ChEMBL ligand: CHEMBL134713 (3-(2,4-Dimethoxybenzylidene)Anabaseine, Dimethoxybenzylidene anabaseine, Dmxb-a, Gts-21)
Acetylcholine-binding protein in Lymnaea stagnalis [ChEMBL: CHEMBL6084] [UniProtKB: P58154] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor α1 subunit/nicotinic acetylcholine receptor γ subunit/nicotinic acetylcholine receptor β1 subunit/nicotinic acetylcholine receptor δ subunit/Acetylcholine receptor; alpha1/beta1/delta/gamma in Human [ChEMBL: CHEMBL1907588] [GtoPdb: 462, 475, 471, 476] [UniProtKB: P02708, P07510, P11230, Q07001] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor β4 subunit/nicotinic acetylcholine receptor α3 subunit/Neuronal acetylcholine receptor; alpha3/beta4 in Human [ChEMBL: CHEMBL1907594] [GtoPdb: 474, 464] [UniProtKB: P30926, P32297] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor β2 subunit/nicotinic acetylcholine receptor α4 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Human [ChEMBL: CHEMBL1907589] [GtoPdb: 472, 465] [UniProtKB: P17787, P43681] nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Rat [ChEMBL: CHEMBL1907596] [GtoPdb: 465, 472] [UniProtKB: P09483, P12390] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor β4 subunit/nicotinic acetylcholine receptor α4 subunit/Neuronal acetylcholine receptor; alpha4/beta4 in Human [ChEMBL: CHEMBL1907591] [GtoPdb: 474, 465] [UniProtKB: P30926, P43681] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor α7 subunit/Neuronal acetylcholine receptor protein alpha-7 subunit in Human [ChEMBL: CHEMBL2492] [GtoPdb: 468] [UniProtKB: P36544] nicotinic acetylcholine receptor α7 subunit/Neuronal acetylcholine receptor protein alpha-7 subunit in Rat [ChEMBL: CHEMBL4980] [GtoPdb: 468] [UniProtKB: Q05941] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Acetylcholine-binding protein in Lymnaea stagnalis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6084] [UniProtKB: P58154]
ChEMBL	Displacement of [3H]-epibatidine from Lymnaea stagnalis N-terminal FLAG-tagged AChBP expressed in HEK293 cells after 2 hrs by liquid scintillation counting	B	7.72	pKd	19	nM	Kd	Eur J Med Chem (2011) 46: 5625-5635 [PMID:21986237]
nicotinic acetylcholine receptor α1 subunit/nicotinic acetylcholine receptor γ subunit/nicotinic acetylcholine receptor β1 subunit/nicotinic acetylcholine receptor δ subunit/Acetylcholine receptor; alpha1/beta1/delta/gamma in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907588] [GtoPdb: 462, 475, 471, 476] [UniProtKB: P02708, P07510, P11230, Q07001]
ChEMBL	Displacement of [3H]-epibatidine from alpha1betagammadelta nAChR in human TE671 cellular membranes after 2 hrs by liquid scintillation counting	B	5.41	pKi	3900	nM	Ki	Eur J Med Chem (2011) 46: 5625-5635 [PMID:21986237]
ChEMBL	Displacement of [3H]epibatidine from human alpha1/beta/gamma/delta nAchR expressed in TE-671 cell membrane	B	5.41	pKi	3900	nM	Ki	Bioorg Med Chem Lett (2012) 22: 1179-1186 [PMID:22177081]
nicotinic acetylcholine receptor β4 subunit/nicotinic acetylcholine receptor α3 subunit/Neuronal acetylcholine receptor; alpha3/beta4 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907594] [GtoPdb: 474, 464] [UniProtKB: P30926, P32297]
ChEMBL	Displacement of [3H]-epibatidine from alpha3beta4 nAChR in human SH-SY5Y cellular membranes after 2 hrs by liquid scintillation counting	B	5.6	pKi	2500	nM	Ki	Eur J Med Chem (2011) 46: 5625-5635 [PMID:21986237]
ChEMBL	Displacement of [3H]epibatidine from human alpha3beta4 nAchR expressed in SH-SY5Y cells	B	5.6	pKi	2500	nM	Ki	Bioorg Med Chem Lett (2012) 22: 1179-1186 [PMID:22177081]
ChEMBL	Effective concentration against Nicotinic acetylcholine receptor alpha3-beta4	B	5	pEC50	>10000	nM	EC50	J Med Chem (2005) 48: 4705-4745 [PMID:16033252]
