ChEMBL ligand: CHEMBL395280 ((-)-huperzine a, Huperzine a, L-huperzine a, (-)-selagine, Selagine)
Acetylcholinesterase in Torpedo californica [ChEMBL: CHEMBL4780] [UniProtKB: P04058] Acetylcholinesterase in Electrophorus electricus [ChEMBL: CHEMBL4078] [UniProtKB: O42275] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303] Acetylcholinesterase in Bovine [ChEMBL: CHEMBL4768] [UniProtKB: P23795] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Mouse [ChEMBL: CHEMBL3198] [GtoPdb: 2465] [UniProtKB: P21836] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Rat [ChEMBL: CHEMBL3199] [GtoPdb: 2465] [UniProtKB: P37136] There should be some charts here, you may need to enable JavaScript!
Butyrylcholinesterase in Rat [ChEMBL: CHEMBL3403] [UniProtKB: Q9JKC1] There should be some charts here, you may need to enable JavaScript!
butyrylcholinesterase/Cholinesterase in Human [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Acetylcholinesterase in Torpedo californica (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4780] [UniProtKB: P04058]
ChEMBL	Inhibition of Torpedo californica AChE	B	6.76	pKi	175	nM	Ki	J Med Chem (2008) 51: 3154-3170 [PMID:18479118]
ChEMBL	Inhibition of Torpedo californica AChE	B	6.76	pKi	175	nM	Ki	J Med Chem (2008) 51: 3154-3170 [PMID:18479118]
ChEMBL	Inhibition of Torpedo california AChE by Ellman's method	B	7.94	pIC50	11.4	nM	IC50	Bioorg Med Chem (2009) 17: 6937-6941 [PMID:19726199]
Acetylcholinesterase in Electrophorus electricus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4078] [UniProtKB: O42275]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as a substrate incubated for 20 mins followed by substrate addition and measured after 20 mins by Ellman's method	B	4.96	pIC50	11000	nM	IC50	J Nat Prod (2021) 84: 2374-2379 [PMID:34445873]
ChEMBL	Inhibition of Electric eel AChE using acetylcholine iodide as substrate by spectrophotometry based Ellman's method	B	5.29	pIC50	5170	nM	IC50	Bioorg Med Chem Lett (2020) 30: 126985-126985 [PMID:32008906]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate after 15 mins by Ellman's method	B	6.92	pIC50	120	nM	IC50	J Nat Prod (2011) 74: 997-1002 [PMID:21510637]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303]
ChEMBL	Binding affinity to CM5 sensor chip immobilized recombinant human AChE assessed as dissociation constant at 298.15 K by surface plasmon resonance assay	B	7.77	pKd	17	nM	Kd	J Med Chem (2021) 64: 1844-1855 [PMID:33570950]
GtoPdb	-	-	7.77	pKd	17	nM	Kd	J Med Chem (2021) 64: 1844-1855 [PMID:33570950]
ChEMBL	Inhibition of human AChE	B	7.33	pKi	47	nM	Ki	J Med Chem (2006) 49: 3421-3425 [PMID:16722663]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate by DTNB reagent based Ellman's method	B	5.28	pIC50	5280	nM	IC50	Bioorg Med Chem Lett (2022) 72: 128873-128873 [PMID:35779827]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate after 5 mins by spectrophotometric analysis	B	5.37	pIC50	4300	nM	IC50	J Nat Prod (2019) 82: 792-797 [PMID:30794407]
ChEMBL	Inhibition of AChE by Ellman's method	B	5.77	pIC50	1700	nM	IC50	J Nat Prod (2010) 73: 1858-1862 [PMID:20954721]
ChEMBL	Inhibition of human acetylcholinesterase after 30 mins by microplate reader-based Ellman's method	B	6.22	pIC50	600	nM	IC50	J Nat Prod (2015) 78: 3067-3070 [PMID:26618211]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by Ellman's method	B	6.4	pIC50	400	nM	IC50	J Nat Prod (2018) 81: 2115-2119 [PMID:30183289]
ChEMBL	Inhibitory concentration against Acetylcholinesterase from human erythrocytes	B	6.59	pIC50	260	nM	IC50	J Med Chem (2005) 48: 1701-1704 [PMID:15771413]
