ChEMBL ligand: CHEMBL396778 (EMD 1195686, EMD-1195686, Safinamide, Xadago)
Monoamine oxidase A in Human [ChEMBL: CHEMBL1951] [GtoPdb: 2489] [UniProtKB: P21397] There should be some charts here, you may need to enable JavaScript!
Monoamine oxidase B in Human [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338] Monoamine oxidase B in Rat [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643] There should be some charts here, you may need to enable JavaScript!
Nav1.7/Sodium channel protein type IX alpha subunit in Human [ChEMBL: CHEMBL4296] [GtoPdb: 584] [UniProtKB: Q15858] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Monoamine oxidase A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1951] [GtoPdb: 2489] [UniProtKB: P21397]
ChEMBL	Inhibition of recombinant human MAOA using p-tyramine as substrate after 30 mins by Amplex red based spectrophotometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem (2015) 23: 6486-6496 [PMID:26337020]
ChEMBL	Inhibition of human recombinant MAO-A using pargyline as incubated for 20 mins measuring increase in emission signal at 310 nm multimode plate reader assay	B	4	pIC50	100000	nM	IC50	Bioorg Med Chem (2021) 42: 116255-116255 [PMID:34119696]
ChEMBL	Inhibition of recombinant human MAO-A using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by amplex red reagent based spectrophotometric assay	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem (2018) 26: 232-244 [PMID:29198609]
ChEMBL	Inhibition of recombinant human MAO-A expressed in Sf9 cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition for 60 mins by Luminex assay	B	4.17	pIC50	67410	nM	IC50	J Med Chem (2020) 63: 15021-15036 [PMID:33210537]
ChEMBL	Inhibition of human MAO-A expressed in baculovirus infected insect cell membranes using kynuramine as substrate after 15 to 20 mins by discontinuous fluorimetric method	B	4.3	pIC50	>50000	nM	IC50	Eur J Med Chem (2018) 148: 487-497 [PMID:29477889]
ChEMBL	Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using tyramine as substrate preincubated for 1 hr followed by substrate addition and measured after 30 mins by resazurin dye-based fluorescence assay	B	4.3	pIC50	>50000	nM	IC50	J Med Chem (2018) 61: 7043-7064 [PMID:30016860]
ChEMBL	Inhibition of human brain MAO-A using [14C]-5- hydroxytryptamine creatinine disulphate substrate after 30 mins	B	4.35	pIC50	45000	nM	IC50	J Med Chem (2014) 57: 6679-6703 [PMID:24955776]
ChEMBL	Inhibition of recombinant human MAO-A expressed in Sf9 cells preincubated for 15 min followed by addition of p-tyramine and measured after 60 min by Luminex assay	B	4.55	pIC50	28065	nM	IC50	Eur J Med Chem (2022) 228: 114025-114025 [PMID:34871839]
ChEMBL	Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by resorufin-based fluorescence assay	B	4.59	pIC50	25900	nM	IC50	Eur J Med Chem (2019) 162: 793-809 [PMID:30522087]
ChEMBL	Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells assessed as decrease in H2O2 production using p-tyramine as substrate incubated for 20 mins by horse-radish peroxidase/amplex red-based fluorescence method	B	4.59	pIC50	>25900	nM	IC50	Eur J Med Chem (2019) 179: 404-422 [PMID:31265934]
ChEMBL	Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by horse-radish peroxidase/Amplex Red coupled fluorimetric analysis	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112911-112911 [PMID:33071056]
Monoamine oxidase B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338]
ChEMBL	Binding affinity to human recombinant microsomal MAO-B by ITC	B	6.73	pKd	187.2	nM	Kd	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
ChEMBL	Inhibition of human recombinant MAOB expressed in Pichia pastoris	B	6.35	pKi	450	nM	Ki	J Med Chem (2007) 50: 5848-5852 [PMID:17915852]
GtoPdb	-	-	6.35	pKi	450	nM	Ki	J Med Chem (2004) 47: 1767-74 [PMID:15027868]
ChEMBL	Inhibition of human recombinant microsomal MAO-B expressed in Pichia pastoris incubated for 30 mins prior to substrate addition measured after 60 mins by MAO-Glo assay	B	7.78	pKi	16.7	nM	Ki	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