nicotinic acetylcholine receptor β2 subunit/nicotinic acetylcholine receptor α4 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907589] [GtoPdb: 472, 465] [UniProtKB: P17787, P43681]
ChEMBL	Displacement of [3H]epibatidine from human alpha4beta2 nAchR expressed in SH-EP1 cells	B	7.29	pKi	51	nM	Ki	Bioorg Med Chem Lett (2012) 22: 1179-1186 [PMID:22177081]
ChEMBL	Effective concentration against Nicotinic acetylcholine receptor alpha4-beta2	B	5	pEC50	>10000	nM	EC50	J Med Chem (2005) 48: 4705-4745 [PMID:16033252]
nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907596] [GtoPdb: 465, 472] [UniProtKB: P09483, P12390]
ChEMBL	Displacement of [3H]cytisine from alpha4beta2 nAChR in rat brain membrane after 3 hrs	B	0.6	pKi	0.6	uM	pKi	Eur J Med Chem (2010) 45: 2433-2446 [PMID:20236734]
ChEMBL	Displacement of [3H]cytisine from alpha4beta2 nAChR in rat brain membrane after 3 hrs	B	6.6	pKi	250	nM	Ki	Eur J Med Chem (2010) 45: 2433-2446 [PMID:20236734]
ChEMBL	Inhibition of [3H]cytisine binding to nicotinic acetylcholine receptor alpha4-beta2 from rat cerebral cortex	B	7.08	pKi	84	nM	Ki	J Med Chem (2005) 48: 2678-2686 [PMID:15801858]
ChEMBL	Displacement of [3H]-epibatidine from alpha4beta2 nAChR in rat cortical membranes after 2 hrs by liquid scintillation counting	B	7.29	pKi	51	nM	Ki	Eur J Med Chem (2011) 46: 5625-5635 [PMID:21986237]
nicotinic acetylcholine receptor β4 subunit/nicotinic acetylcholine receptor α4 subunit/Neuronal acetylcholine receptor; alpha4/beta4 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907591] [GtoPdb: 474, 465] [UniProtKB: P30926, P43681]
ChEMBL	Effective concentration against Nicotinic acetylcholine receptor alpha4-beta4	B	5	pEC50	>10000	nM	EC50	J Med Chem (2005) 48: 4705-4745 [PMID:16033252]
nicotinic acetylcholine receptor α7 subunit/Neuronal acetylcholine receptor protein alpha-7 subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2492] [GtoPdb: 468] [UniProtKB: P36544]
ChEMBL	Displacement of [125I]alpha-bungarotoxin from alpha7 nAChR	B	6.8	pKd	158	nM	Kd	J Med Chem (2008) 51: 6293-6302 [PMID:18826295]
ChEMBL	Partial agonist activity at human alpha7 nAChR expressed in Xenopus laevis oocytes incubated for 90 secs followed by washout period for 5 mins by electrophysiological method	B	5.22	pEC50	6000	nM	EC50	Eur J Med Chem (2018) 160: 37-48 [PMID:30317024]
ChEMBL	Agonist activity at human recombinant alpha7 nAChR expressed in rat GH3 cells by calcium influx assay	F	5.7	pEC50	1995.26	nM	EC50	Bioorg Med Chem Lett (2009) 19: 1287-1291 [PMID:19208472]
nicotinic acetylcholine receptor α7 subunit/Neuronal acetylcholine receptor protein alpha-7 subunit in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4980] [GtoPdb: 468] [UniProtKB: Q05941]
ChEMBL	Displacement of [125I]-alpha-bungarotoxin from alpha7 nAChR in rat brain membrane after 3 hrs	B	0.89	pKi	0.89	uM	pKi	Eur J Med Chem (2010) 45: 2433-2446 [PMID:20236734]
ChEMBL	Displacement of [3H]-methyllycaconitine from alpha7 nAChR in rat hippocampal membranes after 2 hrs by liquid scintillation counting	B	5.89	pKi	1300	nM	Ki	Eur J Med Chem (2011) 46: 5625-5635 [PMID:21986237]
ChEMBL	Inhibition of [125I]alpha-bungarotoxin binding to rat nicotinic acetylcholine receptor alpha7	B	6.66	pKi	220	nM	Ki	J Med Chem (2005) 48: 2678-2686 [PMID:15801858]
GtoPdb	Inhibition of [125I]alpha-bungarotoxin binding to rat α nAChR	-	6.66	pKi	220	nM	Ki	J Med Chem (2005) 48: 2678-86 [PMID:15801858]
ChEMBL	Displacement of [125I]-alpha-bungarotoxin from alpha7 nAChR in rat brain membrane after 3 hrs	B	6.89	pKi	130	nM	Ki	Eur J Med Chem (2010) 45: 2433-2446 [PMID:20236734]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]