ChEMBL ligand: CHEMBL12089 (Berberine chloride, Berberine hydrochloride, Berberinum, GNF-Pf-4545, NSC-163088, NSC-646666)
Acetylcholinesterase in Electrophorus electricus [ChEMBL: CHEMBL4078] [UniProtKB: O42275] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303] There should be some charts here, you may need to enable JavaScript!
α2B-adrenoceptor/Alpha-2b adrenergic receptor in Human [ChEMBL: CHEMBL1942] [GtoPdb: 26] [UniProtKB: P18089] There should be some charts here, you may need to enable JavaScript!
α2C-adrenoceptor/Alpha-2c adrenergic receptor in Human [ChEMBL: CHEMBL1916] [GtoPdb: 27] [UniProtKB: P18825] There should be some charts here, you may need to enable JavaScript!
butyrylcholinesterase/Butyrylcholinesterase in Human [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276] Butyrylcholinesterase in Equus caballus [ChEMBL: CHEMBL5077] [UniProtKB: Q9N1N9] There should be some charts here, you may need to enable JavaScript!
Cell division protein ftsZ in Escherichia coli [ChEMBL: CHEMBL3999] [UniProtKB: P0A9A6] There should be some charts here, you may need to enable JavaScript!
Cholinesterase in Equus caballus [ChEMBL: CHEMBL5763] [UniProtKB: P81908] There should be some charts here, you may need to enable JavaScript!
CYP2D6/Cytochrome P450 2D6 in Human [ChEMBL: CHEMBL289] [GtoPdb: 1329] [UniProtKB: P10635] There should be some charts here, you may need to enable JavaScript!
Kv11.1/HERG in Human [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] There should be some charts here, you may need to enable JavaScript!
M1 receptor/Muscarinic acetylcholine receptor M1 in Human [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229] There should be some charts here, you may need to enable JavaScript!
NAD(+) hydrolase SARM1 in Human [ChEMBL: CHEMBL4523350] [UniProtKB: Q6SZW1] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum (isolate K1 / Thailand) in Plasmodium falciparum K1 [ChEMBL: CHEMBL612856] There should be some charts here, you may need to enable JavaScript!
Plasmodium yoelii [ChEMBL: CHEMBL612889] There should be some charts here, you may need to enable JavaScript!
Retinoid X receptor-α/Retinoid X receptor alpha in Human [ChEMBL: CHEMBL2061] [GtoPdb: 610] [UniProtKB: P19793] There should be some charts here, you may need to enable JavaScript!
NIMA related kinase 7/Serine/threonine-protein kinase NEK7 in Human [ChEMBL: CHEMBL4849] [GtoPdb: 2122] [UniProtKB: Q8TDX7] There should be some charts here, you may need to enable JavaScript!
5-HT2B receptor/Serotonin 2b (5-HT2b) receptor in Human [ChEMBL: CHEMBL1833] [GtoPdb: 7] [UniProtKB: P41595] There should be some charts here, you may need to enable JavaScript!
Serotonin 4 (5-HT4) receptor in Guinea pig [ChEMBL: CHEMBL5017] [UniProtKB: O70528] There should be some charts here, you may need to enable JavaScript!
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Human [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Acetylcholinesterase in Electrophorus electricus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4078] [UniProtKB: O42275]
ChEMBL	Inhibition of electric eel AChE by enzyme kinetics assay	B	6.54	pKi	289	nM	Ki	Bioorg Med Chem (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	5.56	pIC50	2740	nM	IC50	Bioorg Med Chem (2012) 20: 6669-6679 [PMID:23062825]
ChEMBL	Inhibition of electric eel AChE by Ellman's assay	B	6.43	pIC50	374	nM	IC50	Bioorg Med Chem (2010) 18: 4475-4484 [PMID:20471843]
ChEMBL	Inhibition of electric eel AChE after 15 mins by Ellman's method using acetylcholine as substrate	B	6.43	pIC50	374	nM	IC50	Bioorg Med Chem Lett (2010) 20: 6649-6652 [PMID:20880702]
ChEMBL	Inhibition of electric eel AChE by modified Ellman method	B	6.43	pIC50	374	nM	IC50	Bioorg Med Chem (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL	Inhibition of electric eel AChE using acetylcholine chloride as substrate preincubated for 15 mins by Ellman's method	B	6.43	pIC50	374	nM	IC50	Bioorg Med Chem (2011) 19: 7228-7235 [PMID:22041172]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate preincubated for 15 mins prior to substrate addition by Ellman's method	B	6.43	pIC50	374	nM	IC50	Bioorg Med Chem (2013) 21: 5830-5840 [PMID:23932451]
ChEMBL	Inhibition of electric eel Acetylcholinesterase	B	6.43	pIC50	374	nM	IC50	Bioorg Med Chem (2011) 19: 2298-2305 [PMID:21397508]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303]
