ChEMBL ligand: CHEMBL210858
Macrophage migration inhibitory factor in Human [ChEMBL: CHEMBL2085] [UniProtKB: P14174] Macrophage migration inhibitory factor in Mouse [ChEMBL: CHEMBL1926491] [UniProtKB: P34884] There should be some charts here, you may need to enable JavaScript!
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Rat [ChEMBL: CHEMBL3602] [GtoPdb: 2552] [UniProtKB: Q9R0C9] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Macrophage migration inhibitory factor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2085] [UniProtKB: P14174]
ChEMBL	Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) preincubated for 10 mins followed by probe addition and measured after 10 mins by fluorescence assay	B	4.33	pKd	47000	nM	Kd	J Med Chem (2020) 63: 11920-11933 [PMID:32940040]
ChEMBL	Inhibition of C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) using 4-HPP as substrate preincubated for 10 mins followed by substrate addition by UV absorption spectrum analysis	B	4.36	pKi	44000	nM	Ki	J Med Chem (2020) 63: 11920-11933 [PMID:32940040]
ChEMBL	Inhibition of C-terminal His-tagged recombinant human MIF tautomerase activity expressed in Escherichia coli BL21 using 4-hydroxyphenyl pyruvic acid as substrate preincubated for 15 mins followed by substrate addition and measured for 10 mins in presence of 0.5 mM EDTA by UV absorbance based Cheng-Prusoff equation analysis	B	4.36	pKi	44000	nM	Ki	Eur J Med Chem (2020) 186: 111849-111849 [PMID:31767137]
ChEMBL	Inhibition of dopachrome tautomerase activity of human recombinant MIF	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4773-4776 [PMID:19592246]
ChEMBL	Inhibition of recombinant His-tagged MIF tautomerase activity (unknown origin) using 4-HPP as substrate	B	4.1	pIC50	79000	nM	IC50	Bioorg Med Chem (2018) 26: 999-1005 [PMID:29428527]
ChEMBL	Inhibition of dopachrome tautomerase activity of MIF in human THP1 cells	B	4.16	pIC50	69000	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4773-4776 [PMID:19592246]
ChEMBL	Inhibition of C-terminal His-tagged recombinant human MIF tautomerase activity expressed in Escherichia coli BL21 using 4-hydroxyphenyl pyruvic acid as substrate preincubated for 15 mins followed by substrate addition and measured for 10 mins in presence of 0.5 mM EDTA by UV absorbance analysis	B	4.19	pIC50	64000	nM	IC50	Eur J Med Chem (2020) 186: 111849-111849 [PMID:31767137]
ChEMBL	Inhibition of spontaneous secretion/release/recognition of MIF from freshly isolated human PBMC by ELISA	B	4.6	pIC50	25000	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4773-4776 [PMID:19592246]
ChEMBL	Inhibition of recombinant human MIF (Pro2 to Ala115 residues) expressed in Escherichia coli using dopachrome methyl ester as substrate preincubated with protein followed by substrate addition	B	4.74	pIC50	18200	nM	IC50	J Med Chem (2018) 61: 8104-8119 [PMID:29812929]
ChEMBL	Inhibition of MIF tautomerase activity	B	4.96	pIC50	11000	nM	IC50	Bioorg Med Chem Lett (2006) 16: 3376-3379 [PMID:16682188]
ChEMBL	Inhibition of recombinant MIF (unknown origin) tautomerase activity using dopachrome methyl ester as substrate measured for 2 to 20 secs by spectrophotometric analysis	B	5.15	pIC50	7000	nM	IC50	J Med Chem (2018) 61: 8104-8119 [PMID:29812929]
Macrophage migration inhibitory factor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1926491] [UniProtKB: P34884]
ChEMBL	Enzyme Assay: MIF tautomerase activity was measured by UV-Visible recording spectrophotometry (SHIMADZU, UV1600U). A fresh stock solution of L-dopachrome methyl ester was prepared at 2.4 mM through oxidation of L-3,4-dihydroxyphenylalanine methyl ester with sodium periodate. 1 μL of MIF solution (800-900 ng/mL) and 1 μL of a DMSO solution with various concentrations of the enzymatic inhibitor were added into a plastic cuvette (10 mm, 1.5 mL) containing 0.7 mL assay buffer (50 mM potassium phosphate, pH 7.2). Then L-dopachrome methyl ester solution (0.3 mL) was added to the assay buffer mixture. Activity was determined at room temperature and the spectrometric measurements were made at λ=475 nm for 20 seconds by monitoring the rate of decolorization of L-dopachrome methyl ester in comparison to a standard solution.	B	4.96	pIC50	11000	nM	IC50	US-8618147-B2. Modified macrophage migration inhibitory factor inhibitors (2013)
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3602] [GtoPdb: 2552] [UniProtKB: Q9R0C9]
ChEMBL	Displacement of [3H]-(+)-pentazocine from sigma 1 receptor in rat brain by competition binding assay	B	6.99	pKi	102.3	nM	Ki	Medchemcomm (2015) 6: 1093-1103

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]