ChEMBL	In vitro inhibitory concentration against acetylcholinesterase of human erythrocytes	B	6.59	pIC50	260	nM	IC50	J Med Chem (2001) 44: 4733-4736 [PMID:11741490]
ChEMBL	Inhibition of human erythrocyte AChE	B	6.59	pIC50	260	nM	IC50	J Med Chem (2006) 49: 6833-6840 [PMID:17154513]
ChEMBL	Inhibition of acetylcholinesterase isolated from Human erythrocytes.	B	6.59	pIC50	260	nM	IC50	J Med Chem (2000) 43: 4657-4666 [PMID:11101357]
ChEMBL	Inhibitory activity against acetylcholinesterase in human RBC	B	6.82	pIC50	150	nM	IC50	Bioorg Med Chem Lett (1996) 6: 737-742
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate after 8 mins by Ellman's spectrophotometric method	B	6.9	pIC50	125	nM	IC50	J Nat Prod (2013) 76: 1475-1484 [PMID:23941108]
ChEMBL	Ex vivo inhibition of human erythrocyte Acetylcholinesterase.	B	7.33	pIC50	47	nM	IC50	J Med Chem (2002) 45: 3684-3691 [PMID:12166941]
ChEMBL	Inhibitory concentration against human erythrocyte Acetylcholinesterase	B	7.33	pIC50	47	nM	IC50	J Med Chem (2005) 48: 986-994 [PMID:15715468]
ChEMBL	Anticholinesterase activity against human erythrocyte AChE	B	7.33	pIC50	47	nM	IC50	J Med Chem (2006) 49: 2174-2185 [PMID:16570913]
ChEMBL	Inhibition of AChE (unknown origin)	B	7.35	pIC50	45	nM	IC50	Bioorg Med Chem Lett (2013) 23: 1544-1547 [PMID:23395652]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate measured for 1 min by Ellman's method	B	7.48	pIC50	33	nM	IC50	J Nat Prod (2019) 82: 239-248 [PMID:30701972]
ChEMBL	DRUGMATRIX: Acetylcholinesterase enzyme inhibition (substrate: acetylthiocholine)	B	7.54	pIC50	29	nM	IC50	DrugMatrix in vitro pharmacology data
ChEMBL	Inhibition of human AChE by Ellman's method	B	7.68	pIC50	21	nM	IC50	Bioorg Med Chem (2009) 17: 6937-6941 [PMID:19726199]
ChEMBL	Inhibition of human AChE pre-incubated for 10 mins before acetylthiocholine iodide substrate addition	B	7.92	pIC50	12	nM	IC50	J Nat Prod (2012) 75: 1400-1404 [PMID:22775474]
ChEMBL	Inhibition of AChE-induced amyloid beta aggregation	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2687-2691 [PMID:21216144]
ChEMBL	In vitro inhibitory activity against Acetylcholinesterase (AChE).	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (1996) 6: 1927-1930
ChEMBL	Inhibition of AChE (unknown origin)	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4848-4853 [PMID:26159481]
ChEMBL	Inhibition of acetylcholinesterase (unknown origin) assessed as inhibition of acetylcholine hydrolysis after 30 mins by Ellmann's method	B	9.27	pIC50	0.54	nM	IC50	J Nat Prod (2014) 77: 2054-2059 [PMID:25222040]
Acetylcholinesterase in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4768] [UniProtKB: P23795]
ChEMBL	Inhibitory constant against fetal bovine serum (FBS) acetylcholinesterase	B	7.62	pKi	24	nM	Ki	Bioorg Med Chem Lett (2002) 12: 1521-1523 [PMID:12031333]
ChEMBL	Dissociation constant for the inhibition of fetal bovine serum acetylcholinesterase was determined..	B	7.63	pKi	23.6	nM	Ki	Bioorg Med Chem Lett (1996) 6: 259-262
ChEMBL	Dissociation constant for the inhibition of fetal bovine serum acetylcholinesterase was determined..	B	8.23	pKi	5.9	nM	Ki	Bioorg Med Chem Lett (1996) 6: 259-262
ChEMBL	Binding affinity towards Acetylcholinesterase from fetal bovine serum	B	8.23	pKi	5.9	nM	Ki	Bioorg Med Chem Lett (2000) 10: 2467-2469 [PMID:11078202]
ChEMBL	Inhibition of bovine erythrocytes AChE using acetylthiocholine iodide as substrate incubated for 15 to 20 mins by Ellman's methods	B	7	pIC50	100	nM	IC50	J Nat Prod (2020) 83: 2831-2843 [PMID:32941036]
