Top ▲

GtoPdb is requesting financial support from commercial users. Please see our sustainability page for more information.

Kir3.3

Click here for help

Target not currently curated in GtoImmuPdb

Target id: 436

Nomenclature: Kir3.3

Family: Inwardly rectifying potassium channels (KIR)

Contents:

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 2 1 393 1q23.2 KCNJ9 potassium inwardly rectifying channel subfamily J member 9 20
Mouse 2 1 393 1 79.66 cM Kcnj9 potassium inwardly-rectifying channel, subfamily J, member 9 13,19,29
Rat 2 1 393 13q24 Kcnj9 potassium inwardly-rectifying channel, subfamily J, member 9 6,28
Previous and Unofficial Names Click here for help
potassium inwardly rectifying channel subfamily J member 9 | GIRK3 | G protein-activated inward rectifier potassium channel 3 | inward rectifier K(+) channel Kir3.3 | potassium channel, inwardly rectifying subfamily J, member 9 | potassium inwardly-rectifying channel | potassium voltage-gated channel subfamily J member 9
Database Links Click here for help
Alphafold Q92806 (Hs), P48543 (Mm), Q63511 (Rn)
CATH/Gene3D 2.60.40.1400
ChEMBL Target CHEMBL3038490 (Hs)
Ensembl Gene ENSG00000162728 (Hs), ENSMUSG00000038026 (Mm), ENSRNOG00000007645 (Rn)
Entrez Gene 3765 (Hs), 16524 (Mm), 116560 (Rn)
Human Protein Atlas ENSG00000162728 (Hs)
KEGG Gene hsa:3765 (Hs), mmu:16524 (Mm), rno:116560 (Rn)
OMIM 600932 (Hs)
Pharos Q92806 (Hs)
RefSeq Nucleotide NM_004983 (Hs), NM_008429 (Mm), NM_053834 (Rn)
RefSeq Protein NP_004974 (Hs), NP_032455 (Mm), NP_446286 (Rn)
UniProtKB Q92806 (Hs), P48543 (Mm), Q63511 (Rn)
Wikipedia KCNJ9 (Hs)
Associated Proteins Click here for help
Heteromeric Pore-forming Subunits
Name References
Kir3.1 11
Kir3.2 10
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
SNX27 21
regulator of G-protein signaling 2 17
Associated Protein Comments
Trafficking and expression:
  • Kir3.3 contains lysosomal targeting motif [23].
  • Kir3.3 contains PDZ binding motif that binds PDZ domain of SNX27 [3,21].
  • Evidence for formation of macromolecular signalling complexes containing Kir3.3 [4,7-8].
  • Surface expression of Kir3.2/Kir3.3 currents regulated by SNX27 in VTA DA neurons [25].
  • Regulation of Kir3.3-containing channels by SNX27 and Ras-like G proteins [2].
Functional Characteristics Click here for help
G protein-activated inward-rectifier current
Ion Selectivity and Conductance Click here for help
Species:  Mouse
Rank order:  K+
References:  10-11
Ion Selectivity and Conductance Comments
Kir3.3 forms functional heteromers with Kir3.2 (31pS, [10]) and Kir3.1 (39pS, [11,14]).

Download all structure-activity data for this target as a CSV file go icon to follow link

Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand
Sp.
Reference
PIP2 Small molecule or natural product Click here for species-specific activity table Ligand is endogenous in the given species Hs - - - - - 9
⤷ [9]
Activator Comments
Kir3.3 in complex with Kir3.1 is activated by Gβγ subunits (pEC50 = 11 [11]).
Tissue Distribution Click here for help
Pituitary, small intestine, testis, brain, fat, kidney, skeletal muscle, smooth muscle, pancreas.
Species:  Human
Technique:  RT-PCR
References:  28
Brain > skeletal muscle.
Species:  Mouse
Technique:  Northern Blot
References:  19
Brain (cortex, cerebellum, thalamus)
Species:  Mouse
Technique:  In situ hybridisation
References:  13
Cerebellum: Kir3.3 expression in Purkinje neurons (GIRK1/GIRK2/GIRK3), basket cells (GIRK1/GIRK3), stellate cells (GIRK3), and unipolar brush cells (GIRK2/GIRK3).
Species:  Mouse
Technique: 
References:  1
Brain (neocortex, olfactory system, hippocampus, thalamus > cerebellum, midbrain nuclei, brainstem nuclei, hypothalamus, amygdala, caudate putamen)
Species:  Rat
Technique:  In situ hybridisation
References:  6,12
Physiological Functions Click here for help
Receptor-dependent hyperpolarisation of membrane potential.
Species:  None
Tissue:  Heterologous expression in Xenopus oocytes
References:  6,10-11
Functional Kir3.2 and Kir3.3 heterotetramers in regulate neuronal excitability
Species:  Mouse
Tissue:  VTA and LC
References:  5,27
Decreased cocaine self-administration in mice lacking Kir3.3.
Species:  Mouse
Tissue: 
References:  24
Contribution of Kir3.3 to GABAB receptor activated current
Species:  Mouse
Tissue:  Hippocampus
References:  15
Contribution of Kir3.3 to GABAB receptor currents in ventral tegmental area dopaminergic neurons affecting response to γ-hydroxybutyric acid
Species:  Mouse
Tissue:  Ventral tegmental area
References:  5,17
Channels containing Kir3.2 and Kir3.3 serve as an inhibitory gate in the locus coeruleus regulating morphine withdrawal
Species:  Mouse
Tissue:  Locus coeruleus
References:  15
Role for Kir3.3 in withdrawal from ethanol, pentobarbital and zolpidem
Species:  Mouse
Tissue: 
References:  16
Activity dependent plasticity in GABAB-GIRK currents in ventral tegmental area dopaminergic neurons
Species:  Mouse
Tissue:  Ventral tegmental area
References:  18
Kir3.3 knockout mice behave normally in open-field motor activity and habituation, anxiety-related behavior, motor co-ordination and ataxia and operant performance
Species:  Mouse
Tissue: 
References:  26
Physiological Functions Comments
For review see [22].
Phenotypes, Alleles and Disease Models Click here for help Mouse data from MGI

Show »

Allele
Composition & genetic background
Accession
Phenotype Id
Phenotype
Reference
Kcnj9tm1Kwn Kcnj9tm1Kwn/Kcnj9tm1Kwn
involves: 129X1/SvJ		 MGI:108007  MP:0003635 abnormal synaptic transmission PMID: 12040038 
Kcnj6tm1Stf|Kcnj9tm1Kwn Kcnj6tm1Stf/Kcnj6tm1Stf,Kcnj9tm1Kwn/Kcnj9tm1Kwn
involves: 129S1/Sv * 129X1/SvJ		 MGI:104781  MGI:108007  MP:0003635 abnormal synaptic transmission PMID: 12040038 
Kcnj6tm1Stf|Kcnj9tm1Kwn Kcnj6tm1Stf/Kcnj6tm1Stf,Kcnj9tm1Kwn/Kcnj9tm1Kwn
involves: 129S1/Sv * 129X1/SvJ		 MGI:104781  MGI:108007  MP:0002083 premature death PMID: 12040038 
Kcnj6tm1Stf|Kcnj9tm1Kwn Kcnj6tm1Stf/Kcnj6tm1Stf,Kcnj9tm1Kwn/Kcnj9tm1Kwn
involves: 129S1/Sv * 129X1/SvJ		 MGI:104781  MGI:108007  MP:0002064 seizures PMID: 12040038 
Clinically-Relevant Mutations and Pathophysiology Click here for help
Disease:  Diabetes mellitus, noninsulin-dependent; NIDDM
Synonyms: Diabetes mellitus, Type II; T2D [OMIM: 125853]
Maturity onset diabetes
Type 2 diabetes mellitus [Disease Ontology: DOID:9352]
Disease Ontology: DOID:9352
OMIM: 125853
References:  28

References

Show »

1. Aguado C, Colón J, Ciruela F, Schlaudraff F, Cabañero MJ, Perry C, Watanabe M, Liss B, Wickman K, Luján R. (2008) Cell type-specific subunit composition of G protein-gated potassium channels in the cerebellum. J Neurochem, 105 (2): 497-511. [PMID:18088366]

2. Balana B, Bahima L, Bodhinathan K, Taura JJ, Taylor NM, Nettleton MY, Ciruela F, Slesinger PA. (2013) Ras-association domain of sorting Nexin 27 is critical for regulating expression of GIRK potassium channels. PLoS ONE, 8 (3): e59800. [PMID:23536889]