ChEMBL	Inhibition of human recombinant soluble MAO-B expressed in Pichia pastoris incubated for 30 mins prior to substrate addition measured after 60 mins by MAO-Glo assay	B	8.27	pKi	5.4	nM	Ki	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells assessed as decrease in H2O2 production using p-tyramine as substrate incubated for 20 mins by horse-radish peroxidase/amplex red-based fluorescence method	B	8.64	pKi	2.29	nM	Ki	Eur J Med Chem (2019) 179: 404-422 [PMID:31265934]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by Cheng-Prusoff equation analysis	B	8.64	pKi	2.29	nM	Ki	Eur J Med Chem (2019) 162: 793-809 [PMID:30522087]
ChEMBL	Inhibition of recombinant human MAOB using kynuramine as substrate measured for 30 mins by fluorescence spectrophotometric assay	B	4.6	pIC50	25300	nM	IC50	Eur J Med Chem (2021) 213: 113183-113183 [PMID:33493825]
ChEMBL	Inhibition of MAOB (unknown origin)	B	6.35	pIC50	450	nM	IC50	Medchemcomm (2015) 6: 2146-2157
ChEMBL	Inhibition of recombinant human MAO-B expressed in Sf9 cells using benzylamine as substrate preincubated for 15 mins followed by substrate addition for 60 mins by Luminex assay	B	6.6	pIC50	249	nM	IC50	J Med Chem (2020) 63: 15021-15036 [PMID:33210537]
ChEMBL	Inhibition of recombinant human MAO-B expressed in Sf9 cells preincubated for 15 min followed by addition of benzylamine and measured after 60 min by Luminex assay	B	6.79	pIC50	163	nM	IC50	Eur J Med Chem (2022) 228: 114025-114025 [PMID:34871839]
ChEMBL	Inhibition of recombinant human MAOB using benzylamine as substrate after 30 mins by Amplex red based spectrophotometric analysis	B	6.95	pIC50	112	nM	IC50	Bioorg Med Chem (2015) 23: 6486-6496 [PMID:26337020]
ChEMBL	Inhibition of human MAO-B	B	7.01	pIC50	98	nM	IC50	Bioorg Med Chem Lett (2021) 43: 128051-128051 [PMID:33887441]
ChEMBL	Inhibition of MAOB	B	7.1	pIC50	80	nM	IC50	Eur J Med Chem (2009) 44: 3584-3590 [PMID:19321235]
ChEMBL	Inhibition of human recombinant MAO-B using kynuramine as substrate incubated for 20 mins measuring increase in emission signal at 400 nm multimode plate reader assay	B	7.2	pIC50	63	nM	IC50	Bioorg Med Chem (2021) 42: 116255-116255 [PMID:34119696]
ChEMBL	Inhibition of human MAO-B expressed in baculovirus infected insect cell membranes using kynuramine as substrate after 15 to 20 mins by discontinuous fluorimetric method	B	7.28	pIC50	53	nM	IC50	Eur J Med Chem (2018) 148: 487-497 [PMID:29477889]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cell microsomes using kynuramine as substrate after 20 mins by fluorescence spectroscopy	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2017) 135: 196-203 [PMID:28456030]
ChEMBL	Inhibition of recombinant human MAO-B after 20 mins using 50 uM kynuramine as substrate by fluorescence spectrophotometry	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem Lett (2016) 26: 1200-1204 [PMID:26821818]
ChEMBL	Inhibition of human recombinant MAO-B using kynuramine as substrate incubated for 20 mins by fluorescence spectrophotometric analysis	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2214-2219 [PMID:27020523]
ChEMBL	Inhibition of recombinant human MAOB expressed in insect cell microsomes using kynuramine as substrate measured after 20 mins by fluorescence spectrophotometry	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem Lett (2019) 29: 40-46 [PMID:30455149]
ChEMBL	Inhibition of human recombinant MAO-B expressed in insect cells using p-tyramine as substrate preincubated for 30 mins followed by substrate addition measured for 15 mins by resorufin dye-based fluorescence assay	B	7.41	pIC50	39	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1012-1018 [PMID:30792039]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by horse-radish peroxidase/Amplex Red coupled fluorimetric analysis	B	7.54	pIC50	29	nM	IC50	Eur J Med Chem (2021) 209: 112911-112911 [PMID:33071056]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected insect cell microsomes using kynuramine as substrate measured after 30 mins by spectrophotometric assay	B	7.55	pIC50	28	nM	IC50	Eur J Med Chem (2019) 161: 292-309 [PMID:30366255]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI-TN- 5B1-4 insect cells using kynuramine as substrate preincubated for 10 mins in presence of substrate followed by enzyme addition and measured every minute for 30 mins by spectrophotometry analysis	B	7.6	pIC50	25.3	nM	IC50	J Med Chem (2021) 64: 11169-11182 [PMID:34269579]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells using benzylamine as substrate preincubated for 1 hr followed by substrate addition and measured after 30 mins by resazurin dye-based fluorescence assay	B	7.62	pIC50	24	nM	IC50	J Med Chem (2018) 61: 7043-7064 [PMID:30016860]