ChEMBL	Inhibition of recombinant human acetylcholinesterase using acetylthiocholine iodide as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured at 2 mins interval by Ellman's method	B	4.76	pIC50	17400	nM	IC50	Eur J Med Chem (2020) 203: 112593-112593 [PMID:32688201]
ChEMBL	DRUGMATRIX: Acetylcholinesterase enzyme inhibition (substrate: acetylthiocholine)	B	6.11	pIC50	774	nM	IC50	DrugMatrix in vitro pharmacology data
ChEMBL	Inhibition of human AChE by Ellman's method	B	6.21	pIC50	610	nM	IC50	Bioorg Med Chem (2012) 20: 6669-6679 [PMID:23062825]
ChEMBL	Inhibition of AChE assessed as hydrolysis of acetylcholine preincubated for 15 mins measured after 15 mins by colorimetric Ellman assay	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem Lett (2011) 21: 6603-6607 [PMID:21924611]
α2B-adrenoceptor/Alpha-2b adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1942] [GtoPdb: 26] [UniProtKB: P18089]
ChEMBL	DRUGMATRIX: Alpha-2B adrenergic receptor radioligand binding (ligand: Rauwolscine)	B	6.56	pKi	274	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	DRUGMATRIX: Alpha-2B adrenergic receptor radioligand binding (ligand: Rauwolscine)	B	6.22	pIC50	601	nM	IC50	DrugMatrix in vitro pharmacology data
α2C-adrenoceptor/Alpha-2c adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1916] [GtoPdb: 27] [UniProtKB: P18825]
ChEMBL	DRUGMATRIX: Adrenergic Alpha-2C radioligand binding (ligand: [3H] MK-912)	B	6.39	pKi	412	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	DRUGMATRIX: Adrenergic Alpha-2C radioligand binding (ligand: [3H] MK-912)	B	5.55	pIC50	2837	nM	IC50	DrugMatrix in vitro pharmacology data
butyrylcholinesterase/Butyrylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276]
ChEMBL	Inhibition of human butyrylcholinesterase in plasma using butyrylthiocholineiodide as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured at 2 mins interval by Ellman's method	B	4.33	pIC50	47200	nM	IC50	Eur J Med Chem (2020) 203: 112593-112593 [PMID:32688201]
ChEMBL	Inhibition of BChE assessed as hydrolysis of butrylcholine preincubated for 15 mins measured after 15 mins by colorimetric Ellman assay	B	4.72	pIC50	18970	nM	IC50	Bioorg Med Chem Lett (2011) 21: 6603-6607 [PMID:21924611]
Butyrylcholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5077] [UniProtKB: Q9N1N9]
ChEMBL	Inhibition of equine BChE after 15 mins by Ellman's method using acetylcholine as substrate	B	4.74	pIC50	18200	nM	IC50	Bioorg Med Chem Lett (2010) 20: 6649-6652 [PMID:20880702]
ChEMBL	Inhibition of equine serum Butyrylcholinesterase	B	4.74	pIC50	18200	nM	IC50	Bioorg Med Chem (2011) 19: 2298-2305 [PMID:21397508]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate preincubated for 15 mins by Ellman's method	B	4.74	pIC50	18200	nM	IC50	Bioorg Med Chem (2011) 19: 7228-7235 [PMID:22041172]
Cell division protein ftsZ in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3999] [UniProtKB: P0A9A6]
ChEMBL	Displacement of bis-ANS from Escherichia coli FtsZ	B	7.64	pKd	23	nM	Kd	J Med Chem (2016) 59: 6975-6998 [PMID:26756351]
ChEMBL	Inhibition of recombinant Escherichia coli K12 FtsZ GTPase activity by malachite green sodium molybdate assay	B	4.8	pIC50	16000	nM	IC50	Bioorg Med Chem (2016) 24: 6354-6369 [PMID:27189886]
ChEMBL	Inhibition of recombinant Escherichia coli K12 FtsZ assembly by light-scattering assay	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem (2016) 24: 6354-6369 [PMID:27189886]
Cholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5763] [UniProtKB: P81908]
ChEMBL	Inhibition of horse serum BChE by enzyme kinetics assay	B	5.04	pKi	9200	nM	Ki	Bioorg Med Chem (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL	Inhibition of equine serum BChE using butylthiocholine chloride as substrate preincubated for 15 mins prior to substrate addition by Ellman's method	B	4.74	pIC50	18210	nM	IC50	Bioorg Med Chem (2013) 21: 5830-5840 [PMID:23932451]
ChEMBL	Inhibition of horse serum BChE by Ellman's method	B	4.74	pIC50	18200	nM	IC50	Bioorg Med Chem (2010) 18: 1244-1251 [PMID:20056426]
ChEMBL	Inhibition of equine serum BuChE by Ellman's method	B	4.74	pIC50	18200	nM	IC50	Bioorg Med Chem (2010) 18: 4475-4484 [PMID:20471843]
CYP2D6/Cytochrome P450 2D6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL289] [GtoPdb: 1329] [UniProtKB: P10635]
ChEMBL	DRUGMATRIX: CYP450, 2D6 enzyme inhibition (substrate: 3-Cyano-7-ethoxycoumarin)	B	5.35	pIC50	4504.2	nM	IC50	DrugMatrix in vitro pharmacology data
