ChEMBL ligand: CHEMBL295467 (COMPOUND 89218, COMPOUND-89218, LY-94939, Nisoxetina, Nisoxetine, NSC-283481)
5-HT1A receptor/5-hydroxytryptamine receptor 1A in Rat [ChEMBL: CHEMBL273] [GtoPdb: 1] [UniProtKB: P19327] There should be some charts here, you may need to enable JavaScript!
5-HT2A receptor/5-hydroxytryptamine receptor 2A in Rat [ChEMBL: CHEMBL322] [GtoPdb: 6] [UniProtKB: P14842] There should be some charts here, you may need to enable JavaScript!
Norepinephrine transporter in Rat [ChEMBL: CHEMBL304] [UniProtKB: Q9WTR4] There should be some charts here, you may need to enable JavaScript!
DAT/Sodium-dependent dopamine transporter in Human [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959] DAT/Sodium-dependent dopamine transporter in Rat [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977] There should be some charts here, you may need to enable JavaScript!
NET/Sodium-dependent noradrenaline transporter in Human [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975] There should be some charts here, you may need to enable JavaScript!
SERT/Sodium-dependent serotonin transporter in Human [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645] SERT/Sodium-dependent serotonin transporter in Rat [ChEMBL: CHEMBL313] [GtoPdb: 928] [UniProtKB: P31652] There should be some charts here, you may need to enable JavaScript!
Transporter in Rat [ChEMBL: CHEMBL6184] [UniProtKB: Q63380] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
5-HT1A receptor/5-hydroxytryptamine receptor 1A in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL273] [GtoPdb: 1] [UniProtKB: P19327]
ChEMBL	Binding affinity against 5-hydroxytryptamine 1A receptor (5-HT1A) by displacement of [3H]8-OH-DPAT from rat hippocampus membranes	B	6	pIC50	>1000	nM	IC50	J Med Chem (2003) 46: 5512-5532 [PMID:14640559]
5-HT2A receptor/5-hydroxytryptamine receptor 2A in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL322] [GtoPdb: 6] [UniProtKB: P14842]
ChEMBL	Binding affinity against 5-hydroxytryptamine 2A receptor by displacement of [3H]-ketanserin from rat prefrontal cerebral cortex mambranes	B	6	pIC50	>1000	nM	IC50	J Med Chem (2003) 46: 5512-5532 [PMID:14640559]
Norepinephrine transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL304] [UniProtKB: Q9WTR4]
ChEMBL	Binding affinity against norepinephrine transporter (NET) by displacement of [3H]nisoxetine in male wistar rats	B	8	pKi	9.9	nM	Ki	J Med Chem (2003) 46: 5512-5532 [PMID:14640559]
ChEMBL	Displacement of [3H]-nisoxetine from NET in Sprague-Dawley rat brain prefrontal cortex incubated for 180 mins by radioligand binding assay	B	8.82	pKi	1.5	nM	Ki	RSC Med Chem (2021) 12: 1174-1186 [PMID:34355183]
DAT/Sodium-dependent dopamine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959]
ChEMBL	Binding affinity for dopamine transporter expressed in LCK PK1 cells	B	6	pKi	>1000	nM	Ki	J Med Chem (2004) 47: 5258-5264 [PMID:15456270]
