β-phenylethylamine [Ligand Id: 2144] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL610 (Phenethylamine, Phenylethylamine)
There should be some charts here, you may need to enable JavaScript!
  • 5-HT2A receptor/Serotonin 2a (5-HT2a) receptor in Human [ChEMBL: CHEMBL224] [GtoPdb: 6] [UniProtKB: P28223]
  • 5-HT2A receptor/Serotonin 2a (5-HT2a) receptor in Rat [ChEMBL: CHEMBL322] [GtoPdb: 6] [UniProtKB: P14842]
There should be some charts here, you may need to enable JavaScript!
  • TA1 receptor/Trace amine-associated receptor 1 in Human [ChEMBL: CHEMBL5857] [GtoPdb: 364] [UniProtKB: Q96RJ0]
  • TA1 receptor/Trace amine-associated receptor 1 in Mouse [ChEMBL: CHEMBL4908] [GtoPdb: 364] [UniProtKB: Q923Y8]
  • TA1 receptor/Trace amine-associated receptor 1 in Rat [ChEMBL: CHEMBL3833] [GtoPdb: 364] [UniProtKB: Q923Y9]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • TAAR4P in Mouse [GtoPdb: 169] [UniProtKB: Q5QD15]
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
lysyl oxidase like 2/Lysyl oxidase homolog 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3714029] [GtoPdb: 2853] [UniProtKB: Q9Y4K0]
ChEMBL Inhibition of full length recombinant human LOXL2 expressed in CHO cells assessed as reduction in H2O2 production using DAP as substrate preincubated for 2 hrs followed by substrate addition measured every 2 minutes for 50 minutes by Amplex red dye based fluorescence assay B 4.22 pIC50 60100 nM IC50 ACS Med Chem Lett (2017) 8: 423-427 [PMID:28435530]
5-HT2A receptor/Serotonin 2a (5-HT2a) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL224] [GtoPdb: 6] [UniProtKB: P28223]
ChEMBL Binding affinity was performed using [3H]ketanserin as the radioligand and stably transfected NIH3T3 cells expressing the 5-hydroxytryptamine 2A receptor (GF-62 cells). B 4.77 pKi 16800 nM Ki J Med Chem (2002) 45: 1656-1664 [PMID:11931619]
ChEMBL Displacement of [3H]ketanserin from 5HT2A receptor expressed in NIH3T3 cells B 4.77 pKi 16800 nM Ki J Med Chem (2008) 51: 6808-6828 [PMID:18847250]
5-HT2A receptor/Serotonin 2a (5-HT2a) receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL322] [GtoPdb: 6] [UniProtKB: P14842]
ChEMBL Binding affinity against 5-hydroxytryptamine 2A receptor expressed NIH3T3 cells using [3H]ketanserin B 4.77 pKi 16820 nM Ki Bioorg Med Chem Lett (2001) 11: 563-566 [PMID:11229772]
ChEMBL Displacement of [3H]ketanserin from rat 5HT2A expressed in mouse NIH/3T3 cell membranes after 30 mins by scintillation counting method B 5 pKi >10000 nM Ki J Med Chem (2017) 60: 2605-2628 [PMID:28244748]
TA1 receptor/Trace amine-associated receptor 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5857] [GtoPdb: 364] [UniProtKB: Q96RJ0]
ChEMBL Agonist activity at human TAAR1 expressed in HEK293 cells assessed as cAMP accumulation after 20 mins by BRET assay F 6.89 pEC50 130 nM EC50 Eur J Med Chem (2018) 146: 171-184 [PMID:29407948]
ChEMBL Agonist activity at human trace amine associated receptor 1 expressed in RD-HGA16 CHO-K1 cells coexpressed with Galpha16 protein assessed as internal calcium mobilization by calcium 3 assay F 6.89 pEC50 129 nM EC50 Bioorg Med Chem (2008) 16: 7415-7423 [PMID:18602830]
ChEMBL Agonist activity at human TAAR1 receptor expressed in HEK293T cells cotransfected with beta-2 receptor assessed as cAMP production by BRET assay F 6.96 pEC50 110 nM EC50 Medchemcomm (2015) 6: 2216-2223
ChEMBL Activation of human TAAR1 expressed in Syrian hamster AV12-664 cells co-expressing GalphaS protein assessed as accumulation of cAMP after 30 mins using cAMP XS EA/CL as substrate by luminescence method in presence of RX821002 and alprenolol F 6.97 pEC50 106 nM EC50 Bioorg Med Chem (2011) 19: 7044-7048 [PMID:22037049]
GtoPdb - - 7 pEC50 - - - Proc Natl Acad Sci USA (2001) 98: 8966-71 [PMID:11459929];
Mol Pharmacol (2008) 74: 585-594 [PMID:18524885];
J Pharmacol Exp Ther (2007) 320: 475-85 [PMID:17038507];
Bioorg Med Chem (2008) 16: 7415-23 [PMID:18602830];