ChEMBL	Inhibition of human microsomal MAO-B expressed in baculovirus infected BTI-TN-5B1-4 cells assessed as reduction in 4-hydroxyquinoline formation using kynuramine as substrate preincubated with substrate for 10 mins followed by enzyme addition by spectrophotometric analysis	B	7.64	pIC50	23.1	nM	IC50	Eur J Med Chem (2020) 185: 111770-111770 [PMID:31711793]
ChEMBL	Inhibition of human microsomal MAO-B expressed in recombinant baculovirus infected insect BTI-TN-5B1-4 cells assessed as reduction in H2O2 production using p-tyramine as substrate after 15 mins by fluorescence assay	B	7.64	pIC50	23.07	nM	IC50	J Med Chem (2016) 59: 5879-5893 [PMID:27244485]
ChEMBL	Inhibition of human recombinant microsomal MAOB expressed in baculovirus infected BTI-TN-5B1- 4 cells using p-tyramine as substrate assessed as decrease in H2O2 production after 15 mins by amplex red-based fluorescence assay	B	7.64	pIC50	23.07	nM	IC50	J Med Chem (2017) 60: 7206-7212 [PMID:28753307]
ChEMBL	Inhibition of human recombinant MAOB expressed in baculovirus infected BTI-TN-5B1- 4 cells using p-tyramine as substrate assessed as decrease in H2O2 production by amplex red-based fluorescence assay	B	7.64	pIC50	23	nM	IC50	Eur J Med Chem (2018) 158: 781-800 [PMID:30245401]
ChEMBL	Inhibition of human microsomal MAO-B expressed in recombinant baculovirus infected insect BTI-TN-5B1-4 cells assessed as reduction in H2O2 production using p-tyramine as substrate after 15 mins by fluorescence assay	B	7.64	pIC50	22.91	nM	IC50	J Med Chem (2016) 59: 5879-5893 [PMID:27244485]
ChEMBL	Inhibition of human MAO-B expressed in baculovirus infected insect cell membranes using kynuramine as substrate preincubated for 30 mins followed by substrate addition measured after 15 to 20 mins by discontinuous fluorimetric method	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2018) 148: 487-497 [PMID:29477889]
ChEMBL	Inhibition of human recombinant MAO-B expressed in supersomes assessed as inhibition of 4-hydroxyquinoline formation using kynuramine as substrate by spectrofluorimetric analysis	B	7.74	pIC50	18	nM	IC50	J Med Chem (2022) 65: 3962-3977 [PMID:35195417]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected insect cell microsomes using kynuramine as substrate measured after 30 mins by fluorescence based assay	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2019) 161: 292-309 [PMID:30366255]
ChEMBL	Inhibition of recombinant human MAO-B using benzylamine as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by amplex red reagent based spectrophotometric assay	B	7.77	pIC50	17	nM	IC50	Bioorg Med Chem (2018) 26: 232-244 [PMID:29198609]
ChEMBL	Inhibition of human brain MAO-B using [14C]-phenylethylamine substrate after 20 mins	B	8.05	pIC50	9	nM	IC50	J Med Chem (2014) 57: 6679-6703 [PMID:24955776]
ChEMBL	Reversible inhibition of human MAOB	B	8.05	pIC50	9	nM	IC50	Bioorg Med Chem Lett (2018) 28: 3596-3600 [PMID:30404719]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI cells using p-tyramine as substrate by fluorometric assay	B	8.1	pIC50	8	nM	IC50	Eur J Med Chem (2020) 207: 112743-112743 [PMID:32882609]
GtoPdb	-	-	8.11	pIC50	7.7	nM	IC50	Bioorg Med Chem (2013) 21: 7435-52 [PMID:24139167]
ChEMBL	Inhibition of human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by fluorescence-based Amplex Red MAO assay	B	8.11	pIC50	7.7	nM	IC50	Bioorg Med Chem Lett (2018) 28: 3596-3600 [PMID:30404719]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus-infected insect cells using p-tyramine as substrate incubated for 30 mins prior to substrate addition measured for 45 mins by amplex red reagent-based microplate fluorescence reader analysis	B	8.12	pIC50	7.67	nM	IC50	J Med Chem (2013) 56: 4580-4596 [PMID:23631427]