ChEMBL	Uptake Assay Protocol: DAT: This protocol was designed to measure inhibition of uptake by the human dopamine transporter. The reagents were human DAT (HEK293F) cells, GBR 12909 (Sigma), nomifensine, neurotransmitter transporter uptake assay kit (Molecular Devices), freestyle 293 expression medium (Invitrogen), 10× Hank's Balanced Salt Solution (HBSS; Invitrogen), 1 M HEPES (Mediatech), Biocoat poly-D-lysine 96-well, black, clear plates (Becton, Dickinson), and 500 pt polypropylene U-bottom 96-well plates (Fisher). The Assay Buffer (AB) was 1×HBSS and 0.02 M HEPES.The HEK293F cells were transfected with the human dopamine transporter and frozen in 1 mL aliquots at about 1E+07 cells/mL. On the day of the experiment, the cells were removed from −80° C. or liquid nitrogen and thawed in a room temperature water bath. The cells were dilute to about 1-2E+06 with Freestyle medium. A 1 mL sample (1:2 dilution) was prepared, and the cells were counted. The cells were spun at 1100 rpm for 5 minutes, and the medium was aspirated off. The cells were resuspend in medium at 1.5E+06 cells/mL for about 60,000 cells per well. 40 μL of cells were dispensed per well in the Biocoat plates. The plates were spun at 1100 rpm for 1 minute to improve homogeneity of the cell layer and were incubated at 37° C. for a minimum of 3 hours.12 μL of test compound (10 mM) in DMSO was added to the wells, and nomifensine was used as a control. GBR 12909 (final assay concentration of 10 μM) was used for background signal. The neurotransmitter transporter dye was prepared in AB prior to use.	B	7.08	pKi	82.5	nM	Ki	US-9944618-B2. Inhibiting neurotransmitter reuptake (2018)
ChEMBL	In vitro competitive binding versus [N-methyl-3H]WIN-35428 in murine kidney cells transfected with cDNA for human dopamine transporter (DAT)	B	7.52	pKi	30	nM	Ki	J Med Chem (2003) 46: 925-935 [PMID:12620070]
ChEMBL	Inhibition of dopamine uptake at human DAT expressed in HEK cells	B	5.62	pIC50	2400	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4495-4498 [PMID:18667309]
ChEMBL	Inhibition of dopamine uptake at human DAT in human HEK293 cells	B	5.62	pIC50	2400	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4224-4227 [PMID:18550369]
ChEMBL	Inhibition of human DAT expressed in HEK293 cells assessed as inhibition of dopamine reuptake after 30 mins by fluorescence assay	B	5.94	pIC50	1150	nM	IC50	J Med Chem (2012) 55: 8188-8192 [PMID:22938049]
ChEMBL	Inhibition of DA reuptake at human DAT expressed in HEK293 cells after 15 mins by fluorescence neurotransmitter transporter assay	B	5.94	pIC50	1150	nM	IC50	Bioorg Med Chem Lett (2014) 24: 3234-3237 [PMID:24974340]
ChEMBL	Inhibition of human DAT expressed in HEK293 cells at incubated for 15 mins by neurotransmitter reuptake assay	B	5.94	pIC50	1150	nM	IC50	ACS Med Chem Lett (2014) 5: 999-1004 [PMID:25221656]
DAT/Sodium-dependent dopamine transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977]
ChEMBL	Binding affinity against dopamine transporter (DAT) by displacement of [3H]WIN-35428 in male wistar rats	B	6.3	pIC50	>500	nM	IC50	J Med Chem (2003) 46: 5512-5532 [PMID:14640559]
NET/Sodium-dependent noradrenaline transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975]
ChEMBL	Inhibition of norepinephrine reuptake at NET	B	8.22	pKi	6	nM	Ki	Bioorg Med Chem (2009) 17: 7802-7815 [PMID:19836247]
GtoPdb	-	-	8.4	pKi	3.98	nM	Ki
ChEMBL	Binding affinity for Norepinephrine transporter (NET) expressed in LCK PK1 cells	B	8.8	pKi	1.6	nM	Ki	J Med Chem (2004) 47: 5258-5264 [PMID:15456270]
