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ADGRG5

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Target not currently curated in GtoImmuPdb

Target id: 194

Nomenclature: ADGRG5

Family: Adhesion Class GPCRs

Gene and Protein Information Click here for help
Adhesion G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 528 16q21 ADGRG5 adhesion G protein-coupled receptor G5
Mouse 7 524 8 C5 Adgrg5 adhesion G protein-coupled receptor G5
Rat 7 523 19p13 Adgrg5 adhesion G protein-coupled receptor G5
Previous and Unofficial Names Click here for help
Gm1109 | GPR114 (G protein-coupled receptor 114) | PGR27 | UNQ2524/PRO6017
Database Links Click here for help
Specialist databases
GPCRDB agrg5_human (Hs), agrg5_mouse (Mm)
Other databases
ChEMBL Target
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
Pharos
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Endogenous agonists
Peptides derived from the Stachel sequence: TYFAVLMQLSGDPVPAEL  [8-9]
Agonist Comments
Peptides derived from the Stachel sequence: TYFAVLMQLSGDPVPAEL [8-9].
Antagonist Comments
Dihydromunduletone, a rotenoid derivative, is an antagonist at ADGRG5 [8].
Cell Type Associations
Immuno Cell Type:  Natural killer cells
References:  7
Immuno Cell Type:  Granulocytes
Cell Ontology Term:   granulocyte (CL:0000094)
eosinophil (CL:0000771)
Comment:  Amongst immune cells types, ADGRG5 is expressed primarily by granulocytes such as eosinophils.
References:  7
Primary Transduction Mechanisms Click here for help
Transducer Effector/Response
Gs family
References:  5,8-9
Tissue Distribution Click here for help
Unigene expression profile highest in lymph node, bone marrow, tonsil, thymus, colon, pancreas.
Species:  Human
Technique: 
References: 
Colon, lung, spleen, thymus, pancreas islets of Langerhans, leukocytes, leukemia cells
Species:  Human
Technique:  EST
References:  1-3
Leukocytes
Species:  Human
Technique:  Microarray analysis
References:  7
Unigene expression proifile highest in blood, spleen, inner ear, thymus.
Species:  Mouse
Technique: 
References: 
Expression Datasets Click here for help

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays Click here for help
Elevated levels of cAMP
Species:  Human
Tissue:  293T cell line
Response measured:  Receptor causes constitutive activation of GαS G protein
References:  5
ADGRG5 couples to Gs protein.
Species:  Human
Tissue:  COS-7 cells.
Response measured:  Proximity luminescence (AlphaScreen assay), GTPγS binding
References:  8
General Comments
ADGRG5 (formerly GPR114) is an orphan receptor belonging to Family VIII Adhesion-GPCRs together with ADGRG1-4, 6 and 7 [3]. The genes of ADGRG1, ADGRG3 and ADGRG5 are syntenically clustered on human chromosome 16 and mouse chromosome 8 suggesting the evolution from an ancestral gene through gene duplication and exon shuffling [7]. Adgrg5 is mechano-sensitive and has two splice variants in mouse which significantly differ in their Gs-mediated basal activity [9].

Full coding sequence human cDNA is publicly available, IMAGE:5210813 [6], in mammalian expression vector pCMV-SPORT6.

Original human gene prediction, AY140956 [4] incorrectly predicts the 3' end of the gene resulting in a longer protein, (Swiss-Prot ID: Q8IZF4, 570 aa), than the observed cDNAs.

References

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1. Bjarnadóttir TK, Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB. (2004) The human and mouse repertoire of the adhesion family of G-protein-coupled receptors. Genomics, 84 (1): 23-33. [PMID:15203201]

2. Bjarnadóttir TK, Geirardsdóttir K, Ingemansson M, Mirza MA, Fredriksson R, Schiöth HB. (2007) Identification of novel splice variants of Adhesion G protein-coupled receptors. Gene, 387 (1-2): 38-48. [PMID:17056209]

3. Fredriksson R, Gloriam DE, Höglund PJ, Lagerström MC, Schiöth HB. (2003) There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini. Biochem Biophys Res Commun, 301 (3): 725-34. [PMID:12565841]

4. Fredriksson R, Lagerström MC, Höglund PJ, Schiöth HB. (2002) Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions. FEBS Lett, 531 (3): 407-14. [PMID:12435584]

5. Gupte J, Swaminath G, Danao J, Tian H, Li Y, Wu X. (2012) Signaling property study of adhesion G-protein-coupled receptors. FEBS Lett, 586 (8): 1214-9. [PMID:22575658]

6. Lennon G, Auffray C, Polymeropoulos M, Soares MB. (1996) The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics, 33 (1): 151-2. [PMID:8617505]

7. Peng YM, van de Garde MD, Cheng KF, Baars PA, Remmerswaal EB, van Lier RA, Mackay CR, Lin HH, Hamann J. (2011) Specific expression of GPR56 by human cytotoxic lymphocytes. J Leukoc Biol, 90 (4): 735-40. [PMID:21724806]

8. Stoveken HM, Bahr LL, Anders MW, Wojtovich AP, Smrcka AV, Tall GG. (2016) Dihydromunduletone Is a Small-Molecule Selective Adhesion G Protein-Coupled Receptor Antagonist. Mol Pharmacol, 90 (3): 214-24. [PMID:27338081]

9. Wilde C, Fischer L, Lede V, Kirchberger J, Rothemund S, Schöneberg T, Liebscher I. (2016) The constitutive activity of the adhesion GPCR GPR114/ADGRG5 is mediated by its tethered agonist. FASEB J, 30 (2): 666-73. [PMID:26499266]

Contributors

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