3. Balana B, Maslennikov I, Kwiatkowski W, Stern KM, Bahima L, Choe S, Slesinger PA. (2011) Mechanism underlying selective regulation of G protein-gated inwardly rectifying potassium channels by the psychostimulant-sensitive sorting nexin 27. Proc Natl Acad Sci USA, 108 (14): 5831-6. [PMID:21422294]

4. Ciruela F, Fernández-Dueñas V, Sahlholm K, Fernández-Alacid L, Nicolau JC, Watanabe M, Luján R. (2010) Evidence for oligomerization between GABAB receptors and GIRK channels containing the GIRK1 and GIRK3 subunits. Eur J Neurosci, 32 (8): 1265-77. [PMID:20846323]

5. Cruz HG, Ivanova T, Lunn ML, Stoffel M, Slesinger PA, Lüscher C. (2004) Bi-directional effects of GABA(B) receptor agonists on the mesolimbic dopamine system. Nat Neurosci, 7 (2): 153-9. [PMID:14745451]

6. Dissmann E, Wischmeyer E, Spauschus A, Pfeil DV, Karschin C, Karschin A. (1996) Functional expression and cellular mRNA localization of a G protein-activated K+ inward rectifier isolated from rat brain. Biochem Biophys Res Commun, 223 (2): 474-9. [PMID:8670306]

7. Fernández-Alacid L, Aguado C, Ciruela F, Martín R, Colón J, Cabañero MJ, Gassmann M, Watanabe M, Shigemoto R, Wickman K et al.. (2009) Subcellular compartment-specific molecular diversity of pre- and post-synaptic GABA-activated GIRK channels in Purkinje cells. J Neurochem, 110 (4): 1363-76. [PMID:19558451]

8. Fowler CE, Aryal P, Suen KF, Slesinger PA. (2007) Evidence for association of GABA(B) receptors with Kir3 channels and regulators of G protein signalling (RGS4) proteins. J Physiol (Lond.), 580 (Pt 1): 51-65. [PMID:17185339]

9. Hilgemann DW, Ball R. (1996) Regulation of cardiac Na+,Ca2+ exchange and KATP potassium channels by PIP2. Science, 273 (5277): 956-9. [PMID:8688080]

10. Jelacic TM, Kennedy ME, Wickman K, Clapham DE. (2000) Functional and biochemical evidence for G-protein-gated inwardly rectifying K+ (GIRK) channels composed of GIRK2 and GIRK3. J Biol Chem, 275 (46): 36211-6. [PMID:10956667]

11. Jelacic TM, Sims SM, Clapham DE. (1999) Functional expression and characterization of G-protein-gated inwardly rectifying K+ channels containing GIRK3. J Membr Biol, 169 (2): 123-9. [PMID:10341034]

12. Karschin C, Karschin A. (1997) Ontogeny of gene expression of Kir channel subunits in the rat. Mol Cell Neurosci, 10 (3-4): 131-48. [PMID:9532576]

13. Kobayashi T, Ikeda K, Ichikawa T, Abe S, Togashi S, Kumanishi T. (1995) Molecular cloning of a mouse G-protein-activated K+ channel (mGIRK1) and distinct distributions of three GIRK (GIRK1, 2 and 3) mRNAs in mouse brain. Biochem Biophys Res Commun, 208 (3): 1166-73. [PMID:7702616]

14. Kofuji P, Davidson N, Lester HA. (1995) Evidence that neuronal G-protein-gated inwardly rectifying K+ channels are activated by G beta gamma subunits and function as heteromultimers. Proc Natl Acad Sci USA, 92 (14): 6542-6. [PMID:7604029]

15. Koyrakh L, Luján R, Colón J, Karschin C, Kurachi Y, Karschin A, Wickman K. (2005) Molecular and cellular diversity of neuronal G-protein-gated potassium channels. J Neurosci, 25 (49): 11468-78. [PMID:16339040]

16. Kozell LB, Walter NA, Milner LC, Wickman K, Buck KJ. (2009) Mapping a barbiturate withdrawal locus to a 0.44 Mb interval and analysis of a novel null mutant identify a role for Kcnj9 (GIRK3) in withdrawal from pentobarbital, zolpidem, and ethanol. J Neurosci, 29 (37): 11662-73. [PMID:19759313]