ChEMBL	Uptake Assay Protocol: NET: This protocol was designed to measure inhibition of uptake by the human norepinephrine transporter. The reagents were human NET (HEK293F) cells, desipramine (Sigma), nomifensine, neurotransmitter transporter uptake assay kit (Molecular Devices), freestyle 293 expression medium (Invitrogen), 10× Hank's Balanced Salt Solution (HBSS; Invitrogen), 1 M HEPES (Mediatech), Biocoat poly-D-lysine 96-well, black, clear plates (Becton, Dickinson), and 500 μL polypropylene U-bottom 96-well plates (Fisher). The Assay Buffer (AB) was 1×HBSS and 0.02 M HEPES.The HEK293F cells were transfected with the human norepinephrine transporter and frozen in 1 mL aliquots at about 1E+07 cells/mL. On the day of the experiment, the cells were removed from −80° C. or liquid nitrogen and thawed in a room temperature water bath. The cells were dilute to about 1-2E+06 with Freestyle medium. A 1 mL sample (1:2 dilution) was prepared, and the cells were counted. The cells were spun at 1100 rpm for 5 minutes, and the medium was aspirated off. The cells were resuspend in medium at 1.5E+06 cells/mL for about 60,000 cells per well. 40 μL of cells were dispensed per well in the Biocoat plates. The plates were spun at 1100 rpm for 1 minute to improve homogeneity of the cell layer and were incubated at 37° C. for a minimum of 3 hours.	B	8.89	pKi	1.3	nM	Ki	US-9944618-B2. Inhibiting neurotransmitter reuptake (2018)
ChEMBL	Inhibition of norepinephrine reuptake at human NET expressed in HEK293 cells after 15 mins by fluorescence neurotransmitter transporter assay	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2014) 24: 3234-3237 [PMID:24974340]
ChEMBL	Inhibition of human NET expressed in HEK293 cells at incubated for 15 mins by neurotransmitter reuptake assay	B	7.7	pIC50	20	nM	IC50	ACS Med Chem Lett (2014) 5: 999-1004 [PMID:25221656]
ChEMBL	Inhibition of human NET expressed in HEK293 cells assessed as inhibition of noradrenaline reuptake after 30 mins by fluorescence assay	B	7.74	pIC50	18	nM	IC50	J Med Chem (2012) 55: 8188-8192 [PMID:22938049]
ChEMBL	Inhibition of norepinephrine uptake at human NET expressed in HEK cells	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4495-4498 [PMID:18667309]
ChEMBL	Inhibition of norepinephrine uptake at human NET in human HEK293 cells	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4224-4227 [PMID:18550369]
ChEMBL	Inhibition of [3H]norepinephrine reuptake at human NET expressed in MDCK cells by scintillation counting	B	8.08	pIC50	8.3	nM	IC50	Bioorg Med Chem (2011) 19: 663-676 [PMID:21093273]
ChEMBL	Inhibition of norepinephrine reuptake at NET expressed in MDCK cells	B	8.1	pIC50	7.94	nM	IC50	J Med Chem (2011) 54: 5283-5295 [PMID:21739935]
ChEMBL	Inhibition of [3H]norepinephrine reuptake at human recombinant NET expressed in HEK293 cells by scintillation counting	B	8.1	pIC50	7.94	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1434-1437 [PMID:21310612]
ChEMBL	Inhibition of norepinephrine uptake at human NET expressed in MDCK cells	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4929-4931 [PMID:18771916]
ChEMBL	Inhibition of human NET expressed in COS7 cells assessed as inhibition of [3H]norepinephrine uptake	B	8.99	pEC50	-8.99	-	logEC50	J Med Chem (2009) 52: 6991-7002 [PMID:19860431]
SERT/Sodium-dependent serotonin transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645]
ChEMBL	Binding affinity for serotonin transporter expressed in LCK PK1 cells	B	6.69	pKi	203	nM	Ki	J Med Chem (2004) 47: 5258-5264 [PMID:15456270]
