Z-FA-FMK   Click here for help

GtoPdb Ligand ID: 11257

Synonyms: MDL 201053 | MDL-201053 | MDL201053 | Z-Phe-DL-Ala-fluoromethylketone | ZFA-fmk
Immunopharmacology Ligand
Compound class: Peptide
Comment: Z-FA-FMK is a modified di-peptide. It has been reported as a cathepsin B inhibtor [1], and antiviral activity against a variety of viruses has been reported [2].

SARS-CoV-2: In an HTS screening programme Z-FA-FMK was found to inhibit the 3CL protease (Mpro) of SARS-CoV-2 [3]. This inhibition translated to an antiviral effect with an EC50 of 130 nM.
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Ligand Activity Visualisation Charts

These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. Click on a plot to see the median, interquartile range, low and high data points. A value of zero indicates that no data are available. A separate chart is created for each target, and where possible the algorithm tries to merge ChEMBL and GtoPdb targets by matching them on name and UniProt accession, for each available species. However, please note that inconsistency in naming of targets may lead to data for the same target being reported across multiple charts.

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View interactive charts of activity data across species

Z-FA-FMK is commercially available from our sponsors

2D Structure
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2D Structure

An image of the ligand's 2D structure. For small molecules with SMILES these are drawn using the NCI/CADD Chemical Identifier Resolver. Click on the image to access the chemical structure search tool with the ligand pre-loaded in the structure editor. For other types of ligands, e.g. longer nucleotides and peptides, a manually drawn representation of the molecule may be provided.

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SMILES / InChI / InChIKey
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SMILES / InChI / InChIKey

SMILES (Simplified Molecular Input Line Entry Specification) A specification for unambiguously describing the structure of chemical molecules using short ASCII strings. Canonical SMILES specify a unique representation of the 2D structure without chiral or isotopic specifications. Isomeric SMILES include chiral specification and isotopes.

Standard InChI (IUPAC International Chemical Identifier) and InChIKey InChI is a non-proprietary, standard, textual identifier for chemical substances designed to facilitate linking of information and database searching. An InChIKey is a simplified version of a full InChI, designed for easier web searching.

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)

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Canonical SMILES FCC(=O)C(NC(=O)[C@H](Cc1ccccc1)NC(=O)OCc1ccccc1)C
Isomeric SMILES FCC(=O)C(NC(=O)[C@H](Cc1ccccc1)NC(=O)OCc1ccccc1)C
InChI InChI=1S/C21H23FN2O4/c1-15(19(25)13-22)23-20(26)18(12-16-8-4-2-5-9-16)24-21(27)28-14-17-10-6-3-7-11-17/h2-11,15,18H,12-14H2,1H3,(H,23,26)(H,24,27)/t15?,18-/m0/s1
InChI Key ASXVEBPEZMSPHB-PKHIMPSTSA-N

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
References
1. Rajah T, Chow SC. (2015)
Suppression of Human T Cell Proliferation Mediated by the Cathepsin B Inhibitor, z-FA-FMK Is Due to Oxidative Stress.
PLoS ONE, 10 (4): e0123711. [PMID:25915766]
2. Roscow O, Ganassin R, Garver K, Polinski M. (2018)
Z-FA-FMK demonstrates differential inhibition of aquatic orthoreovirus (PRV), aquareovirus (CSRV), and rhabdovirus (IHNV) replication.
Virus Res, 244: 194-198. [PMID:29174718]
3. Zhu W, Xu M, Chen CZ, Guo H, Shen M, Hu X, Shinn P, Klumpp-Thomas C, Michael SG, Zheng W. (2020)
Identification of SARS-CoV-2 3CL Protease Inhibitors by a Quantitative High-throughput Screening.
ACS Pharmacol Transl Sci, [Epub ahead of print]. DOI: 10.1021/acsptsci.0c00108