKi	1.97	nM	Ki	Eur. J. Med. Chem. (2010) 45: 3459-3471 [PMID:20537438]
ChEMBL	Displacement of [125I]IABOPX from human recombinant Adenosine A2B receptor expressed in HEK293 cells after 3 hrs	B	8.71	pKi	1.97	nM	Ki	J. Med. Chem. (2016) 59: 1967-1983 [PMID:26824742]
ChEMBL	Displacement of [3H]ZM241385/[125I]IABOPX from recombinant human A2B adenosine receptor expressed in HEK293 cell membranes measured after 3 hrs	B	8.71	pKi	1.97	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
ChEMBL	Antagonist activity against human A2B adenosine receptor expressed in HEK293 cells uisng [3H]ZM-241385 or [125I]-IABOPX	F	8.71	pKi	1.97	nM	Ki	J. Med. Chem. (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Displacement of [3H]ZM241385/[125I]IABOPX from human recombinant adenosine A2B receptor expressed in HEK293 cells after 3 hrs	B	8.72	pKi	1.9	nM	Ki	J Med Chem (2017) 60: 3372-3382 [PMID:28368607]
GtoPdb	-	-	8.8	pKi	1.58	nM	Ki	Biochem Pharmacol (2001) 61: 657-63 [PMID:11266650]; J Med Chem (2000) 43: 1165-72 [PMID:10737749]
ChEMBL	Inhibition of A2bR (unknown origin) expressed in HEK293 cells incubated overnight by dual luciferase reporter assay	B	7.49	pIC50	32	nM	IC50	US-20200345725-A1. Methods and Compositions for Treating Cancer (null)
ChEMBL	Antagonist activity at Adenosine receptor subtype 2B in human RBC assessed as change in erythrocyte morphology incubated for 3 hrs with occasional gentle stirring for every 30 min measured for every 12 hrs by microscopic analysis	F	7.63	pEC50	23.44	nM	EC50	Eur J Med Chem (2017) 136: 487-496 [PMID:28528302]
A2B receptor/Adenosine A2b receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2237] [GtoPdb: 20] [UniProtKB: Q60614]
ChEMBL	Binding affinity to mouse adenosine A2B receptor	B	8.47	pKi	3.39	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
GtoPdb	-	-	8.47	pKi	3.39	nM	Ki	J Pharmacol Exp Ther (2009) 329: 2-13 [PMID:19141710]
ChEMBL	Displacement of [3H]PSB-603 from recombinant mouse A2B adenosine receptor expressed in CHO cell membranes measured after 75 mins by liquid scintillation counting method	B	8.51	pKi	3.12	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A2B receptor/Adenosine A2b receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2592] [GtoPdb: 20] [UniProtKB: P29276]
GtoPdb	-	-	7.89	pKi	12.8	nM	Ki	J Med Chem (2000) 43: 1165-72 [PMID:10737749]; Eur J Pharmacol (2003) 475: 79-84 [PMID:12954362]
ChEMBL	Inhibition of [125I]-APOBX binding to rat Adenosine A2B receptor expressed in HEK cells	B	7.89	pKi	12.8	nM	Ki	J. Med. Chem. (2002) 45: 2131-2138 [PMID:12014951]
ChEMBL	Binding affinity to rat adenosine A2B receptor	B	7.89	pKi	12.8	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Binding affinity to rat A2B adenosine receptor by radio-ligand displacement assay	B	7.89	pKi	12.8	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A3 receptor/Adenosine A3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8]
GtoPdb	-	-	6.24	pKi	570	nM	Ki	J Med Chem (2000) 43: 1165-72 [PMID:10737749]
ChEMBL	Binding affinity to recombinant human adenosine A3 receptor	B	6.24	pKi	575.44	nM	Ki	J. Med. Chem. (2006) 49: 282-299 [PMID:16392813]
ChEMBL	Binding affinity for human Adenosine A3 receptor	B	6.24	pKi	570	nM	Ki	J. Med. Chem. (2002) 45: 1500-1510 [PMID:11906291]
ChEMBL	Displacement of [125I]IABA from recombinant human A3 adenosine receptor expressed in HEK293 cell membranes measured after 3 hrs	B	6.24	pKi	570	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
ChEMBL	Antagonist activity against human adenosine A3 receptor expressed in HEK cells in presence of [125]IAB-MECA or [125I]IABA radioligand.	F	6.24	pKi	570	nM	Ki	J. Med. Chem. (2000) 43: 1165-1172 [PMID:10737749]
ChEMBL	Displacement of [125I]AB-MECA from human adenosine A3 receptor expressed in CHO cells	B	6.24	pKi	570	nM	Ki	J. Med. Chem. (2006) 49: 3682-3692 [PMID:16759111]
ChEMBL	Binding affinity to human adenosine A3 receptor	B	6.24	pKi	570	nM	Ki	J. Med. Chem. (2009) 52: 3994-4006 [PMID:19569717]
ChEMBL	Antagonist activity against human adenosine A3 receptor	F	6.24	pKi	570	nM	Ki	Eur. J. Med. Chem. (2010) 45: 3459-3471 [PMID:20537438]
ChEMBL	Binding affinity to human adenosine A3 receptor	B	6.24	pKi	570	nM	Ki	J. Med. Chem. (2012) 55: 797-811 [PMID:22148859]
ChEMBL	Binding affinity against Adenosine A3 receptor by displacement of specific binding of [125I]AB-MECA in membranes prepared from CHO-A3 cells	B	6.54	pKi	>290	nM	Ki	Bioorg. Med. Chem. Lett. (2005) 15: 609-612 [PMID:15664822]
A3 receptor/Adenosine A3 receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075269] [GtoPdb: 21] [UniProtKB: Q61618]
ChEMBL	Displacement of [3H]NECA from recombinant mouse A3 adenosine receptor expressed in CHO cell membranes measured after 180 mins by liquid scintillation counting method	B	5	pKi	>10000	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]
A3 receptor/Adenosine A3 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647]
ChEMBL	Binding affinity to rat A3 adenosine receptor	B	5	pKi	>10000	nM	Ki	J Med Chem (2019) 62: 4032-4055 [PMID:30835463]

ChEMBL data shown on this page come from version 31:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]