Biochem J (2009) 424: 39-45 [PMID:19725810];
PLoS ONE (2011) 6: e27073 [PMID:22073124]
ChEMBL Agonist activity at recombinant human TAAR1 expressed in CHO-K1 cells assessed as increase in intracellular cAMP incubated for 30 mins by HTRF analysis F 7.82 pEC50 15 nM EC50 ACS Med Chem Lett (2022) 13: 92-98 [PMID:35047111]
TA1 receptor/Trace amine-associated receptor 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4908] [GtoPdb: 364] [UniProtKB: Q923Y8]
ChEMBL Activation of mouse wild type TAAR1 expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay F 6.72 pEC50 189 nM EC50 J Med Chem (2014) 57: 378-390 [PMID:24354319]
ChEMBL Agonist activity at mouse TAAR1 expressed in HEK293 cells coexpressing cAMP BRET biosensor assessed as induction of CAMP production after 20 mins by bioluminescence resonance energy transfer assay F 6.86 pEC50 138 nM EC50 J Med Chem (2015) 58: 5096-5107 [PMID:26010728]
ChEMBL Agonist activity at mouse TAAR1 expressed in HEK293T cells assessed as induction of cAMP production by BRET assay F 6.86 pEC50 138 nM EC50 J Med Chem (2016) 59: 9825-9836 [PMID:27731647]
ChEMBL Agonist activity at mouse TAAR1 expressed in HEK293 cells assessed as cAMP accumulation after 20 mins by BRET assay F 6.92 pEC50 120 nM EC50 Eur J Med Chem (2018) 146: 171-184 [PMID:29407948]
ChEMBL Activation of mouse TAAR1 transmembrane domain 7 Tyr287(7.39)Asn mutant expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay F 7.23 pEC50 58.6 nM EC50 J Med Chem (2014) 57: 378-390 [PMID:24354319]
ChEMBL Activation of mouse TAAR1 transmembrane domain 6 Thr268(6.55)Met mutant expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay F 7.33 pEC50 47.3 nM EC50 J Med Chem (2014) 57: 378-390 [PMID:24354319]
GtoPdb - - 7.4 pEC50 - - - J Pharmacol Exp Ther (2007) 321: 178-86 [PMID:17218486];
Genes Brain Behav (2007) 6: 628-39 [PMID:17212650];
Biochem J (2009) 424: 39-45 [PMID:19725810]
TA1 receptor/Trace amine-associated receptor 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3833] [GtoPdb: 364] [UniProtKB: Q923Y9]
ChEMBL Activation of rat TAAR1 transmembrane domain 6 Met268(6.55)Thr mutant expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay F 6.58 pEC50 261.2 nM EC50 J Med Chem (2014) 57: 378-390 [PMID:24354319]
GtoPdb - - 6.6 pEC50 - - - J Pharmacol Exp Ther (2007) 321: 178-86 [PMID:17218486];
Mol Pharmacol (2001) 60: 1181-8 [PMID:11723224]
ChEMBL Agonist activity at rat N-terminal FLAG-tagged TAAR1 expressed in HEK293 cells assessed as [3H]cAMP accumulation measured after 1 hr F 6.62 pEC50 240 nM EC50 J Med Chem (2017) 60: 2605-2628 [PMID:28244748]
ChEMBL Activation of rat TAAR1 expressed in Syrian hamster AV12-664 cells assessed as accumulation of cAMP after 30 mins using cAMP XS EA/CL as substrate by luminescence method F 6.68 pEC50 209 nM EC50 Bioorg Med Chem (2011) 19: 7044-7048 [PMID:22037049]
ChEMBL Activation of rat TAAR1 transmembrane domain 7 Asn287(7.39)Tyr mutant expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay F 6.77 pEC50 170.6 nM EC50 J Med Chem (2014) 57: 378-390 [PMID:24354319]
ChEMBL Activation of rat wild type TAAR1 expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay F 7.39 pEC50 40.33 nM EC50 J Med Chem (2014) 57: 378-390 [PMID:24354319]
serine protease 1/Trypsin I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL209] [GtoPdb: 2397] [UniProtKB: P07477]
ChEMBL Binding affinity against trypsin B 1.96 pKd -1.96 - Log Kd J Med Chem (2002) 45: 2770-2780 [PMID:12061879]
Trypsin I in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3769] [UniProtKB: P00760]
ChEMBL Binding affinity against bovine trypsin B 4.96 pKi 11000 nM Ki J Med Chem (2002) 45: 2469-2483 [PMID:12036355]
TAAR4P in Mouse [GtoPdb: 169] [UniProtKB: Q5QD15]
GtoPdb - - 6 pEC50 1000 nM EC50 Nature (2006) 442: 645-650 [PMID:16878137]

ChEMBL data shown on this page come from version 32:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]