salbutamol [Ligand Id: 558] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL714 (Accuneb, Aerolin, Aerolin 400, Aerolin auto, Airomir, Airsalb, Albuterol, Asmasal clickhaler, Asmasal spacehaler, Asmaven, Asmavent, Cobutolin, Combivent, Combivent udvs, Duoneb, Etinoline, Ipramol steri-neb, Kentamol, Libetist, Maxivent, NSC-757417, Proair Hfa, Proventil, Proventil Hfa, PROVENTIL-HFA, Rimasal, Salamol e-breathe, Salapin, Salbulin novolizer, Salbutamol, Salbutamolum, Salbuvent, Salipraneb, SCH 13949W, SCH-13949W, Steri-neb salamol, Ventmax sr, Ventodisks, Ventolin, Ventolin cr, Ventoline, Ventolin e-breathe, Ventolin Hfa, Volmax)
  • β2-adrenoceptor/Beta-2 adrenergic receptor in Human [ChEMBL: CHEMBL210] [GtoPdb: 29] [UniProtKB: P07550]
  • Beta-2 adrenergic receptor in Bovine [ChEMBL: CHEMBL3373] [UniProtKB: Q28044]
  • Beta-2 adrenergic receptor in Guinea pig [ChEMBL: CHEMBL5414] [UniProtKB: Q8K4Z4]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
β2-adrenoceptor/Beta-2 adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL210] [GtoPdb: 29] [UniProtKB: P07550]
ChEMBL Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes assessed as intrinsic Kd by liquid scintillation counting B 5.85 pKd 1400 nM Kd J Med Chem (2016) 59: 5780-5789 [PMID:27239696]
ChEMBL Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes by liquid scintillation counting B 6.66 pKd 220 nM Kd J Med Chem (2016) 59: 5780-5789 [PMID:27239696]
ChEMBL Displacement of [125I]cyanopindolol from human recombinant beta2 adrenergic receptor expressed in CHO cells by filtration assay B 5.74 pKi 1828 nM Ki J. Med. Chem. (2010) 53: 3675-3684 [PMID:20402514]
GtoPdb - - 6.1 pKi 794 nM Ki Mol Pharmacol (1999) 56: 875-85 [PMID:10531390];
Br J Pharmacol (2005) 144: 317-22 [PMID:15655528]
ChEMBL DRUGMATRIX: Adrenergic beta2 radioligand binding (ligand: [3H] CGP-12177) B 6.25 pKi 557 nM Ki DrugMatrix in vitro pharmacology data
ChEMBL Displacement of [3H]CGP1217 from human recombinant adrenergic beta2 receptor transfected in insect Sf9 cells by scintillation counting B 6.29 pKi 510 nM Ki J. Med. Chem. (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL Displacement of [3H]dihydroalprenolol from beta2-adrenoceptor after 90 mins by liquid scintillation counting B 5.7 pIC50 1995.26 nM IC50 Bioorg. Med. Chem. Lett. (2012) 22: 1213-1218 [PMID:22178551]
ChEMBL Displacement of [3H]CGP1217 from human recombinant adrenergic beta2 receptor transfected in insect Sf9 cells by scintillation counting B 6.01 pIC50 980 nM IC50 J. Med. Chem. (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL DRUGMATRIX: Adrenergic beta2 radioligand binding (ligand: [3H] CGP-12177) B 6.09 pIC50 810.2 nM IC50 DrugMatrix in vitro pharmacology data
ChEMBL Agonist activity at human adrenergic beta2 receptor expressed in H292 cells assessed as intracellular cAMP accumulation after 1 hr F 6.7 pEC50 199.53 nM EC50 Bioorg. Med. Chem. Lett. (2011) 21: 4027-4031 [PMID:21652207]
ChEMBL Agonist activity at human beta2 adrenergic receptor assessed as increase in cAMP level by whole cell assay F 7.17 pEC50 68 nM EC50 J. Med. Chem. (2010) 53: 3675-3684 [PMID:20402514]
ChEMBL Agonist activity at recombinant human beta2-adrenoceptor expressed in whole cells assessed as cAMP accumulation by homogeneous radioimmunoassay F 7.6 pEC50 25.12 nM EC50 Bioorg. Med. Chem. Lett. (2012) 22: 1213-1218 [PMID:22178551]
Beta-2 adrenergic receptor in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3373] [UniProtKB: Q28044]
ChEMBL Apparent binding affinity constant to beta-2 adrenergic receptor determined using [3H]DHA B 6.24 pKd 0.57 uM KD app J. Med. Chem. (1986) 29: 549-554 [PMID:2870189]
ChEMBL Affinity for cow beta-2 adrenergic receptor by measuring displacement (-)-[3H]dihydroalprenolol (DHA) B 6.28 pKd 520 nM Kd J. Med. Chem. (1985) 28: 1328-1334 [PMID:2993621]
ChEMBL Affinity for cow beta-2 adrenergic receptor by measuring displacement (-)-[3H]dihydroalprenolol (DHA) B 6.58 pKd 6.58 - -Log KD J. Med. Chem. (1985) 28: 1328-1334 [PMID:2993621]
Beta-2 adrenergic receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5414] [UniProtKB: Q8K4Z4]
ChEMBL Binding affinity to adrenergic beta2 receptor in Hartley guinea pig tracheal ring B 5.94 pKd 1160 nM Kd Eur. J. Med. Chem. (2009) 44: 2840-2846 [PMID:19168263]
ChEMBL Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against histamine-induced contraction B 6.61 pKd 245.47 nM Kd Bioorg. Med. Chem. Lett. (2015) 25: 820-825 [PMID:25592716]
ChEMBL Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring at basal tone assessed as bronchorelaxation activity B 6.96 pKd 109.65 nM Kd Bioorg. Med. Chem. Lett. (2015) 25: 820-825 [PMID:25592716]
ChEMBL Agonist activity at beta2 adrenergic receptor in guinea pig tracheal strip assessed as inhibition of electrically-induced bronchocontractile response after 30 mins F 7.77 pIC50 17 nM IC50 J. Med. Chem. (2010) 53: 3675-3684 [PMID:20402514]
ChEMBL Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against carbachol-induced contraction F 6.57 pEC50 268 nM EC50 Eur. J. Med. Chem. (2009) 44: 2840-2846 [PMID:19168263]
ChEMBL Agonist activity at adrenergic beta2 receptor in guinea pig trachea assessed as relaxation of carbachol-induced tissue contraction F 8.04 pEC50 9.1 nM EC50 J. Med. Chem. (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against histamine-induced contraction F 9.34 pEC50 0.45 nM EC50 Eur. J. Med. Chem. (2009) 44: 2840-2846 [PMID:19168263]
GroEL/GroES in Escherichia coli (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1Q7BGE6]
ChEMBL Inhibition of Escherichia coli GroEL expressed in Escherichia coliDH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured soluble pig heart MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 20 to 40 mins by malachite green dye based spectrometric analysis B 4 pIC50 >100000 nM IC50 Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]
HSP60/HSP10 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106131] [UniProtKB: P10809P61604]
ChEMBL Inhibition of human N-terminal octa-His-tagged HSP60 expressed in Escherichia coli Rosetta(DE3) pLysS/human HSP10 expressed in Escherichia coli Rosetta(DE3) assessed as reduction in HSP60/HSP10-mediated denatured MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 40 to 60 mins by malachite green dye based spectrometric analysis B 4 pIC50 >100000 nM IC50 Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]

ChEMBL data shown on this page come from version 28:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]