ChEMBL	Compound was evaluated for the inhibition of acetylcholinesterase of bovine erythrocytes	B	7.13	pIC50	74	nM	IC50	J Med Chem (2000) 43: 4657-4666 [PMID:11101357]
ChEMBL	In vitro inhibitory concentration against bovine acetylcholinesterase enzyme	B	7.13	pIC50	74	nM	IC50	J Med Chem (2001) 44: 4733-4736 [PMID:11741490]
ChEMBL	Inhibitory concentration against Acetylcholinesterase from bovine erythrocytes	B	7.13	pIC50	74	nM	IC50	J Med Chem (2005) 48: 1701-1704 [PMID:15771413]
ChEMBL	Inhibition of acetylcholinesterase (AChE)activity in bovine erythrocytes	B	7.13	pIC50	74	nM	IC50	J Med Chem (1999) 42: 3227-3242 [PMID:10464010]
ChEMBL	Inhibition of bovine AChE by Ellman's method	B	7.13	pIC50	74	nM	IC50	Bioorg Med Chem (2008) 16: 7450-7456 [PMID:18585045]
ChEMBL	Inhibition of bovine erythrocyte AChE	B	7.13	pIC50	74	nM	IC50	J Med Chem (2006) 49: 6833-6840 [PMID:17154513]
ChEMBL	Inhibition of bovine acetylcholinesterase	B	7.28	pIC50	53	nM	IC50	Bioorg Med Chem (2007) 15: 7803-7808 [PMID:17904850]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3198] [GtoPdb: 2465] [UniProtKB: P21836]
ChEMBL	Inhibition of mouse AChE	B	7.09	pIC50	82	nM	IC50	J Med Chem (2013) 56: 7615-7624 [PMID:23984975]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3199] [GtoPdb: 2465] [UniProtKB: P37136]
ChEMBL	Compound was tested for its ability to inhibit acetylcholinesterase (AChE) in rat brain; 0.024-0.040 uM	B	7.62	pKi	24	nM	Ki	Bioorg Med Chem Lett (2001) 11: 2627-2630 [PMID:11551765]
ChEMBL	Inhibition of rat cortex AChE using acetylthiocholine iodide as substrate after 20 mins by by Ellman's method	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem (2018) 26: 3117-3125 [PMID:29729987]
ChEMBL	Inhibition of rat AChE	B	6.94	pIC50	114	nM	IC50	J Med Chem (2008) 51: 3154-3170 [PMID:18479118]
ChEMBL	Inhibition of rat AChE	B	6.94	pIC50	114	nM	IC50	J Med Chem (2008) 51: 3154-3170 [PMID:18479118]
ChEMBL	Inhibitory potency against acetylcholinesterase (AChE) of rat cortex homogenate with ethopropazine as BChE inhibitor	B	6.94	pIC50	114	nM	IC50	Bioorg Med Chem Lett (1999) 9: 2335-2338 [PMID:10476864]
ChEMBL	Inhibition of AChE in rat cortex homogenate using acetylthiocholine iodide as substrate after 20 mins by Ellman's method	B	7.01	pIC50	98	nM	IC50	Eur J Med Chem (2018) 143: 33-47 [PMID:29172081]
ChEMBL	Inhibitory activity against acetylcholinesterase in rat brain	B	7.03	pIC50	94	nM	IC50	Bioorg Med Chem Lett (1996) 6: 737-742
ChEMBL	Inhibition of rat cortex homogenate acetylcholinesterase by spectrophotometric method	B	7.09	pIC50	82	nM	IC50	Bioorg Med Chem (2020) 28: 115820-115820 [PMID:33120080]
ChEMBL	Inhibition of rat cortex AChE using acetylthiocholine iodide as substrate measured after 20 mins by Ellman's method	B	7.09	pIC50	82	nM	IC50	J Med Chem (2016) 59: 8326-8344 [PMID:27552582]
ChEMBL	In vitro inhibitory activity against Acetylcholinesterase (AChE) using rat brain hippocampal crude homogenates.	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (1998) 8: 1661-1664 [PMID:9873409]
ChEMBL	Acetylcholinesterase inhibitory activity in rat striatal homogenates	B	7.1	pIC50	80	nM	IC50	J Med Chem (1995) 38: 3645-3651 [PMID:7658452]
ChEMBL	Inhibition of AChE in rat cortex at pH 5	B	7.14	pIC50	72.4	nM	IC50	Bioorg Med Chem (2007) 15: 1394-1408 [PMID:17126020]
ChEMBL	Inhibitory concentration against acetylcholinesterase of rat cortex homogenate	B	7.14	pIC50	72.4	nM	IC50	J Med Chem (2005) 48: 655-657 [PMID:15689148]