17. Labouèbe G, Lomazzi M, Cruz HG, Creton C, Luján R, Li M, Yanagawa Y, Obata K, Watanabe M, Wickman K et al.. (2007) RGS2 modulates coupling between GABAB receptors and GIRK channels in dopamine neurons of the ventral tegmental area. Nat Neurosci, 10 (12): 1559-68. [PMID:17965710]

18. Lalive AL, Munoz MB, Bellone C, Slesinger PA, Lüscher C, Tan KR. (2014) Firing modes of dopamine neurons drive bidirectional GIRK channel plasticity. J Neurosci, 34 (15): 5107-14. [PMID:24719090]

19. Lesage F, Duprat F, Fink M, Guillemare E, Coppola T, Lazdunski M, Hugnot JP. (1994) Cloning provides evidence for a family of inward rectifier and G-protein coupled K+ channels in the brain. FEBS Lett, 353 (1): 37-42. [PMID:7926018]

20. Lesage F, Fink M, Barhanin J, Lazdunski M, Mattéi MG. (1995) Assignment of human G-protein-coupled inward rectifier K+ channel homolog GIRK3 gene to chromosome 1q21-q23. Genomics, 29 (3): 808-9. [PMID:8575783]

21. Lunn ML, Nassirpour R, Arrabit C, Tan J, McLeod I, Arias CM, Sawchenko PE, Yates 3rd JR, Slesinger PA. (2007) A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction. Nat Neurosci, 10 (10): 1249-59. [PMID:17828261]

22. Lüscher C, Slesinger PA. (2010) Emerging roles for G protein-gated inwardly rectifying potassium (GIRK) channels in health and disease. Nat Rev Neurosci, 11 (5): 301-15. [PMID:20389305]

23. Ma D, Zerangue N, Raab-Graham K, Fried SR, Jan YN, Jan LY. (2002) Diverse trafficking patterns due to multiple traffic motifs in G protein-activated inwardly rectifying potassium channels from brain and heart. Neuron, 33 (5): 715-29. [PMID:11879649]

24. Morgan AD, Carroll ME, Loth AK, Stoffel M, Wickman K. (2003) Decreased cocaine self-administration in Kir3 potassium channel subunit knockout mice. Neuropsychopharmacology, 28 (5): 932-8. [PMID:12637950]

25. Munoz MB, Slesinger PA. (2014) Sorting nexin 27 regulation of G protein-gated inwardly rectifying K⁺ channels attenuates in vivo cocaine response. Neuron, 82 (3): 659-69. [PMID:24811384]

26. Pravetoni M, Wickman K. (2008) Behavioral characterization of mice lacking GIRK/Kir3 channel subunits. Genes Brain Behav, 7 (5): 523-31. [PMID:18194467]

27. Torrecilla M, Marker CL, Cintora SC, Stoffel M, Williams JT, Wickman K. (2002) G-protein-gated potassium channels containing Kir3.2 and Kir3.3 subunits mediate the acute inhibitory effects of opioids on locus ceruleus neurons. J Neurosci, 22 (11): 4328-34. [PMID:12040038]

28. Vaughn J, Wolford JK, Prochazka M, Permana PA. (2000) Genomic structure and expression of human KCNJ9 (Kir3.3/GIRK3). Biochem Biophys Res Commun, 274 (2): 302-9. [PMID:10913335]

29. Wickman K, Pu WT, Clapham DE. (2002) Structural characterization of the mouse Girk genes. Gene, 284 (1-2): 241-50. [PMID:11891065]

Contributors

Show »

How to cite this page

Adelman JP, Clapham DE, Hibino H, Inanobe A, Jan LY, Karschin A, Kubo Y, Kurachi Y, Lazdunski M, Miki T, Nichols CG, Palmer LG, Pearson WL, Sackin H, Seino S, Slesinger PA, Tucker S, Vandenberg CA. Inwardly rectifying potassium channels (KIR) in GtoPdb v.2023.1. IUPHAR/BPS Guide to Pharmacology CITE. 2023; 2023(1). Available from: https://doi.org/10.2218/gtopdb/F74/2023.1.