ChEMBL	Inhibition of human MAO-B	B	8.12	pIC50	7.67	nM	IC50	Bioorg Med Chem (2013) 21: 7435-7452 [PMID:24139167]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by fluorimetric analysis	B	8.15	pIC50	7	nM	IC50	Eur J Med Chem (2020) 208: 112765-112765 [PMID:32949963]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells assessed as reduction in H2O2 production using p-tyramine as substrate pretreated for 30 mins followed by substrate addition after 30 mins by Amplex red reagent based assay	B	8.22	pIC50	6	nM	IC50	Eur J Med Chem (2017) 130: 365-378 [PMID:28273563]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells assessed as decrease in H2O2 production using p-tyramine as substrate incubated for 20 mins by horse-radish peroxidase/amplex red-based fluorescence method	B	8.29	pIC50	5.18	nM	IC50	Eur J Med Chem (2019) 179: 404-422 [PMID:31265934]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus-infected insect cells using p-tyraimne substrate	B	8.29	pIC50	5.18	nM	IC50	J Med Chem (2014) 57: 6679-6703 [PMID:24955776]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells assessed as decrease in H2O2 production using p-tyramine as substrate incubated for 20 mins by horse-radish peroxidase/amplex red-based fluorescence method	B	8.29	pIC50	5.13	nM	IC50	Eur J Med Chem (2019) 179: 404-422 [PMID:31265934]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by resorufin-based fluorescence assay	B	8.29	pIC50	5.13	nM	IC50	Eur J Med Chem (2019) 162: 793-809 [PMID:30522087]
ChEMBL	Inhibition of human recombinant soluble MAO-B assessed as thermal shift after 20 mins by SYPRO orange staining-based fluorescence assay	B	4.82	pEC50	15000	nM	EC50	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
Monoamine oxidase B in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643]
ChEMBL	Radioenzymatic Assay: The enzyme activity was assessed with a radioenzymatic assay using the substrate 14C-phenylethylamine (PEA) specific for MAO-B.The mitochondrial pellet (500 μg protein) was resuspended in 0.1 M phosphate buffer (pH 7.4). 200 μl of the suspension were added to a 50 μl solution of the test compound or buffer, and incubated for 30 min at 37° C. (preincubation) then the substrate (50 μl) was added. The incubation was carried out for 10 minutes at 37° C. (14C-PEA, 0.5 μM).The reaction was stopped by adding 0.2 ml of perchloric acid. After centrifugation, the deaminated metabolites were extracted with 3 ml of toluene and the radioactive organic phase containing the neutral and/or acidic metabolites formed as a result of MAO-B activity was measured by liquid scintillation spectrometry at 90% efficiency.	B	7	pIC50	100	nM	IC50	US-9051240-B2. Substituted 2-[2-(phenyl) ethylamino] alkaneamide derivatives and their use as sodium and/or calcium channel modulators (2015)
ChEMBL	Inhibition of rat brain mitochondria MAOB by radioenzymatic assay	B	7.01	pIC50	98	nM	IC50	J Med Chem (2007) 50: 4909-4916 [PMID:17824599]
ChEMBL	Inhibition of rat brain MAO-B using [14C]-phenylethylamine substrate after 20 mins	B	7.01	pIC50	98	nM	IC50	J Med Chem (2014) 57: 6679-6703 [PMID:24955776]
ChEMBL	Inhibition of MAO-B in mitochondria-enriched Sprague-Dawley rat liver fractions using p-tyramine as substrate incubated for 30 mins prior to substrate addition measured for 45 mins by amplex red reagent-based microplate fluorescence reader analysis	B	7.59	pIC50	25.8	nM	IC50	J Med Chem (2013) 56: 4580-4596 [PMID:23631427]
ChEMBL	Inhibition of rat recombinant MAO-B expressed in baculovirus-infected insect cells using p-tyraimne substrate	B	7.74	pIC50	18	nM	IC50	J Med Chem (2014) 57: 6679-6703 [PMID:24955776]
Nav1.7/Sodium channel protein type IX alpha subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4296] [GtoPdb: 584] [UniProtKB: Q15858]
ChEMBL	Inhibition of tetrodotoxin-sensitive Na+ channel by patch-clamp electrophysiology	B	4	pIC50	100000	nM	IC50	J Med Chem (2010) 53: 3756-3771 [PMID:20394379]
ChEMBL	Inhibition of tetrodotoxin-sensitive Na+ channel at -50 mV membrane holding potential by patch-clamp electrophysiology	B	4.48	pIC50	33000	nM	IC50	J Med Chem (2010) 53: 3756-3771 [PMID:20394379]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]