ChEMBL ligand: CHEMBL254129 ([3H]-Cyclopamine, Cyclopamine)
SMO/Smoothened homolog in Human [ChEMBL: CHEMBL5971] [GtoPdb: 239] [UniProtKB: Q99835] SMO/Smoothened homolog in Mouse [ChEMBL: CHEMBL6080] [GtoPdb: 239] [UniProtKB: P56726] There should be some charts here, you may need to enable JavaScript!
Sonic hedgehog protein in Human [ChEMBL: CHEMBL5602] [UniProtKB: Q15465] Sonic hedgehog protein in Mouse [ChEMBL: CHEMBL5387] [UniProtKB: Q62226] There should be some charts here, you may need to enable JavaScript!
Zinc finger protein GLI1 in Human [ChEMBL: CHEMBL5461] [UniProtKB: P08151] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
SMO/Smoothened homolog in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5971] [GtoPdb: 239] [UniProtKB: Q99835]
ChEMBL	Binding affinity to Smo D473H mutant expressed in U2OS cells by scintillation counting	B	6.94	pKd	116	nM	Kd	Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522]
ChEMBL	Binding affinity to wild type Smo expressed in U2OS cells by scintillation counting	B	7.91	pKd	12.4	nM	Kd	Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522]
ChEMBL	Displacement of [3H]cyclopamine from Smo D473H mutant expressed in U2OS cells after 2 hrs by scintillation counting	B	6.63	pKi	232	nM	Ki	Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522]
ChEMBL	Displacement of [3H]-cyclopamine from SMO V404M mutant in gefitinib resistant human HCC827 cells by scintillation counting	B	7.29	pKi	51	nM	Ki	J Med Chem (2017) 60: 7447-7458 [PMID:28787156]
ChEMBL	Displacement of [3H]cyclopamine from wild type Smo expressed in U2OS cells after 2 hrs by scintillation counting	B	7.9	pKi	12.7	nM	Ki	Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522]
ChEMBL	Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assay	F	6.52	pIC50	300	nM	IC50	J Med Chem (2012) 55: 1559-1571 [PMID:22268551]
ChEMBL	Antagonist activity at human Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	B	6.55	pIC50	280	nM	IC50	Bioorg Med Chem Lett (2009) 19: 328-331 [PMID:19091559]
ChEMBL	Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopy	B	7.19	pIC50	64	nM	IC50	J Med Chem (2012) 55: 1559-1571 [PMID:22268551]
SMO/Smoothened homolog in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6080] [GtoPdb: 239] [UniProtKB: P56726]
ChEMBL	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay	B	5.72	pIC50	1900	nM	IC50	ACS Med Chem Lett (2012) 3: 808-813 [PMID:23074541]
ChEMBL	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli1 transcriptional activity	B	5.82	pIC50	1500	nM	IC50	ACS Med Chem Lett (2012) 3: 808-813 [PMID:23074541]
ChEMBL	Antagonist activity at mouse Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	B	5.92	pIC50	1200	nM	IC50	Bioorg Med Chem Lett (2009) 19: 328-331 [PMID:19091559]
ChEMBL	Antagonist activity at Smo in mouse Shh-Light 2 cells assessed as inhibition of Shh-induced Gli1-reporter activity after 2 days by dual-luciferase reporter gene method	F	6.32	pIC50	484	nM	IC50	Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522]
GtoPdb	Measuring inhibition of Hh pathway signalling in Gli-dependent reporter assays in Shh-LIGHT2 cells.	-	7	pIC50	~100	nM	IC50	Bioorg Med Chem (2016) 24: 3752-7 [PMID:27338657]
Sonic hedgehog protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5602] [UniProtKB: Q15465]
ChEMBL	Inhibition of human Shh-induced mouse C3H10T1/2 cell differentiation after 48 hrs by alkaline phosphatase reporter assay	B	6.22	pIC50	600	nM	IC50	ACS Med Chem Lett (2012) 3: 808-813 [PMID:23074541]
Sonic hedgehog protein in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5387] [UniProtKB: Q62226]
ChEMBL	Inhibition of SHH in mouse Shh-Light2 cells after 48 hrs by Gli1 reporter gene assay in presence of SAG	F	5.88	pIC50	1312	nM	IC50	Bioorg Med Chem (2009) 17: 4943-4954 [PMID:19541490]
ChEMBL	Inhibition of N-terminal SHH activated pathway in mouse C3H10T1/2 cells assessed as SAG-induced cell differentiation by alkaline phosphatase assay	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2011) 21: 3608-3612 [PMID:21592788]
ChEMBL	Inhibition of SHH pathway in mouse Shh Light2 cells after 40 hrs by Gli-dependent luciferase reporter gene assay	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2011) 21: 3608-3612 [PMID:21592788]
ChEMBL	Inhibition of SHH in mouse Shh Light2 cells by GLI-responsive firefly luciferase reporter gene assay	F	6.3	pEC50	500	nM	EC50	J Med Chem (2009) 52: 3829-3845 [PMID:19309080]
Zinc finger protein GLI1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5461] [UniProtKB: P08151]
ChEMBL	Inhibition of 100 nM SAG-stimulated Gli1 (unknown origin)-mediated transcriptional activity expressed in mouse Shh Light2 cells after 48 hrs by luciferase reporter gene assay	B	5.3	pIC50	5000	nM	IC50	Medchemcomm (2016) 7: 2328-2331

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]