ChEMBL ligand: CHEMBL278020 (Miostin, Neostigmine, Neostigmine (cation), Neostigmine cation, Neostigmine hydroxide, Neostigmine ion)
Acetylcholinesterase in Torpedo californica [ChEMBL: CHEMBL4780] [UniProtKB: P04058] Acetylcholinesterase in Electrophorus electricus [ChEMBL: CHEMBL4078] [UniProtKB: O42275] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303] There should be some charts here, you may need to enable JavaScript!
butyrylcholinesterase/Butyrylcholinesterase in Human [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276] There should be some charts here, you may need to enable JavaScript!
Cholinesterase in Equus caballus [ChEMBL: CHEMBL5763] [UniProtKB: P81908] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Acetylcholinesterase in Torpedo californica (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4780] [UniProtKB: P04058]
ChEMBL	Inhibition of Torpedo californica eel AChE using acetylthiocholine/DTNB as substrate preincubated for 10 mins followed by substrate addition measured every 30 secs for 30 mins by Ellmans method	B	8.34	pIC50	4.6	nM	IC50	Medchemcomm (2011) 2: 406-412
Acetylcholinesterase in Electrophorus electricus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4078] [UniProtKB: O42275]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	4.55	pIC50	28300	nM	IC50	Bioorg Med Chem (2014) 22: 6163-6173 [PMID:25257911]
ChEMBL	Inhibition of electric eel acetylcholinesterase using acetylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	4.66	pIC50	22000	nM	IC50	Eur J Med Chem (2014) 78: 43-53 [PMID:24675179]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured after 15 mins by Ellman's method	B	4.79	pIC50	16300	nM	IC50	Eur J Med Chem (2019) 180: 656-672 [PMID:31352246]
ChEMBL	Inhibition of electric eel acetylcholinesterase using acetylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	4.85	pIC50	14200	nM	IC50	Eur J Med Chem (2014) 78: 167-177 [PMID:24681981]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	5.12	pIC50	7500	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127505-127505 [PMID:32822761]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303]
ChEMBL	Inhibition of AChE (unknown origin) using ACh chloride as substrate preincubated for 15 mins before substrate addition by Ellman method	B	5.62	pIC50	2400	nM	IC50	Bioorg Med Chem Lett (2014) 24: 3618-3621 [PMID:24909082]
GtoPdb	Inhibition of ACHE activity in human serum.	-	7.35	pIC50	45	nM	IC50	Bioorg Med Chem (2007) 15: 7391-8 [PMID:17888667]
butyrylcholinesterase/Butyrylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	4.31	pIC50	49100	nM	IC50	Eur J Med Chem (2014) 78: 43-53 [PMID:24675179]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	4.58	pIC50	26100	nM	IC50	Eur J Med Chem (2014) 78: 167-177 [PMID:24681981]
ChEMBL	Inhibition of human BChE by Ellman's method	B	4.79	pIC50	16300	nM	IC50	Bioorg Med Chem (2014) 22: 6163-6173 [PMID:25257911]
Cholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5763] [UniProtKB: P81908]
ChEMBL	Inhibition of BuChE in equine serum using butyrylthiocholine chloride as substrate preincubated with enzyme for 10 mins prior to substrate challenge for 15 mins by Ellman's method	B	4.3	pIC50	49600	nM	IC50	Eur J Med Chem (2014) 78: 106-117 [PMID:24681070]
ChEMBL	Inhibition of equine BuChE by Ellman's method	B	5.53	pIC50	2930	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127505-127505 [PMID:32822761]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]