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DNA gyrase subunit B

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Target not currently curated in GtoImmuPdb

Target id: 3218

Nomenclature: DNA gyrase subunit B

Family: Bacterial protein targets

Contents:

Quaternary Structure: Complexes
DNA gyrase
Gene and Protein Information Click here for help
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Escherichia coli - 804 gyrB DNA gyrase subunit B
Previous and Unofficial Names Click here for help
Type IIA topoisomerase subunit GyrB
Database Links Click here for help
Alphafold P0AES6 (E.coli)
ChEMBL Target CHEMBL1826 (E.coli)
Entrez Gene 948211 (E.coli)
RefSeq Protein YP_026241 (E.coli)
UniProtKB P0AES6 (E.coli)

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Inhibitors
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Reference
cyclothialidine Small molecule or natural product E.coli Competitive 8.2 pKi 3
⤷ pKi 8.2 (Ki 6x10-9 M) Cyclothialidine competitively inhibits the ATPase activity of DNA gyrase [3]
novobiocin Small molecule or natural product Approved drug Ligand has a PDB structure E.coli Competitive 7.1 pIC50 1
⤷ pIC50 7.1 (IC50 8x10-8 M) [1]
Description: DNA gyrase supercoiling assay
Whole Organism Assay Data Linked to This Target
Key to terms and symbols Click column headers to sort
Ligand Sp. Assay description Type Action Value Parameter Reference
cyclothialidine Small molecule or natural product E.coli - Inhibition - - 2
⤷ MIC >128 μg/ml [2]
General Comments
DNA gyrase subunit B is comprised of an N-terminal domain (43 kDa) containing the ATPase activity, and a C-terminal domain (47 kDa) involved in interactions with subunit A and DNA.

References

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1. Alt S, Mitchenall LA, Maxwell A, Heide L. (2011) Inhibition of DNA gyrase and DNA topoisomerase IV of Staphylococcus aureus and Escherichia coli by aminocoumarin antibiotics. J Antimicrob Chemother, 66 (9): 2061-9. [PMID:21693461]

2. Bisacchi GS, Manchester JI. (2015) A New-Class Antibacterial-Almost. Lessons in Drug Discovery and Development: A Critical Analysis of More than 50 Years of Effort toward ATPase Inhibitors of DNA Gyrase and Topoisomerase IV. ACS Infect Dis, 1 (1): 4-41. [PMID:27620144]

3. Nakada N, Gmünder H, Hirata T, Arisawa M. (1994) Mechanism of inhibition of DNA gyrase by cyclothialidine, a novel DNA gyrase inhibitor. Antimicrob Agents Chemother, 38 (9): 1966-73. [PMID:7811004]

How to cite this page

Bacterial protein targets: DNA gyrase subunit B. Last modified on 25/04/2023. Accessed on 28/04/2024. IUPHAR/BPS Guide to PHARMACOLOGY, https://www.guidetoimmunopharmacology.org/GRAC/ObjectDisplayForward?objectId=3218.