ChEMBL	Compound was tested for its ability to inhibit the action of acetylcholinesterase isolated from rat brain cortex	B	7.15	pIC50	71.5	nM	IC50	J Med Chem (1991) 34: 3399-3402 [PMID:1766006]
ChEMBL	Inhibition of AChE in Sprague-Dawley rat cortices using acetylthiocholine iodide as substrate by Ellman's method	B	7.19	pIC50	65	nM	IC50	Bioorg Med Chem (2013) 21: 676-683 [PMID:23273608]
ChEMBL	In vitro inhibitory concentration against rat cortex acetylcholinesterase	B	7.28	pIC50	53	nM	IC50	Bioorg Med Chem Lett (2005) 15: 3834-3837 [PMID:15993600]
ChEMBL	Compound was tested for its ability to inhibit the action of acetylcholinesterase isolated from rat brain cortex	B	7.35	pIC50	44.5	nM	IC50	J Med Chem (1991) 34: 3399-3402 [PMID:1766006]
Butyrylcholinesterase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3403] [UniProtKB: Q9JKC1]
ChEMBL	Inhibition of rat cortex homogenate BuChE by spectrophotometric method	B	4.13	pIC50	74430	nM	IC50	Bioorg Med Chem (2020) 28: 115820-115820 [PMID:33120080]
ChEMBL	Inhibition of BuChE in rat serum	B	4.15	pIC50	70200	nM	IC50	Bioorg Med Chem (2007) 15: 1394-1408 [PMID:17126020]
ChEMBL	In vitro inhibitory concentration against butyrylcholinesterase was determined using rat serum homogenate	B	4.25	pIC50	56200	nM	IC50	Bioorg Med Chem Lett (2005) 15: 3834-3837 [PMID:15993600]
ChEMBL	Inhibition of rat serum BChE using butyrylthiocholine iodide as substrate after 20 mins by by Ellman's method	B	4.44	pIC50	36400	nM	IC50	Bioorg Med Chem (2018) 26: 3117-3125 [PMID:29729987]
ChEMBL	Inhibition of BuChE in rat serum using butyrylthiocholine iodide as substrate after 20 mins by Ellman's method	B	4.44	pIC50	36400	nM	IC50	Eur J Med Chem (2018) 143: 33-47 [PMID:29172081]
butyrylcholinesterase/Cholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276]
ChEMBL	Inhibition of human BuChE	B	6	pKi	>1000	nM	Ki	J Med Chem (2006) 49: 3421-3425 [PMID:16722663]
ChEMBL	Inhibition of BuChE (unknown origin) using butyrylthiocholine iodide as substrate by DTNB reagent based Ellman's method	B	2	pIC50	>10000000	nM	IC50	Bioorg Med Chem Lett (2022) 72: 128873-128873 [PMID:35779827]
ChEMBL	Inhibition of butyrylcholinesterase (BChE) activity in human serum	B	4	pIC50	>100000	nM	IC50	J Med Chem (1999) 42: 3227-3242 [PMID:10464010]
ChEMBL	Inhibition of human BuChE using butyrylthiocholine iodide as substrate measured for 1 min by Ellman's method	B	4	pIC50	>100000	nM	IC50	J Nat Prod (2019) 82: 239-248 [PMID:30701972]
ChEMBL	Inhibitory concentration against human plasma Butyrylcholinesterase	B	5	pIC50	>10000	nM	IC50	J Med Chem (2005) 48: 986-994 [PMID:15715468]
ChEMBL	Ex vivo inhibition of human plasma Butyrylcholinesterase.	B	5	pIC50	>10000	nM	IC50	J Med Chem (2002) 45: 3684-3691 [PMID:12166941]
ChEMBL	Anticholinesterase activity against human plasma BChE	B	5	pIC50	>10000	nM	IC50	J Med Chem (2006) 49: 2174-2185 [PMID:16570913]
ChEMBL	Inhibition of human serum BChE	B	5	pIC50	>10000	nM	IC50	J Med Chem (2006) 49: 6833-6840 [PMID:17154513]
ChEMBL	Inhibitory concentration against Butyrylcholinesterase from human erythrocytes	B	5	pIC50	>10000	nM	IC50	J Med Chem (2005) 48: 1701-1704 [PMID:15771413]
ChEMBL	Inhibition of butyrylcholinesterase (BChE) in human serum	B	5	pIC50	>10000	nM	IC50	J Med Chem (2000) 43: 4657-4666 [PMID:11101357]
ChEMBL	In vitro inhibitory concentration against BChE of human serum	B	5	pIC50	>10000	nM	IC50	J Med Chem (2001) 44: 4733-4736 [PMID:11741490]
ChEMBL	Inhibition of BChE	B	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2687-2691 [PMID:21216144]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]