ChEMBL	Uptake Assay Protocol: SERT: This protocol was designed to measure inhibition of uptake by the human serotonin transporter. The reagents were human SERT (HEK293F) cells, fluoxetine (Sigma), nomifensine, neurotransmitter transporter uptake assay kit (Molecular Devices), freestyle 293 expression medium (Invitrogen), 10× Hank's Balanced Salt Solution (HBSS; Invitrogen), 1 M HEPES (Mediatech), Biocoat poly-D-lysine 96-well, black, clear plates (Becton, Dickinson), and 500 μL polypropylene U-bottom 96-well plates (Fisher). The Assay Buffer (AB) was 1λ HBSS and 0.02 M HEPES.The HEK293F cells were transfected with the human serotonin transporter and frozen in 1 mL aliquots at about 1E+07 cells/mL. On the day of the experiment, the cells were removed from −80° C. or liquid nitrogen and thawed in a room temperature water bath. The cells were dilute to about 1-2E+06 with Freestyle medium. A 1 mL sample (1:2 dilution) was prepared, and the cells were counted. The cells were spun at 1100 rpm for 5 minutes, and the medium was aspirated off. The cells were resuspend in medium at 1.5E+06 cells/mL for about 60,000 cells per well. 40 μL of cells were dispensed per well in the Biocoat plates. The plates were spun at 1100 rpm for 1 minute to improve homogeneity of the cell layer and were incubated at 37° C. for a minimum of 3 hours.	B	7.26	pKi	54.4	nM	Ki	US-9944618-B2. Inhibiting neurotransmitter reuptake (2018)
ChEMBL	In vitro competitive binding versus [3H]-citalopram in murine kidney cells transfected with cDNA for human serotonin transporter (SERT)	B	8.85	pKi	1.4	nM	Ki	J Med Chem (2003) 46: 925-935 [PMID:12620070]
ChEMBL	Inhibition of serotonin uptake at human SERT expressed in HEK cells	B	5.85	pIC50	1400	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4495-4498 [PMID:18667309]
ChEMBL	Inhibition of serotonin uptake at human SERT in human HEK293 cells	B	5.85	pIC50	1400	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4224-4227 [PMID:18550369]
ChEMBL	Inhibition of human SERT expressed in HEK293 cells assessed as inhibition of serotonin reuptake after 30 mins by fluorescence assay	B	6.15	pIC50	700	nM	IC50	J Med Chem (2012) 55: 8188-8192 [PMID:22938049]
ChEMBL	Inhibition of serotonin reuptake at human SERT expressed in HEK293 cells after 15 mins by fluorescence neurotransmitter transporter assay	B	6.15	pIC50	700	nM	IC50	Bioorg Med Chem Lett (2014) 24: 3234-3237 [PMID:24974340]
ChEMBL	Inhibition of human SERT expressed in HEK293 cells at incubated for 15 mins by neurotransmitter reuptake assay	B	6.15	pIC50	700	nM	IC50	ACS Med Chem Lett (2014) 5: 999-1004 [PMID:25221656]
ChEMBL	Inhibition of serotonin uptake at human SERT expressed in JAR cells	B	6.56	pIC50	277	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4929-4931 [PMID:18771916]
SERT/Sodium-dependent serotonin transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL313] [GtoPdb: 928] [UniProtKB: P31652]
ChEMBL	Displacement of [3H]citalopram from SERT in Sprague-dawley rat frontal cortex by liquid scintillation spectrophotometry	B	6.56	pKi	277	nM	Ki	Bioorg Med Chem Lett (2009) 19: 58-61 [PMID:19038547]
ChEMBL	Binding affinity against serotonin transporter (SERT) by displacement of [3H]paroxetine in male wistar rats	B	6.3	pIC50	>500	nM	IC50	J Med Chem (2003) 46: 5512-5532 [PMID:14640559]
Transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6184] [UniProtKB: Q63380]
ChEMBL	Displacement of [3H]nisoxetine from Sprague-dawley rat NET by liquid scintillation spectrophotometry	B	8.22	pKi	6	nM	Ki	Bioorg Med Chem Lett (2009) 19: 58-61 [PMID:19038547]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]