GtoPdb	-	-	5.4	pIC50	-	-	-	Pharmaceutics (2020) 12: [PMID:32987920]
Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL	Inhibition of human ERG by patch clamp assay	B	5.51	pIC50	3100	nM	IC50	Eur J Med Chem (2017) 140: 448-464 [PMID:28987606]
M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229]
ChEMBL	DRUGMATRIX: Muscarinic M1 radioligand binding (ligand: [3H] N-Methylscopolamine)	B	6.13	pKi	742	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	DRUGMATRIX: Muscarinic M1 radioligand binding (ligand: [3H] N-Methylscopolamine)	B	5.51	pIC50	3080	nM	IC50	DrugMatrix in vitro pharmacology data
NAD(+) hydrolase SARM1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523350] [UniProtKB: Q6SZW1]
ChEMBL	Inhibition of recombinant human SARM1 SAM1-2-TIR domains (412 to 724 residues) expressed in Expi293 cells lysates using ENAD as substrate preincubated for 20 mins followed by ENAD addition and measured at 15 sec interval for 1 hr	B	4.11	pIC50	77000	nM	IC50	Bioorg Med Chem (2020) 28: 115644-115644 [PMID:32828421]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	NOVARTIS: Inhibition of Plasmodium falciparum W2 (drug-resistant) proliferation in erythrocyte-based infection assay	F	6.35	pEC50	442	nM	EC50	Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783]
ChEMBL	NOVARTIS: Inhibition of Plasmodium falciparum 3D7 (drug-susceptible) proliferation in erythrocyte-based infection assay	F	6.66	pEC50	218	nM	EC50	Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783]
Plasmodium falciparum (isolate K1 / Thailand) in Plasmodium falciparum K1 (target type: ORGANISM) [ChEMBL: CHEMBL612856]
ChEMBL	Antiplasmodial activity against multi drug-resistant Plasmodium falciparum K1 in erythrocytes by [3H]hypoxanthine uptake	F	6.01	pIC50	968	nM	IC50	J Nat Prod (2000) 63: 1638-1640 [PMID:11141105]
Plasmodium yoelii (target type: ORGANISM) [ChEMBL: CHEMBL612889]
ChEMBL	NOVARTIS: Antimalarial liver stage activity measured as reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells by immuno-fluorescence, and median schizont size at 10uM compound concentration	F	6.26	pIC50	548	nM	IC50	Science (2011) 334: 1372-1377 [PMID:22096101]
Retinoid X receptor-α/Retinoid X receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2061] [GtoPdb: 610] [UniProtKB: P19793]
ChEMBL	Binding affinity to His-tagged RXRalpha LBD (unknown origin) expressed in Escherichia coli strain BL21 (DE3) by fluorescence quenching analysis	B	4.61	pKd	24800	nM	Kd	J Med Chem (2020) 63: 5841-5855 [PMID:32391701]
NIMA related kinase 7/Serine/threonine-protein kinase NEK7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4849] [GtoPdb: 2122] [UniProtKB: Q8TDX7]
ChEMBL	Binding affinity to purified recombinant human NEK7 by SPR analysis	B	4.81	pKd	15600	nM	Kd	J Med Chem (2021) 64: 768-781 [PMID:33440945]
ChEMBL	Inhibition of purified recombinant human NEK7 incubated for 15 mins before ATP and substrate addition and further incubated for 40 mins by ADPGlo kinase assay	B	5.38	pIC50	4200	nM	IC50	J Med Chem (2021) 64: 768-781 [PMID:33440945]
5-HT2B receptor/Serotonin 2b (5-HT2b) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1833] [GtoPdb: 7] [UniProtKB: P41595]
ChEMBL	DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT2B radioligand binding (ligand: [3H] Lysergic acid diethylamide)	B	5.66	pKi	2187	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT2B radioligand binding (ligand: [3H] Lysergic acid diethylamide)	B	5.46	pIC50	3437	nM	IC50	DrugMatrix in vitro pharmacology data
Serotonin 4 (5-HT4) receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5017] [UniProtKB: O70528]
ChEMBL	DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT4 radioligand binding (ligand: [3H] GR-113808)	B	6.43	pKi	370	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	DRUGMATRIX: Serotonin (5-Hydroxytryptamine) 5-HT4 radioligand binding (ligand: [3H] GR-113808)	B	5.65	pIC50	2222	nM	IC50	DrugMatrix in vitro pharmacology data
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720]
ChEMBL	DRUGMATRIX: Sigma1 radioligand binding (ligand: [3H] Haloperidol)	B	6.27	pKi	542	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	DRUGMATRIX: Sigma1 radioligand binding (ligand: [3H] Haloperidol)	B	5.89	pIC50	1289	nM	IC50	DrugMatrix in vitro pharmacology data

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]